Studies of Disorders With Increased Susceptibility to Fungal Infections

Sponsor
National Institute of Allergy and Infectious Diseases (NIAID) (NIH)
Overall Status
Recruiting
CT.gov ID
NCT01222741
Collaborator
(none)
850
1

Study Details

Study Description

Brief Summary

Background:
  • Researchers are interested in studying disorders that make individuals more susceptible to fungal infections, specifically infections with the Candida yeast. These disorders are often related to problems with the immune system and may have genetic factors, which suggests that researchers should study not only the individual with the disorder, but also his or her first- and second-degree relatives (such as parents, siblings, children, and first cousins). To provide material for future research, individuals with immune disorders and their first- and second-degree relatives will be asked to provide blood and other samples for testing and comparison with samples taken from healthy volunteers with no history of immune disorders.
Objectives:
  • To collect blood and other biological samples to study immune disorders that make individuals more susceptible to fungal infections.
Eligibility:
  • Individuals of any age who have abnormal immune function characterized by recurrent or unusual fungal infections, recurrent or chronic inflammation, or other types of immune dysfunction.

  • First- or second-degree genetically related family members (limited to mother, father, siblings, grandparents, children, aunts, uncles, and first cousins).

  • Healthy volunteers at least 18 years of age (for comparison purposes).

Design:
  • Participants will provide blood samples and buccal (cells from the inside of the mouth near the cheek) samples.

  • Participants with immune disorders will also be asked to provide urine samples, saliva or mucosal samples, or skin tissue biopsies, and may also have imaging studies (such as x-rays) to collect information for research.

  • Samples may be collected at the National Institutes of Health or at other clinical locations for the samples to the sent to the National Institutes of Health.

  • No treatment will be provided as part of this protocol....

Condition or Disease Intervention/Treatment Phase

    Detailed Description

    This study is designed for the evaluation, diagnosis, and long-term follow up of selected patients with primary immune deficiencies and other conditions associated with fungal, and more specifically with Candida spp. infections. The primary immune deficiencies to be studied include, but are not limited to, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy (APECED), chronic mucocutaneous candidiasis (CMC), myeloperoxidase deficiency (MPO), immune dysregulation polyendocrinopathy enteropathy X-linked (IPEX), Job s syndrome, chronic granulomatous disease (CGD), and biotinidase deficiency. Diabetic patients and infants also show increased susceptibility to such infections and might be studied. Patient participants (who we will refer to as patients in this study) will undergo evaluations that include history/physical, blood sampling, genetic testing, and possible tissue sampling. We may use some of the blood cells to investigate the utility of induced pluripotent stem cells (iPS) for immune cell derivation and targeted gene correction. First or second degree genetically related family members (limited to mother, father, siblings, grandparents, children, aunts, uncles, and first cousins of an affected patient and who we will refer to as relatives in this study) might also be screened for clinical, in vitro, and genetic correlates of immune abnormalities. Healthy volunteers will be enrolled as a source of control samples for research testing. Among the aims of this protocol are to better understand the genetic and pathophysiologic factors that lead to defects in host defense, and to use modern and evolving methods in molecular and cellular biology to elucidate the pathogenesis of this particular susceptibility. A better understanding of primary immunodeficiency could allow for the rational development of novel therapies for such diseases and to benefit future patients, but it might not benefit current patient participants directly. Routine follow-up may occur every 6 months -with evaluation and blood sampling. Under some circumstances, we may provide treatment that relates to the immune deficiency. These treatments will follow standard medical practice.

    Study Design

    Study Type:
    Observational
    Anticipated Enrollment :
    850 participants
    Observational Model:
    Family-Based
    Time Perspective:
    Prospective
    Official Title:
    Studies of Disorders With Increased Susceptibility to Fungal Infections
    Actual Study Start Date :
    Jan 7, 2011

    Arms and Interventions

    Arm Intervention/Treatment
    Healthy Voluntary

    Healthy voluntary

    Patients

    affected patient

    relatives

    family member to patient

    Outcome Measures

    Primary Outcome Measures

    1. characterization [10 years]

      Characterize and compare the clinical and laboratory features of APECED, CMC, and other primary immunodeficiencies or particular conditions (such as infancy or diabetic subjects) with increased susceptibility to Candida or other fungal infections.

