Tissue Biopsy and Imaging Studies in HIV-Infected Patients

Sponsor
National Institute of Allergy and Infectious Diseases (NIAID) (NIH)
Overall Status
Recruiting
CT.gov ID
NCT00001471
Collaborator
(none)
635
Enrollment
1
Location

Study Details

Study Description

Brief Summary

This study will examine tissue from the tonsils, lymph nodes and large bowel of HIV-infected patients to investigate changes in viral load and certain white blood cells during treatment.

Normal volunteers and HIV-infected patients 18 years of age or older may be eligible for this study. Candidates will be screened with a medical history, physical examination, blood and urine tests and possibly an electrocardiogram (EKG). Blood tests may include HLA typing, a genetic test of immune system markers.

Participants may undergo the following procedures:
  • Blood tests (patients and volunteers)

  • Biopsies The frequency of biopsies for given patients may vary, depending on their specific therapy. Typically, biopsies are done at a single time, or for patients starting a new therapy, biopsies could be performed before starting therapy, during therapy and possibly after completion of therapy.

  • Tonsil biopsies (patients and volunteers) Volunteers will have one tonsil biopsy. Patients will have no more than six tonsil biopsies, with no more than three in a 10-day period. The biopsy is done by an ear, nose and throat specialist as an outpatient procedure. The tonsils are numbed with a local anesthetic, and one to four pieces of tissue are extracted.

  • Lymph node biopsies (patients only) Patients will have no more than four lymph node biopsies, performed no more frequently than once a month. The biopsy is done by a surgeon and may require a 2- to 3-day hospital stay. The skin above the lymph nodes is numbed with a local anesthetic, an incision is made and the tissue is removed. Alternatively, a needle biopsy may be done, in which a small amount of lymph tissue is withdrawn through a special needle injected into the site.

  • Intestinal biopsies (patients and volunteers) Volunteers will have one intestinal biopsy procedure. Patients may have up to six intestinal biopsy procedures, each separated by at least 10 days. This is done by a gastroenterologist as an outpatient procedure. A flexible tube (sigmoidoscope or colonoscope) with a light and special lens at the tip is inserted into the rectum and large bowel. Wire instruments passed through the tube are used to extract small tissue samples.

  • Bronchoalveolar lavage (BAL; patients and volunteers) Volunteers and patients will undergo bronchoscopy in which a flexible tube (bronchoscope) with a light and special lens at the tip is inserted through the nose or mouth into the lungs, and the lining of the lung is sampled by washing the airways with small amounts of saline. The procedure is performed by a pulmonologist or critical care specialist, usually as an outpatient.

Condition or DiseaseIntervention/TreatmentPhase

    Detailed Description

    Examining tissues outside the bloodstream in HIV-infected patients, patients with Idiopathic CD4 lymphopenia (ICL) and, for comparison, HIV-uninfected healthy volunteers (hereafter referred to as healthy volunteers), can provide insights into the pathogenesis of HIV infection and ICL. This protocol will provide a mechanism for sampling tissue sites. A total of 430 HIV-infected patients, 100 ICL patients and 105 healthy volunteers will be enrolled in these studies. To assess changes in viral load and immunological parameters at sites outside the bloodstream during therapy of HIV-infected patients, sequential tonsillar, lymph node, or intestinal biopsies, or bronchoscopy with bronchoalveolar lavage (BAL) will be undertaken during the course of therapy. In a small number of patients, sequential or simultaneous lymph node biopsies will be performed. In addition, uninfected healthy volunteers will be enrolled to have a tonsillar or intestinal biopsy, or bronchoscopy with BAL; this will allow comparison of immunologic parameters in HIVinfected and uninfected tonsillar or intestinal tissues, or BAL fluid. Finally, ICL patients may have tissue sampling to assess lymphocyte distribution and possible function in tissues to better understand the pathogenesis of their lymphopenia. Sequential or simultaneous tissue sampling may occur and longitudinal samples may also be obtained to assess stability in tissue compartments or effect of possible immunomodulatory treatments.

    Study Design

    Study Type:
    Observational
    Anticipated Enrollment :
    635 participants
    Observational Model:
    Cohort
    Time Perspective:
    Prospective
    Official Title:
    Virologic and Immunologic Evaluation of Lymph Node, Tonsillar and Intestinal Biopsies, and Bronchoalveolar Lavage Fluid
    Actual Study Start Date :
    Nov 30, 1994

    Arms and Interventions

    ArmIntervention/Treatment
    Healthy Volunteers

    Healthy Volunteers

    HIV-infected

    HIV-infected individuals

    ICL

    Idiopathic CD4 lymphopenia

    Outcome Measures

    Primary Outcome Measures

    1. Obtain lymphoid tissue by tonsillar, lymph node, or intestinal biopsies, or BAL fluid in patients with HIV, ICL, and healthy volunteers to evaluate for viral burden, lymphocyte subsets, and cytokine production. [Each procedure visit where tissue is obtained]

      Evaluate for viral burden, lymphocyte subsets, and cytokine production.

