MitoWomen: Mitochondrial Function in Circulating Cells and Muscle Tissue
Study Details
Study Description
Brief Summary
The main goal of this project is to determine whether mitochondrial function in circulating cells is related to that measured in the muscle fibers of the same subjects.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
|
Detailed Description
Data from human and animal models strongly suggests that reduced tissue mitochondrial function can be an early marker for detection of dysfunctional metabolism. It is unclear if tissue mitochondrial dysfunction can be detected using less invasive methodology. In this project, the investigators plan to study mitochondrial bioenergetics in circulating cells and paired muscle biopsies in two metabolic disparate groups of women; healthy lean vs. overweight/obese with/without insulin resistant women of similar age. Women of similar age that are either healthy lean or overweight/obese with/without insulin resistance will be recruited to participate in one study that includes a blood draw and a muscle biopsy. Mitochondrial function in circulating blood cells and muscle tissue will be determined. The association between circulating blood cells and muscle tissue mitochondria respiration will be determined for the two groups combined, and differences between groups will also be investigated.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Healthy lean Healthy lean women, 25-35 years |
|
| Overweight/obese with/without IR Overweight/obese with/without insulin resistant women, 25-35 years |
Outcome Measures
Primary Outcome Measures
- Mitochondrial Function in Circulating Cells and Muscle Tissue in Young Women [During the visit, approximately 0.5 days]
Skeletal muscle (m.vastus lateralis) and peripheral blood mononuclear cell (PBMC) mitochondrial respiration were determined in response to substrate inhibitor coupling protocols using high resolution respirometry. From this, mitochondrial respiration in leak and coupled states, and reserve respiratory capacity were determined in mitochondria from skeletal muscle and from PBMCs. It was determined whether mitochondrial function in circulating cells is related to that measured in the muscle tissue of the same subjects.
Eligibility Criteria
Criteria
Inclusion Criteria:
-
Women
-
25-35 years of age
-
Either healthy lean (BMI<25) or insulin resistant and obese (BMI >30)
Exclusion Criteria:
-
Medication that can affect study outcomes, as determined by the principal investigator, including anti-diabetic medication
-
Chronic diseases, acute inflammation, chronic renal failure (glomerular filtration rate <45), or heart problems
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Arkansas Children's Nutrition Center | Little Rock | Arkansas | United States | 72202 |
| 2 | UAMS | Little Rock | Arkansas | United States | 72202 |
Sponsors and Collaborators
- University of Arkansas
- Arkansas Children's Hospital Research Institute
Investigators
- Principal Investigator: Elisabet Borsheim, PhD, Arkansas Children's Nutrition Center
Study Documents (Full-Text)
More Information
Publications
None provided.- 205139
Study Results
Participant Flow
| Recruitment Details | |
|---|---|
| Pre-assignment Detail |
| Arm/Group Title | Healthy Lean | Overweight/Obese +/- IR |
|---|---|---|
| Arm/Group Description | Healthy lean women, 25-35 years | Overweight/obese and/or insulin resistant women, 25-35 years |
| Period Title: Overall Study | ||
| STARTED | 17 | 18 |
| COMPLETED | 16 | 18 |
| NOT COMPLETED | 1 | 0 |
Baseline Characteristics
| Arm/Group Title | Healthy Lean | Overweight/Obese With/Without IR | Total |
|---|---|---|---|
| Arm/Group Description | Healthy lean women, 25-35 years | Overweight/obese with/without insulin resistant women, 25-35 years | Total of all reporting groups |
| Overall Participants | 17 | 18 | 35 |
| Age, Customized (Count of Participants) | |||
