Functional Studies of Novel Genes Mutated in Primary Ciliary Dyskinesia II: Genotype to Phenotype

Sponsor
University of North Carolina, Chapel Hill (Other)
Overall Status
Recruiting
CT.gov ID
NCT04901715
Collaborator
National Heart, Lung, and Blood Institute (NHLBI) (NIH)
30
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Study Details

Study Description

Brief Summary

The purpose of this study is to measure mucociliary clearance (MCC) in groups of subjects with the disease Primary Ciliary Dyskinesia (PCD) caused by mutations in different genes, and compare to healthy subjects. Some of these genes are associated with a milder clinical phenotype. This study seeks to determine if the milder phenotype is a result of mutations in a set of specific genes. The hypothesis is that subjects with PCD caused by mutations in the milder group will maintain a low, but significant rate of mucociliary clearance, while patients with mutations in genes in the more severe group will have a complete absence of mucociliary clearance. These studies will help inform future treatment strategies.

Condition or Disease Intervention/Treatment Phase
  • Drug: Albuterol
  • Diagnostic Test: Technetium99m - Sulfur Colloid (Tc99m-SC)
Early Phase 1

Detailed Description

Participants will undergo screening with basic physical exam and lung function testing at the start of the study. Participants will then inhale a radiolabeled substance and undergo medical imaging to measure the clearance of mucus in the airways. Albuterol will be administered after the first imaging is completed. Lung function testing will be repeated. Finally, medical imaging will be repeated two more times to further look at clearance of mucus in the lungs. The study will be completed in one day and will last about 6 hours.

Study Design

Study Type:
Interventional
Anticipated Enrollment :
30 participants
Allocation:
Non-Randomized
Intervention Model:
Parallel Assignment
Masking:
None (Open Label)
Primary Purpose:
Basic Science
Official Title:
Functional Studies of Novel Genes Mutated in Primary Ciliary Dyskinesia II: Genotype to Phenotype
Actual Study Start Date :
Jun 10, 2021
Anticipated Primary Completion Date :
Apr 30, 2024
Anticipated Study Completion Date :
Apr 30, 2024

Arms and Interventions

Arm Intervention/Treatment
Active Comparator: Genotypes associated mild phenotype

Subjects with 2 confirmed mutations in RSPH1, Radial Spoke Head Component 9 (RSPH9), Radial Spoke Head Component 4A (RSPH4a), or Dynein Axonemal Heavy Chain 11 (DNAH11). This group may also include subjects with mutations in newly identified genes that are associated with a milder clinical phenotype.

Drug: Albuterol
Albuterol HFA Metered Dose Inhaler (90mcg/puff). Subjects to use 4 puffs one time.
Other Names:
  • ProAir HFA (hydrofluoroalkane) Inhaler
  • Ventolin HFA (hydrofluoroalkane) Inhaler
  • Diagnostic Test: Technetium99m - Sulfur Colloid (Tc99m-SC)
    Aerosolized radiolabeled Tc99m-sulfur colloid will be delivered using a modified Pari-LC Star nebulizer. The activity of Tc99m-SC loaded in the nebulizer will be adjusted to provide an estimated 40 uCi deposited in the lung for the MCC/CC scan. Patients between the age 12-18 years old will receive ¾ of the adult dose to account for the smaller lung volume.

    Active Comparator: Genotypes associated with severe phenotype

    Subjects with 2 confirmed mutations in DNAH5, Dynein Axonemal Intermediate Chain 1 (DNAI1), Coiled-Coil Domain Containing 39 (CCDC39), or Coiled-Coil Domain Containing 40 (CCDC40). This group may also include subjects with mutations in newly identified genes that are associated with a more severe clinical phenotype.

