CoFa: Confounding Factors in Ocular Surface Microbiome Research
Study Details
Study Description
Brief Summary
To elucidate confounding factors in ocular surface microbiome research and to confirm the presence of a core ocular surface microbiome and its temporal stability.
Condition or Disease | Intervention/Treatment | Phase |
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N/A |
Detailed Description
The investigator will take a conjunctival sample of both eyes of a group of volunteers together with an extensive questionnaire. Furthermore, 50 volunteers will be sampled repeatedly during one year or two years.
Study Design
Outcome Measures
Primary Outcome Measures
- Richness and evenness of distribution of the microbial flora measured as operational taxonomic units on a single moment [20 minutes for samples and filling the questionnaires]
A conjunctival swab and extensive questionnaire will be taken to search for a core ocular surface microbiome and confounding factors
- Change in richness and evenness of distribution of the microbial flora, measured as operational taxonomic units, over time [20 minutes for the first sample and questionnaire, 5 minutes for all other visits]
A conjunctival swab will be taken on different points in time to investigate the temporal stability of the conjunctival microbial flora
Eligibility Criteria
Criteria
Inclusion Criteria:
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The subject is fluent in written and verbal Dutch
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The subject is capable of giving informed consent
Exclusion Criteria:
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Use of glaucoma drops
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Use of antibiotic drops < 1 month before sampling
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | University Hospitals Leuven, Department of Ophthalmology | Leuven | Belgium | 3000 |
Sponsors and Collaborators
- Universitaire Ziekenhuizen Leuven
Investigators
- Principal Investigator: Heleen Delbeke, MD, Universitaire Ziekenhuizen Leuven
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- S63155