Platelet Enzymatically Oxidized Phospholipids (eoxPL) Characterisation in a Healthy Cohort on and Off Aspirin
Study Details
Study Description
Brief Summary
Healthy volunteers (n = 28) were recruited from the workplace (14 male, 14 female; age range 20-50 years). Ethical approval, which included informed consent, was from Cardiff University, Schools of Medicine and Dentistry Research Ethics Committee (SMREC16/02). Following a 2-week period free from non-steroidal anti-inflammatory drugs (NSAIDs), peripheral blood was obtained and platelets isolated. Participants were commenced on aspirin 75 mg once daily for seven days and then provided a repeat blood sample. Following a 2-month period, participants were invited to repeat samples pre- and post-aspirin, and again a third time 2 months later.
Condition or Disease | Intervention/Treatment | Phase |
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N/A |
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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Experimental: Aspirin supplemented
|
Drug: Aspirin
1 week of aspirin supplementation prior to sampling
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No Intervention: Off aspirin
|
Outcome Measures
Primary Outcome Measures
- Platelet EoxPL differences in amounts (ng) as measured by liquid chromatography with tandem mass spectrometry [4 months]
Samples on and off aspirin will be quantified for the 47 most common eoxPL species (as described by Slatter et al, Cell Metabolism 23, 930-944, May 10, 2016) using established liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differences in amounts on and off aspirin will be quantified and tested for statistical significance.
Eligibility Criteria
Criteria
Inclusion Criteria:
-
two-week washout period from any non-steroidal anti-inflammatory drugs (NSAIDs) use.
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Ability to take aspirin 75 mg once daily for seven days and sampled thereafter, before stopping the aspirin.
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Willingness to repeat this process after two months and four months of the initial sampling date.
Exclusion Criteria:
- Recent NSAID use
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
---|---|---|---|---|---|
1 | Sir Geraint Evans Cardiovascular Research Building | Cardiff | United Kingdom | CF144XN |
Sponsors and Collaborators
- Cardiff University
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- SMREC16/02