Genetically Modified T-Cell Therapy in Treating Patients With Advanced ROR1+ Malignancies

Sponsor

Fred Hutchinson Cancer Center (Other)

Overall Status

Terminated

CT.gov ID

NCT02706392

Collaborator

National Cancer Institute (NCI) (NIH)

Enrollment

Location

Arm

66.4

Actual Duration (Months)

0.3

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

This phase I trial studies the side effects and best dose of genetically modified T-cell therapy in treating patients with receptor tyrosine kinase-like orphan receptor 1 positive (ROR1+) chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), acute lymphoblastic leukemia (ALL), stage IV non-small cell lung cancer (NSCLC), or triple negative breast cancer (TNBC) that has spread to other places in the body and usually cannot be cured or controlled with treatment (advanced). Genetically modified therapies, such as ROR1 specific chimeric antigen receptor (CAR) T-cells, are taken from a patient's blood, modified in the laboratory so they specifically may kill cancer cells with a protein called ROR1 on their surfaces, and safely given back to the patient after conventional therapy. The "genetically modified" T-cells have genes added in the laboratory to make them recognize ROR1.

Condition or Disease	Intervention/Treatment	Phase
Hematopoietic and Lymphoid Cell Neoplasm Malignant Solid Neoplasm Metastatic Lung Non-Small Cell Carcinoma Metastatic Triple-Negative Breast Carcinoma Recurrent Acute Lymphoblastic Leukemia Recurrent Mantle Cell Lymphoma Refractory Chronic Lymphocytic Leukemia Stage III Lung Non-Small Cell Cancer AJCC v7 Stage IIIA Lung Non-Small Cell Cancer AJCC v7 Stage IIIB Lung Non-Small Cell Cancer AJCC v7 Stage IV Breast Cancer AJCC v6 and v7 Stage IV Lung Non-Small Cell Cancer AJCC v7 Unresectable Lung Non-Small Cell Carcinoma	Other: Laboratory Biomarker Analysis Biological: ROR1 CAR-specific Autologous T-Lymphocytes	Phase 1

Detailed Description

PRIMARY OBJECTIVE:

To evaluate the safety of adoptive T cell therapy using ex vivo expanded autologous cluster of differentiation (CD)8+ and CD4+ ROR1 CAR-T cells for patients with advanced ROR1+ hematologic (Cohort A) and epithelial (Cohort B) malignancies.

SECONDARY OBJECTIVES:

To determine duration of in vivo persistence of adoptively transferred T cells, and the phenotype of persisting T cells.
To determine trafficking of adoptively transferred T cells traffic to the bone marrow or other tumor site and function in vivo.
To determine preliminary antitumor activity of the adoptive transfer of ROR1 CAR-T cells in patients with measurable tumor burden prior to T cell transfer.

OUTLINE: This is a dose escalation study of ROR1 CAR-specific autologous T-lymphocytes.

Patients receive chemotherapy comprising fludarabine phosphate and cyclophosphamide as determined by the referring physician in consultation with the protocol principal investigator (PI). Beginning within 36-96 hours after completion of lymphodepleting chemotherapy, patients receive ROR1 CAR-specific autologous T-lymphocytes intravenously (IV) over 20-30 minutes. Patients may receive a second infusion of ROR1 CAR-specific autologous T-lymphocytes with or without additional cytoreductive therapy at the same (for those that received the highest cell dose) or up to the next highest dose level and there is persistent disease, there were no toxicities attributed to the first infusion, and the patient is at least 21 days from the first T cell infusion.

After completion of study treatment, patients are followed up for at least 15 years.

Study Design

Study Type:

Interventional

Actual Enrollment :

21 participants

Allocation:

N/A

Intervention Model:

Single Group Assignment

Masking:

None (Open Label)

Primary Purpose:

Treatment

Official Title:

Phase I Study of Adoptive Immunotherapy for Advanced ROR1+ Malignancies With Defined Subsets of Autologous T Cells Engineered to Express a ROR1-Specific Chimeric Antigen Receptor

Actual Study Start Date :

Mar 16, 2016

Actual Primary Completion Date :

May 17, 2021

Actual Study Completion Date :

