Gene Expression Changes of Brain Tissue in Human Temporal Lobe Epilepsy With Hippocampal Sclerosis

Sponsor
Second Affiliated Hospital, School of Medicine, Zhejiang University (Other)
Overall Status
Active, not recruiting
CT.gov ID
NCT04917939
Collaborator
(none)
10
1
19.4
0.5

Study Details

Study Description

Brief Summary

Epilepsy is a common neurological disease, manifested in the sudden abnormal discharge of neurons leading to short-term brain dysfunction, has become the neurology after headache the second most common disease. In China, the prevalence of epilepsy is about 4.7-8.5 per 1,000, and more than 400,000 new cases of epilepsy are developed each year. Of these, 30% of patients were treated with ineffective medication, developing into a drug-incurable epilepsy that required surgery and other treatments. The most common type of epilepsy is temporal lobe epilepsy, while the common complication in temporal lobe epilepsy is hippocampal sclerosis, which often requires surgical removal. The incidence of inner temporal lobe epilepsy associated with hippocampal sclerosis is increasing, but its exact cause and specific pathogenesis are still unclear, so clarifying its pathogenesis will contribute to the understanding of temporal lobe epilepsy and the improvement of surgical procedures. This study is intended to get single-cell transcriptome as well as spatial transcriptome data of temporal lobe and hippocampus samples. By studying gene expression change associated with epilepsy and hippocampus sclerosis, we intended to find possible prognostic-related molecules and to deepen understanding of pathological changes in epilepsy at the molecular level.

Condition or Disease Intervention/Treatment Phase
  • Other: RNA sequencing

Study Design

Study Type:
Observational
Anticipated Enrollment :
10 participants
Observational Model:
Case-Control
Time Perspective:
Retrospective
Official Title:
Gene Expression Changes of Brain Tissue in Human Temporal Lobe Epilepsy With Hippocampal Sclerosis
Actual Study Start Date :
Apr 19, 2021
Anticipated Primary Completion Date :
Sep 1, 2022
Anticipated Study Completion Date :
Dec 1, 2022

Arms and Interventions

Arm Intervention/Treatment
epilepsy with HS

Other: RNA sequencing
Samples in both groups will be sequenced by single-cell sequencing and spatial transcription sequencing.

epilepsy without HS

Other: RNA sequencing
Samples in both groups will be sequenced by single-cell sequencing and spatial transcription sequencing.

Outcome Measures

Primary Outcome Measures

  1. RNA sequencing data [up to 24 weeks]

    Single-cell sequencing and spatial transcription sequencing data of samples.

Eligibility Criteria

Criteria

Ages Eligible for Study:
N/A and Older
Sexes Eligible for Study:
All
Inclusion Criteria:
  • Patients confirmed in our hospital as having temporal lobe epilepsy and intending surgery to remove temporal lobe and hippocampal tissue;

  • Volunteer to participate in this clinical trial, comply with the requirements of this clinical trial and sign an informed consent form.

Exclusion Criteria:
  • Chronic diseases of the central nervous system other than temporal lobe epilepsy;

  • Other patients that are not suitable for this clinical trial determined by the researchers;

Contacts and Locations

Locations

Site City State Country Postal Code
1 2ndAffiliated Hospital, School of Medicine, Zhejiang University, China Hangzhou Zhejiang China

Sponsors and Collaborators

  • Second Affiliated Hospital, School of Medicine, Zhejiang University

Investigators

  • Study Chair: 2nd Affiliated Hospital, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.
Responsible Party:
Second Affiliated Hospital, School of Medicine, Zhejiang University
ClinicalTrials.gov Identifier:
NCT04917939
Other Study ID Numbers:
  • 2021-0291
First Posted:
Jun 8, 2021
Last Update Posted:
Jun 8, 2021
Last Verified:
Jun 1, 2021
Studies a U.S. FDA-regulated Drug Product:
No
Studies a U.S. FDA-regulated Device Product:
No
Keywords provided by Second Affiliated Hospital, School of Medicine, Zhejiang University
Additional relevant MeSH terms:

Study Results

No Results Posted as of Jun 8, 2021