    Eligibility Criteria

    Criteria

    Ages Eligible for Study:
    18 Years and Older
    Sexes Eligible for Study:
    All
    Accepts Healthy Volunteers:
    Yes
    • INCLUSION CRITERIA:

    Greater than or equal to 18 years old.

    Ability to sign informed consent.

    For women of child-bearing potential, negative result on a serum or urine pregnancy test within 1 week prior to the procedure.

    Willingness to allow storage of blood or biopsy samples for possible future use to study HIV/AIDS, related diseases or the immune system; willingness to permit HLA testing.

    FOR PATIENTS UNDERGOING BIOPSIES:

    No medical contraindication to tonsillar, lymph node, or intestinal biopsy.

    For tonsillar biopsy, presence of visible tonsillar tissue; for lymph node biopsy, palpable lymph nodes.

    No aspirin or piroxicam (Feldene) for 10 days prior to the procedure; other non steroidal anti-inflammatory drugs (e.g. ibuprofen) must be discontinued the day prior to the procedure. Acetaminophen [Tylenol] is permitted at any time.

    FOR PATIENTS UNDERGOING BAL:

    Hematocrit greater than 27 percent, platelets greater than 50,000/ml.

    Baseline pulse-oximetry recording of 94 percent or greater unless clinical indication for bronchoscopy.

    No medical contraindication to bronchoscopy.

    In addition to the above:
    FOR HIV POSITIVE VOLUNTEERS:

    HIV infection must be confirmed by ELISA and western blot or dot blot. For patients with acute HIV infection and negative HIV serology, plasma HIV viral load greater than 10,000 copies/ml.

    FOR HEALTHY VOLUNTEERS:

    No underlying significant medical problem, especially an immunodeficiency or autoimmune disease, or an underlying problem requiring immunosuppressive therapy.

    Absence of HIV infection as confirmed by negative ELISA and, if indicated, western blot or dot blot.

    FOR ICL PATIENTS:

    Patients must meet the definition of ICL according to the CDC criteria: documented absolute CD4 T lymphocyte count of less than 300 cells per cubic millimeter or of less than 20 percent of total T cells on more than one occasion usually two to three months apart, without evidence of HIV infection or any defined immunodeficiency or therapy associated with depressed levels of CD4 T cells.

    Absence of HIV infection as confirmed by negative ELISA and, if indicated, western blot or dot blot.

    EXCLUSION CRITERIA:
    FOR ALL VOLUNTEERS UNDERGOING BIOPSIES:

    Platelet count less than 75,000 platelets/mm(3).

    PT or PTT prolonged by greater than 2 seconds unless patient has documented lupus anticoagulant/anti-phospholipid syndrome, which is not associated with an increased bleeding risk

    Known underlying bleeding disorder.

    Pregnancy.

    FOR HIV-POSITIVE OR ICL VOLUNTEERS FOR LYMPH NODE BIOPSIES:

    Use of narcotics (other than as prescribed by a physician) or cocaine less than 1 week prior to the date of biopsy.

    FOR ALL VOLUNTEERS FOR INTESTINAL BIOPSIES:

    Use of narcotics (other than as prescribed by a physician) or cocaine less than 1 week prior to the date of biopsy.

    Significant heart valve abnormalities.

    Presence of pacemaker, artificial joint or vascular surgery graft.

    FOR ALL VOLUNTEERS FOR BAL:

    Use of narcotics (other than as prescribed by a physician) or cocaine less than 1 week prior to the date of biopsy.

    Pregnancy.

    Any medical condition for which the investigators believe bronchoscopy may be contraindicated.

    Allergy to lidocaine.

    History of asthma requiring therapy.

    Contacts and Locations

    Locations

    SiteCityStateCountryPostal Code
    1National Institutes of Health Clinical Center, 9000 Rockville PikeBethesdaMarylandUnited States20892

    Sponsors and Collaborators

    • National Institute of Allergy and Infectious Diseases (NIAID)

    Investigators

    • Principal Investigator: Joseph A Kovacs, M.D., National Institutes of Health Clinical Center (CC)

    Study Documents (Full-Text)

    None provided.

    More Information

    Additional Information:

    Publications

    Responsible Party:
    National Institute of Allergy and Infectious Diseases (NIAID)
    ClinicalTrials.gov Identifier:
    NCT00001471
    Other Study ID Numbers:
    • 950027
    • 95-I-0027
    First Posted:
    Nov 4, 1999
    Last Update Posted:
    Dec 3, 2021
    Last Verified:
    Jun 30, 2021
    Keywords provided by National Institute of Allergy and Infectious Diseases (NIAID)

    Study Results

    No Results Posted as of Dec 3, 2021