House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance
Sponsor
China Medical University Hospital (Other)
Overall Status
Withdrawn
CT.gov ID
NCT03307278
Collaborator
(none)
0
2
9.2
Study Details
Study Description
Brief Summary
In this project, PBMCs will be isolated from HDM allergic and non allergic patient and then stimulated with HDM crude extract. The expression of inflammasome and the correlation of inflammasome activation and steroid resistance will be investigated.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Study Design
Study Type:
Interventional
Actual Enrollment
:
0 participants
Allocation:
Randomized
Intervention Model:
Parallel Assignment
Masking:
None (Open Label)
Primary Purpose:
Basic Science
Official Title:
Effects and Mechanism of House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance
Anticipated Study Start Date
:
Nov 30, 2017
Anticipated Primary Completion Date
:
Sep 6, 2018
Anticipated Study Completion Date
:
Sep 6, 2018
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: Steroid resistance in HDM allergic patients
|
Biological: House dust mite
As a stimulant to induce inflammasome activation
|
| Experimental: Steroid resistance in non HDM allergic subjects
|
Biological: House dust mite
As a stimulant to induce inflammasome activation
|
Outcome Measures
Primary Outcome Measures
- inflammasome activation by HDM [6 hours]
The expression of NLRP3 in PBMCs will be measured by western blot after HDM stimulation.
- Steroid receptor phosphorylation by HDM [6 hours]
The expression of steroid receptor will be detected by western blot and real-time PCR after HDM stimulation.
Eligibility Criteria
Criteria
Ages Eligible for Study:
20 Years
to 70 Years
Sexes Eligible for Study:
All
Accepts Healthy Volunteers:
Yes
Inclusion Criteria:
- HDM allergic and non allergic subjects
Exclusion Criteria:
- Pregnant subject, cancer
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- China Medical University Hospital
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.Responsible Party:
China Medical University Hospital
ClinicalTrials.gov Identifier:
NCT03307278
Other Study ID Numbers:
- CMUH106-REC3-115
First Posted:
Oct 11, 2017
Last Update Posted:
Aug 2, 2021
Last Verified:
Jul 1, 2021
Individual Participant Data (IPD) Sharing Statement:
Undecided
Plan to Share IPD:
Undecided
Studies a U.S. FDA-regulated Drug Product:
No
Studies a U.S. FDA-regulated Device Product:
No