| 25-35 years |
17
100%
|
18
100%
|
35
100%
|
| Sex/Gender, Customized (Count of Participants) | |||
| Female |
17
100%
|
18
100%
|
35
100%
|
| Race (NIH/OMB) (Count of Participants) | |||
| American Indian or Alaska Native |
0
0%
|
0
0%
|
0
0%
|
| Asian |
0
0%
|
0
0%
|
0
0%
|
| Native Hawaiian or Other Pacific Islander |
0
0%
|
0
0%
|
0
0%
|
| Black or African American |
4
23.5%
|
5
27.8%
|
9
25.7%
|
| White |
13
76.5%
|
12
66.7%
|
25
71.4%
|
| More than one race |
0
0%
|
0
0%
|
0
0%
|
| Unknown or Not Reported |
0
0%
|
1
5.6%
|
1
2.9%
|
| Region of Enrollment (participants) [Number] | |||
| United States |
17
100%
|
18
100%
|
35
100%
|
| BMI (kg/m^2) [Mean (Standard Deviation) ] | |||
| Mean (Standard Deviation) [kg/m^2] |
22.4
(1.4)
|
35.6
(7.0)
|
29.2
(8.4)
|
Outcome Measures
| Title | Mitochondrial Function in Circulating Cells and Muscle Tissue in Young Women |
|---|---|
| Description | Skeletal muscle (m.vastus lateralis) and peripheral blood mononuclear cell (PBMC) mitochondrial respiration were determined in response to substrate inhibitor coupling protocols using high resolution respirometry. From this, mitochondrial respiration in leak and coupled states, and reserve respiratory capacity were determined in mitochondria from skeletal muscle and from PBMCs. It was determined whether mitochondrial function in circulating cells is related to that measured in the muscle tissue of the same subjects. |
| Time Frame | During the visit, approximately 0.5 days |
Outcome Measure Data
| Analysis Population Description |
|---|
| Out of the 35 enrolled, 32 participants had all samples (paired blood and muscle samples). Thus, they were their own controls, and for the main question about correlation between circulating cells and muscle mitochondrial respiration, samples were all analyzed together. This enabled comparison of circulating cells/muscle mitochondria respiration along a spectrum of BMIs and insulin resistance. |
| Arm/Group Title | Healthy Lean | Overweight/Obese +/- IR |
|---|---|---|
| Arm/Group Description | Healthy lean women, 25-35 years | Overweight/obese and/or insulin resistant women, 25-35 years |
| Measure Participants | 16 | 16 |
| PBMC ATP linked respiration |
26.48
(2.54)
|
30.46
(2.44)
|
| PBMC max respiration |
120.04
(11.88)
|
140.43
(14.30)
|
Adverse Events
| Time Frame | Approximately 10 days following biopsy | |||
|---|---|---|---|---|
| Adverse Event Reporting Description | Cross-sectional study with no intervention. Moderate risk study with one blood sample and one muscle biopsy. | |||
| Arm/Group Title | Healthy Lean | Overweight/Obese +/- IR | ||
| Arm/Group Description | Healthy lean women, 25-35 years | Overweight/obese and/or insulin resistant women, 25-35 years | ||
All Cause Mortality |
||||
| Healthy Lean | Overweight/Obese +/- IR | |||
| Affected / at Risk (%) | # Events | Affected / at Risk (%) | # Events | |
| Total | 0/17 (0%) | 0/18 (0%) | ||
Serious Adverse Events |
||||
| Healthy Lean | Overweight/Obese +/- IR | |||
| Affected / at Risk (%) | # Events | Affected / at Risk (%) | # Events | |
| Total | 0/17 (0%) | 0/18 (0%) | ||
Other (Not Including Serious) Adverse Events |
||||
| Healthy Lean | Overweight/Obese +/- IR | |||
| Affected / at Risk (%) | # Events | Affected / at Risk (%) | # Events | |
| Total | 0/17 (0%) | 0/18 (0%) | ||
Limitations/Caveats
More Information
Certain Agreements
All Principal Investigators ARE employed by the organization sponsoring the study.
There is NOT an agreement between Principal Investigators and the Sponsor (or its agents) that restricts the PI's rights to discuss or publish trial results after the trial is completed.
Results Point of Contact
| Name/Title | Elisabet Borsheim, PhD |
|---|---|
| Organization | University of Arkansas for Medical Sciences; Arkansas Children's Research Institute |
| Phone | 501-364-3053 |
| EBorsheim@uams.edu |
- 205139