Collecting and Storing Tissue From Young Patients With Cancer
Study Details
Study Description
Brief Summary
This laboratory study is collecting and storing tissue, blood, and bone marrow samples from young patients with cancer. Collecting and storing samples of tissue, blood, and bone marrow from patients with cancer to study in the laboratory may help doctors learn more about changes that may occur in DNA and identify biomarkers related to cancer.
Condition or Disease | Intervention/Treatment | Phase |
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Detailed Description
PRIMARY OBJECTIVES:
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Establish and bank cell lines and/or xenografts from pediatric patients with cancer.
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Establish continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer.
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Establish transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro.
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Create a bank of cell lines and generate sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols. V. Characterize cell lines from childhood cancers with respect to DNA short tandem repeat molecular profile as a "fingerprint" of original cell line identity.
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Characterize cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts.
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Characterize cell lines for mycoplasma contamination. VIII. Characterize cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance.
OUTLINE: This is a multicenter study.
Specimens are stratified according to disease (acute lymphoblastic leukemia vs acute myeloid leukemia vs lymphoma vs osteogenic sarcoma vs Ewing family of tumors vs rhabdomyosarcoma vs primitive neuroectodermal tumor vs glioma vs astrocytoma vs rhabdoid tumors vs hepatoblastoma vs retinoblastoma vs Wilms tumor vs germ cell tumors vs other diagnoses).
Leftover tissue from diagnostic procedures and/or surgery is cryopreserved and banked. Blood and/or bone marrow are also collected and banked. Cell lines are established and characterized via reverse-transcriptase polymerase chain reaction and/or flow cytometry for biomarkers and by DNA fingerprinting. Markers to be identified may include the following:
NEUROBLASTOMA: tyrosine hydroxylase, protein gene product (PGP) 9.5, GD2, HLA class I, and HSAN 1.2 antigens
EWING FAMILY OF TUMORS: EWS-FLI1, EWS-ERG, and PGP 9.5
RETINOBLASTOMA: interphotoreceptor retinoid-binding protein
ACUTE LYMPHOBLASTIC LEUKEMIA: immunophenotype
ALVEOLOR RHADOMYOSARCOMA: PAX3-FKHR, PAX7-FKHR, and MyoD1
ALL CELL TYPES: telomerase expression including hTR and hTERTMutations of TP53 gene are detected by flow cytometry and/or immunocytochemistry.
No results of these tests are provided to the patient, the patient's physician, or the patient's medical records.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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Ancillary-Correlative (tissue sample collection) Leftover tissue from diagnostic procedures and/or surgery is cryopreserved and banked. Blood and/or bone marrow are also collected and banked. Cell lines are established and characterized via reverse-transcriptase polymerase chain reaction and/or flow cytometry for biomarkers and by DNA fingerprinting. Markers to be identified may include the following: NEUROBLASTOMA: tyrosine hydroxylase, protein gene product (PGP) 9.5, GD2, HLA class I, and HSAN 1.2 antigens EWING FAMILY OF TUMORS: EWS-FLI1, EWS-ERG, and PGP 9.5 RETINOBLASTOMA: interphotoreceptor retinoid-binding protein ACUTE LYMPHOBLASTIC LEUKEMIA: immunophenotype ALVEOLOR RHADOMYOSARCOMA: PAX3-FKHR, PAX7-FKHR, and MyoD1 ALL CELL TYPES: telomerase expression including hTR and hTERTMutations of TP53 gene are detected by flow cytometry and/or immunocytochemistry |
Other: Cytology Specimen Collection Procedure
Correlative studies
Other Names:
Other: Laboratory Biomarker Analysis
Correlative studies
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Outcome Measures
Primary Outcome Measures
- Establishment and banking of cell lines and/or xenografts from pediatric patients with cancer [Up to 14 years]
- Establishment of continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer [Up to 14 years]
- Establishment of transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro [Up to 14 years]
- Creation of a bank of cell lines and generation of sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols [Up to 14 years]
- Characterization of cell lines from childhood cancers with respect to DNA PCR molecular HLA profile as a "fingerprint" of original cell line identity [Up to 14 years]
- Characterization of cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts [Up to 14 years]
- Characterization of cell lines for mycoplasma contamination [Up to 14 years]
