Dysregulated Urea-synthesis at Terminal Uremia
This project will examine the dysregulation of the urea cycle in patients with terminal uremia using a validated method named "Functional Hepatic Nitrogen Clearance"
Arms and Interventions
Alanine infusion taking place over a 3 hour period while monitorering metabolic changes in blood samples before and after dialysis
Other: Aminoacid, alanine-infusion
During 3 hours, patient will receive alanine-infusion, while amino-acid and urea will be monitored by blood samples.
Primary Outcome Measures
- Urea synthesis rate during alanine infusion [4 hours. 1 hour of blood sample measuring of baseline urea-synthesis rate and 3 hours of urea-synthesis rate during alanine-infusion]
Change in the synthesis of urea during a 3 hour period of alanine-infusion in pre and post hemodialysis patients. We will use a validated method named functional hepatic nitrogen clearance (FHNC) to evaluate the synthesis of urea. FHNC is calculated using the linear relation between urea nitrogen synthesis rate and blood-amino acid which is measured every hour. An estimated group of 10 dialysis patients will be included and compared to a clinical healthy group. The healthy group has also received alanine infusion and blood samples of urea and amino-acids has likewise been measured for every hour of infusion.
Age > or equal to 18 years
Chronic hemodialysis during 3 months
Patients with functioning arteriovenous-fistula assigned to "Center-Hemodialysis" at "Nyresygdomme, AUH Skejby"
Conditions with vomiting or diarrhea
Active disease being treated with chemotherapy, radiationtherapy, biologic- or similar treatments. Disease under observation can be included.
Vessels on the upper extremities not suited for cannulation
Prednisolone treatment during the last 8 weeks
BMI > or equal to 30 or < or equal to 18
Physically or mentally condition that does not allow the project to be completed
Language-difficulties that does not allow the project to be completed
Contacts and Locations
|1||University Hospital Aarhus||Aarhus N||Denmark||8200|
Sponsors and Collaborators
- University of Aarhus
Study Documents (Full-Text)None provided.
- Dysregulated urea-synthesis