The Molecular Pathogenesis Study of Cataract
Study Details
Study Description
Brief Summary
To study the molecular pathogenesis of cataract and explore possible treatments,anterior lens capsules were collected from cataract patients
Condition or Disease | Intervention/Treatment | Phase |
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Detailed Description
Anterior lens capsules were collected from the patients with DC and ARC, and transparent crystals of cadaveric eyes. M6A epitranscriptomic microarray was performed to investigate the altered m6A modifications and determine the differentially expressed mRNAs. The potential role of dysregulated m6A modification levels was predicted through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Real-time polymerase chain reaction was performed to identify the dysregulated expression of RNA methyltransferases, demethylases, and readers.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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DC group Anterior lens capsule tissues from DC patients |
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ARC group Anterior lens capsule tissues from ARC patients |
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NC group anterior lens capsules collected from three age-matched transparent crystals of cadaveric eyes |
Outcome Measures
Primary Outcome Measures
- m6A modification of mRNAs [1 month]
Epitranscriptomic microarray analysis reveals the differential m6A modification of mRNAs in DC samples
- expression level of mRNAs [1 month]
the m6A microarray analyses provide data for mRNA expression levels
- levels of methylated mRNA [1 month]
in vitro validation of the diverse expression levels of methylated mRNA
- levels of RNA methyltransferase [1 month]
in vitro validation of the diverse expression levels of RNA methyltransferase
Eligibility Criteria
Criteria
Inclusion Criteria:
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diabetic cataract
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underwent cataract surgery
Exclusion Criteria:
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Combined with other eye diseases
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underwent other eye surgery
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
---|---|---|---|---|---|
1 | Eye & ENT Hospital of Fudan University | Shanghai | Shanghai | China | 200031 |
Sponsors and Collaborators
- Eye & ENT Hospital of Fudan University
- National Natural Science Foundation of China
Investigators
- Principal Investigator: Jin Yang, PhD, Eye & ENT Hospital
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- CMP