- Characterization of cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance [Up to 14 years]
Eligibility Criteria
Criteria
Inclusion Criteria:
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All malignant tissues from childhood cancers allowed including the following:
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Brain tumors (all types)
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Tissue should be submitted to CNS Committee Resource labs to be forwarded for this study, unless instructed otherwise on the COG web site
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Ewing family of tumors
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Rhabdomyosarcomas
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Other soft tissue sarcomas
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Osteogenic sarcomas
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Rhabdoid tumors
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Neuroblastomas
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Viable material for cell culture for neuroblastoma is collected via COG-ANBL00B1 and should not be submitted via this study unless the patient cannot be enrolled on COG-ANBL00B1*
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Retinoblastomas
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Anaplastic Wilms tumor
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Germ cell tumors
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Leukemias/lymphomas
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Acute myeloid leukemia (AML)
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Blood samples and bone marrow samples from patients at second relapse and beyond may be submitted for this study
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Bone marrow samples at diagnosis or first relapse must be submitted to an AML resource lab and will be forwarded for this study at the discretion of the AML Committee
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Acute lymphoblastic leukemia (ALL)
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Blood samples may be submitted directly to this study
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Bone marrow samples must be submitted to an ALL resource lab and will be forwarded for this study at the discretion of the ALL Committee
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Enrolled on a COG therapeutic, biology, or tissue banking protocol that allows collection of tissue for research and submission to a COG-designated resource laboratory
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Participation in this protocol is not permitted until after tissue requirements for any active COG disease-specific therapeutic, biology, or banking protocols have been satisfied
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Material may only be submitted for this protocol if tissue is available in excess of that required for satisfying active disease-specific therapeutic and biological protocols
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Patients with diagnosis pending are eligible
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | Children's Hospital of Alabama | Birmingham | Alabama | United States | 35233 |
2 | University of Alabama at Birmingham Cancer Center | Birmingham | Alabama | United States | 35233 |
3 | Arkansas Children's Hospital | Little Rock | Arkansas | United States | 72202-3591 |
4 | University of Arkansas for Medical Sciences | Little Rock | Arkansas | United States | 72205 |
5 | Kaiser Permanente Downey Medical Center | Downey | California | United States | 90242 |
6 | City of Hope Comprehensive Cancer Center | Duarte | California | United States | 91010 |
7 | Miller Children's and Women's Hospital Long Beach | Long Beach | California | United States | 90806 |
8 | Children's Hospital Los Angeles | Los Angeles | California | United States | 90027 |
9 | Children's Hospital and Research Center at Oakland | Oakland | California | United States | 94609-1809 |
10 | Rocky Mountain Hospital for Children-Presbyterian Saint Luke's Medical Center | Denver | Colorado | United States | 80218 |
11 | Connecticut Children's Medical Center | Hartford | Connecticut | United States | 06106 |
12 | MedStar Georgetown University Hospital | Washington | District of Columbia | United States | 20007 |
13 | Lee Memorial Health System | Fort Myers | Florida | United States | 33901 |
14 | Golisano Children's Hospital of Southwest Florida | Fort Myers | Florida | United States | 33908 |
15 | Johns Hopkins All Children's Hospital | Saint Petersburg | Florida | United States | 33701 |
16 | Tampa General Hospital | Tampa | Florida | United States | 33606 |
17 | Saint Joseph's Hospital/Children's Hospital-Tampa | Tampa | Florida | United States | 33607 |
18 | Saint Mary's Hospital | West Palm Beach | Florida | United States | 33407 |
19 | Saint Luke's Mountain States Tumor Institute | Boise | Idaho | United States | 83712 |
