Comparison of the Genotoxicity of Desflurane and Propofol
Study Details
Study Description
Brief Summary
The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.
Condition or Disease | Intervention/Treatment | Phase |
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|
N/A |
Detailed Description
It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
---|---|
Experimental: Desflurane Group Desflurane 1,0 minimum alveolar concentration will be administered during anesthesia maintenance |
Drug: Anesthetics, General Desflurane Maintenance
Comparison of dna damage of desflurane maintenance with propofol maintenance according to comet analysis
|
Experimental: Propofol Group Propofol 6 mg//kg/hour will be administered during anesthesia maintenance |
Drug: Anesthetics, General Propofol Maintenance
Comparison of dna damage of propofol maintenance with desflurane maintenance according to comet analysis
|
Outcome Measures
Primary Outcome Measures
- Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [Before anesthesia induction]
The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.
- Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [2 hours after anesthesia induction]
The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.
- Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [postoperative 1st day]
The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.
- Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [postoperative 5th day]
The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.
- Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [Before anesthesia induction]
The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.
- Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [2 hours after anesthesia induction]
The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.
- Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [postoperative 1 st day]
The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.
- Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [postoperative 5th day]
The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.
- Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [before anesthesia induction]
The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.
- Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [2 hours after anesthesia induction]
The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.
- Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [postoperative 1 st day]
The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.
- Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire [postoperative 5th day]
The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Ability to understand and willingness to sign an informed consent form
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Elective lumbar spinal surgery that will take at least 120 minutes,
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American Society of Anesthesiologists (ASA) physical status I and II
Exclusion Criteria:
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Patients with ASA physical status III or IV disease
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Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders)
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Smoking or alcohol consumption
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Recently received radiation, chemotherapy
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Malignancy
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Patients who will receive blood transfusion during the operation
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Users of antioxidant supplements
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Patients who have received general anesthesia in the last three months
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Patients with known occupational exposures (operating room personnel, chemical plant worker
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
---|---|---|---|---|---|
1 | Mugla Sitki Kocman University | Mugla | Turkey | 48000 |
Sponsors and Collaborators
- Muğla Sıtkı Koçman University
Investigators
- Principal Investigator: Melike Korkmaz Toker, Assoc. Prof., Mugla Sıtkı Kocman University Department of Anesthesiology and reanimation
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- MSKU 05/III