    Drug: Albuterol
    Albuterol HFA Metered Dose Inhaler (90mcg/puff). Subjects to use 4 puffs one time.
    Other Names:
  • ProAir HFA (hydrofluoroalkane) Inhaler
  • Ventolin HFA (hydrofluoroalkane) Inhaler
  • Diagnostic Test: Technetium99m - Sulfur Colloid (Tc99m-SC)
    Aerosolized radiolabeled Tc99m-sulfur colloid will be delivered using a modified Pari-LC Star nebulizer. The activity of Tc99m-SC loaded in the nebulizer will be adjusted to provide an estimated 40 uCi deposited in the lung for the MCC/CC scan. Patients between the age 12-18 years old will receive ¾ of the adult dose to account for the smaller lung volume.

    Active Comparator: Healthy Control

    Healthy subjects with no pre-existing lung disease.

    Drug: Albuterol
    Albuterol HFA Metered Dose Inhaler (90mcg/puff). Subjects to use 4 puffs one time.
    Other Names:
  • ProAir HFA (hydrofluoroalkane) Inhaler
  • Ventolin HFA (hydrofluoroalkane) Inhaler
  • Diagnostic Test: Technetium99m - Sulfur Colloid (Tc99m-SC)
    Aerosolized radiolabeled Tc99m-sulfur colloid will be delivered using a modified Pari-LC Star nebulizer. The activity of Tc99m-SC loaded in the nebulizer will be adjusted to provide an estimated 40 uCi deposited in the lung for the MCC/CC scan. Patients between the age 12-18 years old will receive ¾ of the adult dose to account for the smaller lung volume.

    Outcome Measures

    Primary Outcome Measures

    1. Baseline MCC (Ave60Clr; average clearance over 60 minutes) [60 minutes]

      The average percent clearance in subjects with PCD caused by mutations associated with mild and severe clinical phenotypes. Prior to each MCC study, a transmission Co57 scan will be performed to define the lung boundaries, to assign regions of interest, and to normalize these regions for lung volume differences. Radiolabeled Tc99m-sulfur colloid will be delivered using a modified Pari-LC Star nebulizer. The subject will then (within a minute of final inhalation maneuver) be seated in front of a large-field-of-view gamma camera to begin acquiring consecutive 2 minute images. The first two-2-minute images will provide initial, time zero activity (i.e. 100% retention) followed by the same imaging at the start of every 10-minute period until 1 hour has passed to assess baseline MCC. Clearance will be determined by measuring the decrease in radiolabeled Tc99m-sulfur colloid in the lungs over time.

    Secondary Outcome Measures

    1. Change in MCC (Ave120Clr-Ave60Clr;average clearance between 60 and 120 minutes) [120 minutes]

      The change in average percent clearance after the administration of albuterol in subjects with PCD caused by mutations associated with mild and severe clinical phenotypes PCD patients with mild disease compared to PCD patients with more severe disease. A transmission Cobalt57 scan will be performed to define the lung boundaries. Radiolabeled Tc99m-sulfur colloid (Technetium99m) will be delivered using a modified nebulizer. The subject will be seated in front of a large-field-of-view gamma camera to begin consecutive 2 minute images. The first two-2-minute images will provide initial, time zero activity followed by the same imaging at every 10-minute period until 1 hour. Subjects will then inhale 4 puffs of albuterol from the Metered Dose Inhaler (MDI) and consecutive 2 minute imaging continues for the next hour to assess the effect of albuterol on MCC. Clearance will be determined by measuring the decrease in radiolabeled Tc99m-sulfur colloid in the lungs over time.

    Other Outcome Measures

    1. Change in cough clearance (Ave150Clr-Ave120Clr; average clearance between 120 and 150 minutes) [150 minutes]

      The change in average percent of cough clearance in subjects with PCD caused by mutations associated with mild and severe clinical phenotypes PCD patients with mild disease compared to PCD patients with more severe disease. After completion of baseline MCC measurement and post-Albuterol MCC measurement. Subjects will cough a total of 30 times over a 30-minute period to assess cough clearance by gamma imaging over that period. Clearance will be determined by measuring the decrease in radiolabeled Tc99m-sulfur colloid in the lungs over time.

