BUBBLE TEST: Implementation of a Non-invasive Version of the Imaging β-adrenergic-dependent Sweat Secretion Test
Study Details
Study Description
Brief Summary
The goal of this study is to implement a non-invasive version of the sweat secretion test based in visualization of sweat glands following β-adrenergic stimulation of sweat. Specifically, the trial will evaluate the relative response of sweat glands to β-adrenergic and cholinergic stimulation among participants with CF, heterozygous and matched control subjects
Condition or Disease | Intervention/Treatment | Phase |
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N/A |
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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Experimental: Patients with mucoviscidosis For patients with cystic fibrosis: clinically stable, all genotypes included. |
Diagnostic Test: Iontophoresis sessions in forearm of subjects with pilocarpine
The test with two iontophoresis sessions is performed in a forearm of subjects. Pilocarpine is applied during the first iontophoresis session to evoke cholinergic-induced sweat secretion. Atropine, isoprenaline and aminophylline are applied during the second iontophoresis session, to block the cholinergic component and to evoke β-adrenergic-induced sweat secretion.
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Experimental: Parents of patients with confirmed diagnosis of mucoviscidosis The parents are heterozygous subjects |
Diagnostic Test: Iontophoresis sessions in forearm of subjects with pilocarpine
The test with two iontophoresis sessions is performed in a forearm of subjects. Pilocarpine is applied during the first iontophoresis session to evoke cholinergic-induced sweat secretion. Atropine, isoprenaline and aminophylline are applied during the second iontophoresis session, to block the cholinergic component and to evoke β-adrenergic-induced sweat secretion.
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Active Comparator: Healthy volunteers
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Diagnostic Test: Iontophoresis sessions in forearm of subjects with pilocarpine
The test with two iontophoresis sessions is performed in a forearm of subjects. Pilocarpine is applied during the first iontophoresis session to evoke cholinergic-induced sweat secretion. Atropine, isoprenaline and aminophylline are applied during the second iontophoresis session, to block the cholinergic component and to evoke β-adrenergic-induced sweat secretion.
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Outcome Measures
Primary Outcome Measures
- Ratio between the β-adrenergic/cholinergic rate [up to 30 minutes]
The ratio between the β-adrenergic/cholinergic rate (nL/min) of droplets of sweat is calculated.
Eligibility Criteria
Criteria
Inclusion Criteria:
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For patients with cystic fibrosis: clinically stable, all genotypes included.
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For healthy volunteer and heterozygous subjects: 18 years and older.
Exclusion Criteria:
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Corticosteroids, anti-leukotriene therapy, unstable clinical status, <40% FEV1.
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Current pregnancy or breastfeeding.
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | Cliniques universitaires Saint-Luc | Brussel | Belgium | 1200 |
Sponsors and Collaborators
- Cliniques universitaires Saint-Luc- Université Catholique de Louvain
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 2015/04MAI/231