Sep 28, 2021

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Treatment (ROR1 CAR-specific autologous T-lymphocytes) Patients receive chemotherapy comprising fludarabine phosphate and cyclophosphamide as determined by the referring physician in consultation with the protocol PI. Beginning within 36-96 hours after completion of lymphodepleting chemotherapy, patients receive ROR1 CAR-specific autologous T-lymphocytes IV over 20-30 minutes. Patients may receive a second infusion of ROR1 CAR-specific autologous T-lymphocytes with or without additional cytoreductive therapy at the same (for those that received the highest cell dose) or up to the next highest dose level and there is persistent disease, there were no toxicities attributed to the first infusion, and the patient is at least 21 days from the first T cell infusion.	Other: Laboratory Biomarker Analysis Correlative studies Biological: ROR1 CAR-specific Autologous T-Lymphocytes Given IV Other Names: Autologous ROR1-CAR-T Cells Chimeric Anti-ROR1 T-cell Receptor-expressing Autologous T-lymphocytes ROR1-CAR-T

Arm

Intervention/Treatment

Experimental: Treatment (ROR1 CAR-specific autologous T-lymphocytes)

Patients receive chemotherapy comprising fludarabine phosphate and cyclophosphamide as determined by the referring physician in consultation with the protocol PI. Beginning within 36-96 hours after completion of lymphodepleting chemotherapy, patients receive ROR1 CAR-specific autologous T-lymphocytes IV over 20-30 minutes. Patients may receive a second infusion of ROR1 CAR-specific autologous T-lymphocytes with or without additional cytoreductive therapy at the same (for those that received the highest cell dose) or up to the next highest dose level and there is persistent disease, there were no toxicities attributed to the first infusion, and the patient is at least 21 days from the first T cell infusion.

Other: Laboratory Biomarker Analysis

Correlative studies

Biological: ROR1 CAR-specific Autologous T-Lymphocytes

Given IV

Other Names:

Autologous ROR1-CAR-T Cells

Chimeric Anti-ROR1 T-cell Receptor-expressing Autologous T-lymphocytes

ROR1-CAR-T

Outcome Measures

Primary Outcome Measures

Incidence of adverse events [Within 35 days of receptor tyrosine kinase-like orphan receptor 1 positive chimeric antigen receptor-T cell infusion]
Will be graded according to Common Terminology Criteria for Adverse Events.

Secondary Outcome Measures

Duration of persistence of adoptively transferred receptor tyrosine kinase-like orphan receptor 1 positive chimeric antigen receptor-T cells [Up to 365 days after the T cell infusion]
Data should be collected for persistence of transferred T cells and descriptive statistics will be used to summarize the changes from baseline where possible.
Identification of sites where receptor tyrosine kinase-like orphan receptor 1 positive chimeric antigen receptor-T cells migrate [Up to 1 year]
Samples of bone marrow, blood and other tissues (e.g. cerebral spinal fluid, tumor, thoracentesis fluid) will be collected from patients as clinically indicated. Receptor tyrosine kinase-like orphan receptor 1 positive chimeric antigen receptor-T cells and their frequency/persistence will be detected by flow cytometry, polymerase chain reaction, and immunohistochemistry as appropriate
Objective response rate of complete remission and partial remission [Up to 1 year]
Data should be collected for efficacy of transferred T cells and descriptive statistics will be used to summarize the changes from baseline where possible. For those patients with measurable disease at the time T cell therapy commences, responses will be evaluated using standard response criteria based on computed tomography or positron emission tomography imaging and histologic analysis of bone marrow or other tissue samples.
Progression free survival [Up to 1 year]
Data should be collected for efficacy of transferred T cells and descriptive statistics will be used to summarize the changes from baseline where possible.
Overall survival [Up to 1 year]
Data should be collected for efficacy of transferred T cells and descriptive statistics will be used to summarize the changes from baseline where possible.

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years and Older

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria:

INCLUSION CRITERIA FOR PATIENTS WITH CLL, MCL OR ALL (COHORT A)

CLL who are beyond first remission and who have failed combination chemoimmunotherapy with regimens containing a purine analogue and anti-CD20 antibody, or who have failed tyrosine kinase or phosphatidylinositol 3 (PI3) kinase inhibitors, or who were not eligible for or declined such therapy; patients with fludarabine refractory disease are eligible
Mantle cell lymphoma patients who are beyond first remission and previously treated with chemoimmunotherapy; patients who have relapsed following autologous hematopoietic cell transplant (HCT) are eligible
ALL patients who have relapsed or have residual disease following treatment with curative intent; ALL patients must have ROR1 expressed on > 90% of the leukemia blasts to be eligible
Confirmation of diagnosis by internal pathology review of initial or subsequent biopsy or other pathologic material at the Fred Hutchinson Cancer Research Center (FHCRC)/Seattle Cancer Care Alliance (SCCA)
Evidence of ROR1 expression by immunohistochemistry or flow cytometry on any prior or current tumor specimen
Karnofsky performance status >= 70%
Negative pregnancy test for women of childbearing potential; subjects of childbearing potential are those who have not been surgically sterilized or have not been free from menses for > 1 year
Fertile male and female patients must be willing to use a contraceptive method before, during, and for at least two months after the T cell infusion
Ability to understand and provide informed consent