    Eligibility Criteria

    Criteria

    Ages Eligible for Study:
    12 Years to 90 Years
    Sexes Eligible for Study:
    All
    Accepts Healthy Volunteers:
    Yes

    Inclusion Criteria for PCD Patients

    • Confirmed PCD diagnosis with identified genetic mutations

    • Negative pregnancy test for females who are not s/p hysterectomy with oophorectomy

    • Forced Expiratory Volume (FEV1) of at least 30 percent of predicted

    Inclusion Criteria for Healthy Controls:
    • Age ≥ 18 years old

    • Subjects must have an FVC, FEV1 and FVC/FEV1 of at least 80% of predicted. Subjects who fall out of the normal range will be offered a copy of the test to share with their personal physician.

    • No pre-existing lung disease (asthma, cystic fibrosis, etc.).

    • Negative pregnancy test for females who are not s/p hysterectomy with oophorectomy.

    Exclusion Criteria:
    • Any chronic medical condition considered by the PI as a contraindication to the exposure study including significant cardiovascular disease, diabetes, chronic renal disease, chronic thyroid disease, immunodeficiency, history of tuberculosis

    • Any acute infection requiring antibiotics within 4 weeks of study.

    • Mental illness or history of drug or alcohol abuse that, in the opinion of the investigator, would interfere with the participant's ability to comply with study requirements.

    • Medications which may impact the results of the study treatment, or may interfere with any other medications potentially used in the study (to include steroids, beta antagonists, non-steroidal anti-inflammatory agents)

    • Active smoking to include e-cigarettes within 1 year of the study, or lifetime of > 10 pack years of smoking

    • History of vaping or current vaping.

    • Viral upper respiratory tract infection within 4 weeks of challenge.

    • Radiation exposure history in the past year which would be outside the safe levels

    • Pregnant or lactating women will also be excluded since the risks associated with radiation are unknown and cannot be justified

    • Use of the following medications:

    1. Use of beta blocking medications

    2. Receipt of LAIV (Live Attenuated Influenza Vaccine), also known as FluMist , within the prior 30 days, or any vaccine within the prior 5 days

    3. Multivitamins, Vitamin C or E or herbal medications in the 4 days prior to the treatment visit

    4. Non-steroidal anti-inflammatory drugs in the 4 days prior to the treatment visit.

    • Allergy/sensitivity to study drugs or their formulations:

    • Known Immunoglobulin E (IgE) mediated hypersensitivity to albuterol

    • Physical/laboratory indications:

    1. Temperature > 37.8 degrees Celsius (C)

    2. Subjects >15 years- Systolic BP >150 mm hg or < 90 mm Hg or diastolic BP> 90 mm Hg or < 50 and Subjects 12-15 years - Systolic BP > 130 mmHg or < 80 mmHg or diastolic BP > 80 or <40

    3. Oxygen saturation of < 93 percent

    • Inability or unwillingness of a participant to give written informed consent.

    Contacts and Locations

    Locations

    Site City State Country Postal Code
    1 University of North Carolina Chapel Hill Chapel Hill North Carolina United States 27514

    Sponsors and Collaborators

    • University of North Carolina, Chapel Hill
    • National Heart, Lung, and Blood Institute (NHLBI)

    Investigators

    • Principal Investigator: Lawrence Ostrowski, PhD, University of North Carolina at Chapel Hil

    Study Documents (Full-Text)

    None provided.

    More Information

    Publications

    None provided.
    Responsible Party:
    University of North Carolina, Chapel Hill
    ClinicalTrials.gov Identifier:
    NCT04901715
    Other Study ID Numbers:
    • 20-3465
    • R01HL117836
    First Posted:
    May 25, 2021
    Last Update Posted:
    Aug 15, 2022
    Last Verified:
    Aug 1, 2022
    Individual Participant Data (IPD) Sharing Statement:
    Yes
    Plan to Share IPD:
    Yes
    Studies a U.S. FDA-regulated Drug Product:
    Yes
    Studies a U.S. FDA-regulated Device Product:
    No
    Product Manufactured in and Exported from the U.S.:
    No
    Keywords provided by University of North Carolina, Chapel Hill
    Additional relevant MeSH terms:

    Study Results

    No Results Posted as of Aug 15, 2022