TIBIOP-LMC: Innate T Cells and TKI Discontinuation
Study Details
Study Description
Brief Summary
After more than a decade of treating chronic myeloid leukemia (CML) with tyrosine kinase inhibitors (TKI), the discontinuation of treatment represents the expected new revolution. The investigators has recently discovered a new innate CD8+ T population in healthy subjects, the Eomes+ KIR+ CD8+ T population, with anti-tumor properties. Remarkably, these cells are numerically and functionally deficient in patients at diagnosis and then restored in patients in major molecular remission (MMR) on TKI. Our work performed in a retrospective pilot study interestingly shows a very significant increase in the proportion of CD8+ Eomes+ KIR+ T cells within total T cells in patients with prolonged success in stopping their ITK (≥ 2 years).Thus, the investigators postulate that CD8+ Eomes+ KIR+ T cells are a predictive signature of TKI arrest success in CML. The investigators will rely on a prospective translational study of this cell contingent during treatment cessation.
Condition or Disease | Intervention/Treatment | Phase |
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Detailed Description
To perform this research, the investigators have started a prospective translational study to collect samples in CML patients with successful versus patients who have failed TKI therapy discontinuation. The investigators plan to study functional and phenotypic characteristics of innate T cells. The investigators also plan to evaluate in vitro whether immune check points inhibitors could help to restore the innate T lymphocytes population.
Study Design
Outcome Measures
Primary Outcome Measures
- Compare the quantitative characteristics of the CD8+ Eomes+ KIR+ T cells between patients in failure versus those in success after discontinuation of TKI treatment [Day 0 (day of discontinuing treatment)]
Proportion of the CD8+ Eomes+ KIR+ T cells among CD8+ T cells between patients in failure versus those in success after discontinuation of TKI treatment
- Compare phenotypic characteristics of the CD8+ Eomes+ KIR+ T cells between patients in failure versus those in success after discontinuation of TKI treatment [Day 0 (day of discontinuing treatment)]
Phenotypic markers expression : CD49d, CD57, CD45RA et CCR7, CD25 et HLA-DR among total CD8+ T cells
- Compare the functionnality of the CD8+ Eomes+ KIR+ T cells between patients in failure versus those in success after discontinuation of TKI treatment [Day 0 (day of discontinuing treatment)]
Functionality of LT CD8+ Eome+ KIR+ : expression of perforin and IFNgamma
Eligibility Criteria
Criteria
Inclusion Criteria:
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Age > 18 years old
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Diagnosis of CML-PC according to the criteria of the European Leukemia Net (Baccarani et al, 2013)
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Patients treated with TKI for at least 3 years (imatinib, nilotinib, dasatinib, bosutinib, ponatinib)
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Patients meeting the criteria of the French STIM study: deep molecular response of MR4.5 type (threshold of 0.0032%) or MR5 type (threshold of 0.001%) for at least 2 years,
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Free subject, without guardianship or curatorship or subordination
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Patients benefiting from a Social Security system or benefiting from it through a third party
Exclusion Criteria:
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Refusal to participate in the research
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Patients not benefiting from a Social Security scheme or not benefiting from it through a third party
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Persons benefiting from enhanced protection, namely minors, persons deprived of their liberty by a judicial or administrative decision, persons staying in a health or social institution, adults under legal protection, and finally patients in emergencies
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Patient having stopped treatment for another reason than: deep molecular response of MR4.5 (threshold of 0.0032%) or MR5 (threshold of 0.001%) for at least 2 years
Translated with www.DeepL.com/Translator (free version)
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- Poitiers University Hospital
Investigators
- Study Director: Emilie CAYSSIALS, Poitiers University Hospital
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- TIBIOP-LMC