INCLUSION CRITERIA FOR PATIENTS WITH NSCLC OR TNBC (COHORT B):

Patients with non-small cell lung cancer that is metastatic or inoperable and who have been treated with at least one line of prior therapy or declined conventional therapy
Patients with known epidermal growth factor receptor (EGFR) or anaplastic lymphoma kinase (ALK) mutations must have been treated on at least one line of molecularly targeted therapy (e.g., erlotinib, crizotinib)
Patients must have measurable disease by at least one of the criteria below:
Extra skeletal disease that can be accurately measured in at least one dimension as >= 10 mm with conventional computed tomography (CT) techniques as defined by Response Evaluation Criteria in Solid Tumors (RECIST) 1.1
Skeletal or bone-only disease measurable by fludeoxyglucose F 18 (FDG) positron emission tomography (PET) imaging
ROR1 expression in > 20% of the primary tumor or metastasis by immunohistochemistry (IHC)
Karnofsky performance status of >= 70%
Patients must be off chemotherapy for a minimum of 3 weeks prior to start of treatment; targeted therapies must be stopped at least 3 days prior to start of lymphodepletion
Negative pregnancy test for women of childbearing potential; subjects of childbearing potential are those who have not been surgically sterilized or have not been free from menses for > 1 year
Fertile male and female patients must be willing to use a contraceptive method before, during and for at least two months after the T cell infusion
Ability to understand and provide informed consent

INCLUSION CRITERIA FOR TNBC:

Histologically confirmed diagnosis of metastatic TNBC; i.e. breast cancer that is estrogen receptor (ER) negative (=< 10%), progesterone receptor (PR) negative (=< 10%), and human epidermal growth factor receptor 2 (HER2) negative (0 or 1+ by immunohistochemistry or negative for gene amplification by fluorescence in situ hybridization [FISH])
Patients must have measurable disease by at least one of the criteria below:
Extra skeletal disease that can be accurately measured in at least one dimension as >= 10 mm with conventional CT techniques as defined by RECIST 1.1
Skeletal or bone-only disease measurable by FDG PET imaging
Patients must have received standard adjuvant, neoadjuvant, and/or metastatic chemotherapy per National Comprehensive Cancer Network (NCCN) or institutional practice; no maximum on number of prior systemic treatment regimens
Patients may receive agents to protect against skeletal related complications such as zoledronic acid or denosumab
ROR1 expression in > 20% of the primary tumor or metastasis by IHC
Karnofsky performance status of >= 70%
Patients must be off chemotherapy for a minimum of 3 weeks prior to planned leukapheresis
Negative pregnancy test for women of childbearing potential; subjects of childbearing potential are those who have not been surgically sterilized or have not been free from menses for > 1 year
Fertile male and female patients must be willing to use a contraceptive method before, during and for at least two months after the T cell infusion
Ability to understand and provide informed consent

Exclusion Criteria:

EXCLUSION CRITERIA FOR PATIENTS WITH CLL, MCL, OR ALL (COHORT A)

Treatment with other investigational agent(s) within 30 days of planned lymphodepletion
Patients requiring ongoing daily corticosteroid therapy at a dose of > 15 mg of prednisone per day (or equivalent); pulsed corticosteroid use for disease control is acceptable
Active autoimmune disease requiring immunosuppressive therapy
Serum creatinine > 2.5 mg/dL
Serum glutamic oxaloacetic transaminase (SGOT) > 5 x upper limit of normal
Bilirubin > 3.0 mg/dL
Patients with clinically significant pulmonary dysfunction, as determined by medical history and physical exam should undergo pulmonary function testing; those with a forced expiratory volume in 1 second (FEV1) of =< 65% or diffusion capacity of the lung for carbon monoxide (DLCO) (corrected) < 40% will be excluded
Significant cardiovascular abnormalities as defined by any one of the following: congestive heart failure, clinically significant hypotension, symptomatic coronary artery disease, or a documented ejection fraction of < 45%; any patient with an ejection fraction (EF) of 45-49% must receive clearance by a cardiologist to be eligible for the trial
Patients who are human immunodeficiency virus (HIV) seropositive
Uncontrolled active infection (bacterial, viral, fungal, mycobacterial) not responding to treatment with intravenous antibiotics, antiviral or antifungal agents, or long-term treatment with oral agents
Women who are breast-feeding
Patients who have contraindication to cyclophosphamide chemotherapy
Known additional malignancy that is progressing or requires active treatment; exceptions include basal cell carcinoma of the skin, squamous cell carcinoma of the skin, or in situ cervical cancer that has undergone potentially curative therapy
Untreated central nervous system (CNS) metastases and/or carcinomatous meningitis; subjects with previously treated brain metastases may participate provided they are stable (without evidence of progression by imaging for at least four weeks prior to enrollment and any neurologic symptoms have returned to baseline), have no evidence of new or enlarging brain metastases