    2. Determine the prevalence of mutation [10 years]

      Determine the prevalence of AIRE mutations in patients with increased susceptibility to Candida or other fungal infections.

    3. genotype-phenotype correlation [10 years]

      Establish a genotype-phenotype correlation in patients with different AIRE mutations.

    4. Determine and compare the functionality [10 years]

      Determine and compare the functional integrity of Th17, Dectin1, and AIRE pathways in patients with increased susceptibility to Candida or other fungal infections with and without AIRE mutations

    Eligibility Criteria

    Criteria

    Ages Eligible for Study:
    N/A and Older
    Sexes Eligible for Study:
    All
    Accepts Healthy Volunteers:
    Yes
    • INCLUSION CRITERIA:
    Patient Participants (or simply Patient):

    Patients with abnormalities of immune function as manifested by recurrent or unusual fungal infections, recurrent or chronic inflammation, or previous laboratory evidence of immune dysfunction are eligible for screening and assessment under this protocol. Of particular focus of this study are patients with:

    • APECED (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)

    • CMC (chronic mucocutaneous candidiasis)

    • MPO (myeloperoxidase deficiency)

    • IPEX (immune dysregulation polyendocrinopathy enteropathy X-linked)

    • Hyper-immunoglobulin E syndrome (Job s syndrome)

    • CGD (chronic granulomatous disease)

    • Biotinidase deficiency

    • Other conditions showing increased susceptibility to such infections as described in infants and type 1 diabetic patientts

    There will be no limit due to age, sex, race, or disability.

    All patients must have a primary physician outside of the NIH.

    Women of child-bearing potential, or who are pregnant or lactating, may be eligible.

    All patients will be required to have blood stored for future studies and/or other medical conditions.

    Relatives:

    Relatives may be mother, father, siblings, children, grandparents, aunts, uncles, and first cousins to a patient participant.

    There is no limit due to age, sex, race, or disability.

    Relatives will be required to have blood stored for future studies and/or other medical conditions.

    Healthy volunteers must:

    Be an adult of either sex and between age of 18 and 85 years old

    Have a hemoglobin concentration of greater than or equal to 11 g/dL

    Weigh greater than or equal to 110 pounds

    EXCLUSION CRITERIA:
    Patient:

    The presence of certain types of acquired abnormalities of immunity solely due to human immunodeficiency virus (HIV), chemotherapeutic agent(s), or an underlying malignancy could be grounds for possible exclusion for a subject.

    Relatives:

    The presence of certain types of acquired abnormalities of immunity solely due to HIV, chemotherapeutic agent(s), or an underlying malignancy could be grounds for possible exclusion for a relative.

    Healthy volunteers are not eligible if:

    Receiving chemotherapeutic agent(s), or have underlying malignancy

    Pregnant or lactating

    Have history of heart, lung, kidney disease, bleeding or immunologic disorders leading to significant incapacity

    Have a history of recurrent or severe infections

    Have a history of HIV seropositivity

    Have a history of viral hepatitis (B or C)

    Contacts and Locations

    Locations

    Site City State Country Postal Code
    1 National Institutes of Health Clinical Center, 9000 Rockville Pike Bethesda Maryland United States 20892

    Sponsors and Collaborators

    • National Institute of Allergy and Infectious Diseases (NIAID)

    Investigators

    • Principal Investigator: Sergio D Rosenzweig, M.D., National Institute of Allergy and Infectious Diseases (NIAID)

    Study Documents (Full-Text)

    None provided.

    More Information

    Additional Information:

    Publications

    Responsible Party:
    National Institute of Allergy and Infectious Diseases (NIAID)
    ClinicalTrials.gov Identifier:
    NCT01222741
    Other Study ID Numbers:
    • 100216
    • 10-I-0216
    First Posted:
    Oct 18, 2010
    Last Update Posted:
    Aug 25, 2022
    Last Verified:
    Mar 23, 2022
    Individual Participant Data (IPD) Sharing Statement:
    Undecided
    Plan to Share IPD:
    Undecided
    Studies a U.S. FDA-regulated Drug Product:
    No
    Studies a U.S. FDA-regulated Device Product:
    No
    Keywords provided by National Institute of Allergy and Infectious Diseases (NIAID)
    Additional relevant MeSH terms:

    Study Results

    No Results Posted as of Aug 25, 2022