20 | University of Illinois | Chicago | Illinois | United States | 60612 |
21 | Saint Jude Midwest Affiliate | Peoria | Illinois | United States | 61637 |
22 | Southern Illinois University School of Medicine | Springfield | Illinois | United States | 62702 |
23 | University of Kentucky/Markey Cancer Center | Lexington | Kentucky | United States | 40536 |
24 | Norton Children's Hospital | Louisville | Kentucky | United States | 40202 |
25 | Tulane University Health Sciences Center | New Orleans | Louisiana | United States | 70112 |
26 | Ochsner Medical Center Jefferson | New Orleans | Louisiana | United States | 70121 |
27 | Sinai Hospital of Baltimore | Baltimore | Maryland | United States | 21215 |
28 | Massachusetts General Hospital Cancer Center | Boston | Massachusetts | United States | 02114 |
29 | Dana-Farber Cancer Institute | Boston | Massachusetts | United States | 02215 |
30 | Wayne State University/Karmanos Cancer Institute | Detroit | Michigan | United States | 48201 |
31 | Bronson Methodist Hospital | Kalamazoo | Michigan | United States | 49007 |
32 | Kalamazoo Center for Medical Studies | Kalamazoo | Michigan | United States | 49008 |
33 | Columbia Regional | Columbia | Missouri | United States | 65201 |
34 | University of Missouri - Ellis Fischel | Columbia | Missouri | United States | 65212 |
35 | Children's Mercy Hospitals and Clinics | Kansas City | Missouri | United States | 64108 |
36 | Children's Hospital and Medical Center of Omaha | Omaha | Nebraska | United States | 68114 |
37 | University of Nebraska Medical Center | Omaha | Nebraska | United States | 68198 |
38 | University Medical Center of Southern Nevada | Las Vegas | Nevada | United States | 89102 |
39 | Nevada Cancer Research Foundation CCOP | Las Vegas | Nevada | United States | 89106 |
40 | Sunrise Hospital and Medical Center | Las Vegas | Nevada | United States | 89109 |
41 | Alliance for Childhood Diseases/Cure 4 the Kids Foundation | Las Vegas | Nevada | United States | 89135 |
42 | Summerlin Hospital Medical Center | Las Vegas | Nevada | United States | 89144 |
43 | Hackensack University Medical Center | Hackensack | New Jersey | United States | 07601 |
44 | Morristown Medical Center | Morristown | New Jersey | United States | 07960 |
45 | Overlook Hospital | Summit | New Jersey | United States | 07902 |
46 | Albany Medical Center | Albany | New York | United States | 12208 |
47 | Montefiore Medical Center - Moses Campus | Bronx | New York | United States | 10467 |
48 | NYP/Columbia University Medical Center/Herbert Irving Comprehensive Cancer Center | New York | New York | United States | 10032 |
49 | Carolinas Medical Center/Levine Cancer Institute | Charlotte | North Carolina | United States | 28203 |
50 | Wake Forest University Health Sciences | Winston-Salem | North Carolina | United States | 27157 |
51 | Children's Hospital Medical Center of Akron | Akron | Ohio | United States | 44308 |
52 | Rainbow Babies and Childrens Hospital | Cleveland | Ohio | United States | 44106 |
53 | Cleveland Clinic Foundation | Cleveland | Ohio | United States | 44195 |
54 | Dayton Children's Hospital | Dayton | Ohio | United States | 45404 |
55 | University of Oklahoma Health Sciences Center | Oklahoma City | Oklahoma | United States | 73104 |
56 | Children's Hospital of Pittsburgh of UPMC | Pittsburgh | Pennsylvania | United States | 15224 |
57 | BI-LO Charities Children's Cancer Center | Greenville | South Carolina | United States | 29605 |
58 | Greenville Cancer Treatment Center | Greenville | South Carolina | United States | 29605 |
59 | East Tennessee Childrens Hospital | Knoxville | Tennessee | United States | 37916 |
60 | Texas Tech University Health Sciences Center-Amarillo | Amarillo | Texas | United States | 79106 |
61 | Driscoll Children's Hospital | Corpus Christi | Texas | United States | 78411 |
62 | Covenant Children's Hospital | Lubbock | Texas | United States | 79410 |
63 | UMC Cancer Center / UMC Health System | Lubbock | Texas | United States | 79415 |
64 | Methodist Children's Hospital of South Texas | San Antonio | Texas | United States | 78229 |
65 | University of Texas Health Science Center at San Antonio | San Antonio | Texas | United States | 78229 |
66 | Madigan Army Medical Center | Tacoma | Washington | United States | 98431 |
67 | British Columbia Children's Hospital | Vancouver | British Columbia | Canada | V6H 3V4 |
68 | Centre Hospitalier Universitaire Sainte-Justine | Montreal | Quebec | Canada | H3T 1C5 |
69 | Saskatoon Cancer Centre | Saskatoon | Saskatchewan | Canada | S7N 4H4 |
Sponsors and Collaborators
- Children's Oncology Group
- National Cancer Institute (NCI)
Investigators
- Principal Investigator: Charles P Reynolds, Children's Oncology Group
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- ABTR04B1
- NCI-2009-00326
- CDR0000478867
- COG-ABTR04B1
- ABTR04B1
- ABTR04B1
- ABTR04B1
- U10CA098543