Effect of aPDT on Deep Caries in Permanent Tooth
Study Details
Study Description
Brief Summary
The aim of this study is to assess the clinical effect of aPDT on deep caries in permanent teeth. Half of the deep caries will receive aPDT disinfect in restorative treatment procedure, while the other half will receive sodium 0.9% saline in restorative treatment procedure.
Condition or Disease | Intervention/Treatment | Phase |
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N/A |
Detailed Description
The aim of the proposed study is to assess the clinical effect of antimicrobial photodynamic therapy (aPDT) using LED on dentin with deep caries in permanent teeth. Permanent teeth with deep carious lesions on the occlusal surface indicated for restorative treatment will be randomly allocated to two groups: Group 1 -Control after incomplete caries removal, washed with sodium 0.9% saline; Group 2 - PDT + CH after incomplete caries removal, disinfect the residual dentin with PDT(LED + O-Toluidine Blue). Dentin samples will be collected before and after disinfect for microbiological analysis. Then the teeth will be capped with CH and restored using resin with clinical and radiographic follow up at 6, 12 and 24 months.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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Experimental: Control Local anesthesia, rubber dam isolation, remove caries incompletely, microbiological sample with otoscope curette, dentin washed with sodium 0.9% saline, sampling again. Indirect pulp treatment with calcium hydroxide(CH). Restoration with resin(Z350,3M). Follow up at 6, 12 and 24 months. |
Procedure: Local anesthesia and rubber dam isolation
Local anesthesia was performed. Tooth was isolated using a rubber dam isolation in order to collect the dentin samples.
Procedure: Incomplete caries removal and microbiological samples collection
Partial carious tissue was removed with conventional dentinal curette and collection of dentin with a spoon (1#) was performed.
Procedure: Dentin washed with sodium 0.9% saline
The remaining dentin was dipped in sodium 0.9% saline for 2min.
Procedure: Microbiological samples collection and Indirect pulp treatment
A new dentin collection was performed after this treatment at another site of the cavity. Then teeth were conventionally restored using the following materials: calcium hydroxide lining material and resin (Z350,3M).
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Experimental: PDT+CH Local anesthesia, rubber dam isolation, remove caries incompletely, microbiological sample with otoscope curette, disinfect the remaining dentin with antimicrobial photodynamic therapy (DENFOTEX PADplus), sampling again. Indirect pulp treatment with calcium hydroxide(CH). Restoration with resin(Z350,3M). Follow up at 6, 12 and 24 months. |
Procedure: Local anesthesia and rubber dam isolation
Local anesthesia was performed. Tooth was isolated using a rubber dam isolation in order to collect the dentin samples.
Procedure: Incomplete caries removal and microbiological samples collection
Partial carious tissue was removed with conventional dentinal curette and collection of dentin with a spoon (1#) was performed.
Procedure: Antimicrobial Photodynamic Therapy
The remaining dentin was disinfected with aPDT for 2 min.
Procedure: Microbiological samples collection and Indirect pulp treatment
A new dentin collection was performed after this treatment at another site of the cavity. Then teeth were conventionally restored using the following materials: calcium hydroxide lining material and resin (Z350,3M).
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Outcome Measures
Primary Outcome Measures
- Quantification of bacteria by colony counting [2 days after dentin collection]
Quantification of total bacteria
Secondary Outcome Measures
- Tooth Pain [1 day and 6, 12, 24 months]
Patients reported the presence or absence of tooth pain
- Restoration Quality [1 day and 6, 12, 24 months]
The quality of restoration was evaluated through the absence of radiographic radiolucent area
Eligibility Criteria
Criteria
Inclusion Criteria:
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Good general health, without syndromes or chronic systemic diseases
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Permanent tooth with deep carious lesion (2/3 of dentin) without pain symptomatology and compatible with reversible pulpits
Exclusion Criteria:
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Refused to sign the informed consent document
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Allergic to photosensitizer
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Unlikely to be able to comply with the study procedures
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | School and Hospital of Stomatology, Fujian Medical University | Fuzhou | Fujian | China |
Sponsors and Collaborators
- Fujian Medical University
Investigators
- Study Chair: Xiaojing Huang, PhD, School and Hospital of stomatology, Fujian Medical University
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- PDT20160602