Exercise-induced Changes in Exosomes

Sponsor
University of California, Davis (Other)
Overall Status
Recruiting
CT.gov ID
NCT05616234
Collaborator
(none)
12
1
1
1.8
6.8

Study Details

Study Description

Brief Summary

The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.

Condition or Disease Intervention/Treatment Phase
  • Procedure: Resistance Exercise
N/A

Study Design

Study Type:
Interventional
Anticipated Enrollment :
12 participants
Allocation:
N/A
Intervention Model:
Single Group Assignment
Masking:
None (Open Label)
Primary Purpose:
Treatment
Official Title:
Exercise-induced Changes in Exosomes
Anticipated Study Start Date :
Nov 7, 2022
Anticipated Primary Completion Date :
Dec 31, 2022
Anticipated Study Completion Date :
Dec 31, 2022

Arms and Interventions

Arm Intervention/Treatment
Experimental: Exercise trial

Participants will perform a bout of exercise. In addition, a baseline and post-exercise blood sample will be drawn.

Procedure: Resistance Exercise
Participants will perform a bout of lower body resistance exercise consisting of leg press, leg extension and leg curls.

Outcome Measures

Primary Outcome Measures

  1. Collagen content of ligament constructs [15 min post-exercise]

    Ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of collagen content using a hydroxyproline assay.

Secondary Outcome Measures

  1. Ultimate tensile stress and modulus of ligament constructs [15 min post-exercise]

    ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of ultimate tensile stress and modulus.

  2. Exosomal RNA [15 min post-exercise]

    Isolation and sequencing of exosomal RNA from serum obtained at baseline or post-exercise.

Eligibility Criteria

Criteria

Ages Eligible for Study:
18 Years to 35 Years
Sexes Eligible for Study:
All
Accepts Healthy Volunteers:
Yes
Inclusion Criteria:
  • Young healthy adults (18-35 y)

  • Experience with exercise (>1y)

Exclusion Criteria:
  • Pregnancy

  • Smoking

  • Receiving any medication that may interfere with the study outcomes

Contacts and Locations

Locations

Site City State Country Postal Code
1 Hickey Laboratory Davis California United States 95616

Sponsors and Collaborators

  • University of California, Davis

Investigators

  • Principal Investigator: Keith Baar, PhD, University of California, Davis

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.
Responsible Party:
University of California, Davis
ClinicalTrials.gov Identifier:
NCT05616234
Other Study ID Numbers:
  • 1956959
First Posted:
Nov 15, 2022
Last Update Posted:
Nov 15, 2022
Last Verified:
Nov 1, 2022
Studies a U.S. FDA-regulated Drug Product:
No
Studies a U.S. FDA-regulated Device Product:
No

Study Results

No Results Posted as of Nov 15, 2022