TAS1R1, TAS1R3, TLR2, TLR4, CA6 Gene Polymorphism on Dental Caries

Sponsor

Recep Tayyip Erdogan University Training and Research Hospital (Other)

Overall Status

Completed

CT.gov ID

NCT04066101

Collaborator

(none)

200

Enrollment

Location

22.1

Actual Duration (Months)

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The aim of this study is to examine the effects of TAS1R1, TAS1R3, TLR2, TLR4, CA6, gene polymorphism on caries risk in adults.

Condition or Disease	Intervention/Treatment	Phase
High Caries Risk Low Caries Risk	Genetic: high caries risk Other: caries activity

Detailed Description

To date, genes can be grouped into categories based on the factor influencing dental caries. The major candidate gene categories to date include enamel formation genes, immune response genes, genes related to saliva, and genes related to taste and dietary habits.

CA6 exists in the human saliva and has a critical role of buffering, immune response and taste.Toll-like receptors are expressed in the tongue gustatory papillae where they may initiate immune responses to pathogens.Genetic variations in the TAS1R1 and TAS1R3 for umami cause perception of these tastes differently.

Study Design

Study Type:

Observational

Actual Enrollment :

200 participants

Observational Model:

Case-Control

Time Perspective:

Cross-Sectional

Official Title:

Variants in Taste Genes on Caries Risk and Caries Activity Status

Actual Study Start Date :

Jan 15, 2018

Actual Primary Completion Date :

Oct 15, 2019

Actual Study Completion Date :

Nov 20, 2019

Arms and Interventions

Arm	Intervention/Treatment
high caries risk 100 adults will be allowed in the high caries risk group according to DMFT index (DMFT ≥ 14)	Genetic: high caries risk DNAs were obtained from epithelial cells in buccal mucosa and were extracted with Genomic DNA Isolation Kit Genotyping for a variant was carried out on a Roche Applied Science LightCycler® 480 (Roche Diagnostics GmbH, Mannheim, Germany) with real-time polymerase reaction of Taqman Other Names: DNA isolation, genotyping Other: caries activity Dental caries lesions of all patient scored as initial, moderate or advanced caries Initial lesion; clinically detectable lesion Moderate; visible signs of enamel breakdown Advanced; enamel was fully cavitaed
low caries risk 100 adults will be allowed in the low caries risk group according to DMFT index (DMFT ≤ 5)	Genetic: high caries risk DNAs were obtained from epithelial cells in buccal mucosa and were extracted with Genomic DNA Isolation Kit Genotyping for a variant was carried out on a Roche Applied Science LightCycler® 480 (Roche Diagnostics GmbH, Mannheim, Germany) with real-time polymerase reaction of Taqman Other Names: DNA isolation, genotyping Other: caries activity Dental caries lesions of all patient scored as initial, moderate or advanced caries Initial lesion; clinically detectable lesion Moderate; visible signs of enamel breakdown Advanced; enamel was fully cavitaed

Arm

Intervention/Treatment

high caries risk

100 adults will be allowed in the high caries risk group according to DMFT index (DMFT ≥ 14)

Genetic: high caries risk

DNAs were obtained from epithelial cells in buccal mucosa and were extracted with Genomic DNA Isolation Kit Genotyping for a variant was carried out on a Roche Applied Science LightCycler® 480 (Roche Diagnostics GmbH, Mannheim, Germany) with real-time polymerase reaction of Taqman

Other Names:

DNA isolation, genotyping

Other: caries activity

Dental caries lesions of all patient scored as initial, moderate or advanced caries Initial lesion; clinically detectable lesion Moderate; visible signs of enamel breakdown Advanced; enamel was fully cavitaed

low caries risk

100 adults will be allowed in the low caries risk group according to DMFT index (DMFT ≤ 5)

Genetic: high caries risk

Other Names:

DNA isolation, genotyping

Other: caries activity

Outcome Measures

Primary Outcome Measures

Past caries experience [4 months]
Each tooth will be examined and recorded as decayed, missing, or filled (DMFT). Caries will be defined in accordance with the World Health Organization (WHO 1997) criteria.

Eligibility Criteria

Criteria

Ages Eligible for Study:

25 Years to 44 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

adult be between 25 and 44 years old
subject has DMFT (Decayed, Missing, Filled, Permanent Tooth) ≥ 5, or, DMFT ≥14
same oral health, diet and socioeconomic status

Exclusion Criteria:

systemic or genetic disorders
orthodontic appliances
pregnant women

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Recep Tayyip Erdogan University	Rize		Turkey	53000

Sponsors and Collaborators

Recep Tayyip Erdogan University Training and Research Hospital

Investigators

Principal Investigator: Gül Yıldız Telatar, Dr., Recep Tayyip Erdogan University Dentistry Faculty

Study Documents (Full-Text)

None provided.

More Information

Additional Information:

Gene-environment Interactions in the Etiology of Dental Caries.

Publications

Yildiz G, Ermis RB, Calapoglu NS, Celik EU, Türel GY. Gene-environment Interactions in the Etiology of Dental Caries. J Dent Res. 2016 Jan;95(1):74-9. doi: 10.1177/0022034515605281. Epub 2015 Sep 16.

Responsible Party:

Gul Yildiz Telatar, Principal Investigator, Recep Tayyip Erdogan University Training and Research Hospital

ClinicalTrials.gov Identifier:

NCT04066101

Other Study ID Numbers:

2016/30

First Posted:

Aug 26, 2019

Last Update Posted:

Apr 15, 2021

Last Verified:

Apr 1, 2021

Individual Participant Data (IPD) Sharing Statement:

Plan to Share IPD:

Studies a U.S. FDA-regulated Drug Product:

Studies a U.S. FDA-regulated Device Product:

Keywords provided by Gul Yildiz Telatar, Principal Investigator, Recep Tayyip Erdogan University Training and Research Hospital

caries risk, gene polymorphisms

Study Results

No Results Posted as of Apr 15, 2021