Exercise-induced Changes in Exosomes
Study Details
Study Description
Brief Summary
The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.
Condition or Disease | Intervention/Treatment | Phase |
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N/A |
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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Experimental: Exercise trial Participants will perform a bout of exercise. In addition, a baseline and post-exercise blood sample will be drawn. |
Procedure: Resistance Exercise
Participants will perform a bout of lower body resistance exercise consisting of leg press, leg extension and leg curls.
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Outcome Measures
Primary Outcome Measures
- Collagen content of ligament constructs [15 min post-exercise]
Ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of collagen content using a hydroxyproline assay.
Secondary Outcome Measures
- Ultimate tensile stress and modulus of ligament constructs [15 min post-exercise]
ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of ultimate tensile stress and modulus.
- Exosomal RNA [15 min post-exercise]
Isolation and sequencing of exosomal RNA from serum obtained at baseline or post-exercise.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Young healthy adults (18-35 y)
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Experience with exercise (>1y)
Exclusion Criteria:
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Pregnancy
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Smoking
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Receiving any medication that may interfere with the study outcomes
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | Hickey Laboratory | Davis | California | United States | 95616 |
Sponsors and Collaborators
- University of California, Davis
Investigators
- Principal Investigator: Keith Baar, PhD, University of California, Davis
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 1956959