EXCLUSION CRITERIA FOR PATIENTS WITH NSCLC OR TNBC (COHORT B):

Absolute neutrophil count (ANC) < 1000/mm^3
Hemoglobin (Hgb) < 9 mg/dl (transfusion permitted to achieve this)
Platelet count < 75,000/mm^3
Treatment with other investigational agent(s) within 30 days of planned lymphodepletion
Patients requiring ongoing daily corticosteroid therapy at a dose of > 15 mg of prednisone per day (or equivalent); pulsed corticosteroid use for disease control is acceptable
Active autoimmune disease requiring immunosuppressive therapy
Serum creatinine > 2.5 mg/dL
SGOT > 5 x upper limit of normal
Bilirubin > 3.0 mg/dL
Patients with clinically significant pulmonary dysfunction, as determined by medical history and physical exam should undergo pulmonary function testing; those with an FEV1 of =< 65% or DLCO (corrected) < 40% will be excluded
Significant cardiovascular abnormalities as defined by any one of the following: congestive heart failure, clinically significant hypotension, symptomatic coronary artery disease, or a documented ejection fraction of < 45%; any patient with an EF of 45-49% must receive clearance by a cardiologist to be eligible for the trial
Patients who are HIV seropositive
Uncontrolled active infection (bacterial, viral, fungal, mycobacterial) not responding to treatment with intravenous antibiotics, antiviral or antifungal agents, or long-term treatment with oral agents
Women who are breastfeeding
Patients who have contraindication to cyclophosphamide chemotherapy
Known additional malignancy that is progressing or requires active treatment; exceptions include basal cell carcinoma of the skin, squamous cell carcinoma of the skin, or in situ cervical cancer that has undergone potentially curative therapy
Untreated central nervous system (CNS) metastases and/or carcinomatous meningitis; subjects with previously treated brain metastases may participate provided they are stable (without evidence of progression by imaging for at least four weeks prior to enrollment and any neurologic symptoms have returned to baseline), have no evidence of new or enlarging brain metastases

EXCLUSION CRITERIA FOR TNBC:

ANC < 1000/mm^3
Hgb < 9 mg/dl (transfusion permitted to achieve this)
Platelet count < 75,000/mm^3
Treatment with other investigational agent(s) within 30 days of planned lymphodepletion
Patients requiring ongoing daily corticosteroid therapy at a dose of > 15 mg of prednisone per day (or equivalent); pulsed corticosteroid use for disease control is acceptable
Active autoimmune disease requiring immunosuppressive therapy
Serum creatinine > 2.5 mg/dL
SGOT > 5 x upper limit of normal
Bilirubin > 3.0 mg/dL
Patients with clinically significant pulmonary dysfunction, as determined by medical history and physical exam should undergo pulmonary function testing; those with an FEV1 of =< 65% or DLCO (corrected) < 40% will be excluded
Significant cardiovascular abnormalities as defined by any one of the following: congestive heart failure, clinically significant hypotension, symptomatic coronary artery disease or a documented ejection fraction of < 45%; any patient with an EF of 45-49% must receive clearance by a cardiologist to be eligible for the trial
Patients who are HIV seropositive
Uncontrolled active infection (bacterial, viral, fungal, mycobacterial) not responding to treatment with intravenous antibiotics, antiviral or antifungal agents, or long-term treatment with oral agents
Breast-feeding women
Patients who have contraindication to cyclophosphamide chemotherapy
Has a known additional malignancy that is progressing or requires active treatment; exceptions include basal cell carcinoma of the skin, squamous cell carcinoma of the skin, or in situ cervical cancer that has undergone potentially curative therapy
Untreated central nervous system (CNS) metastases and/or carcinomatous meningitis; subjects with previously treated brain metastases may participate provided they are stable (without evidence of progression by imaging for at least four weeks prior to enrollment and any neurologic symptoms have returned to baseline) and have no evidence of new or enlarging brain metastases