Utilizing Free DNA in Embryo Culture for PGT
Study Details
Study Description
Brief Summary
In the way for developing and optimizing protocol to be used as non- invasive methodology used as routine testing for PGS.
This protocol is to be adapted to replace the using of life embryo cells for genetic testing and aneuploidy study as well as for any type of genetic testing including single gene disorder or HLA typing or study.
Condition or Disease | Intervention/Treatment | Phase |
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Detailed Description
Compare 100 embryos studied using NGS for chromosomal aneuploidies, and compare the results came from free media DNA with this results.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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NGS reading for Media Free DNA Ability to read the DNA product available in embryo culture media using NGS to have valuable diagnostic image for genetic composition of embryo before implantation. |
Procedure: NGS reading for Media Free DNA
NGS reading for Media Free DNA instead of blstomer biopsy
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NGS reading for blastomer DNA DNA extracted from Blastomere used for aneuploidy screening. |
Procedure: NGS reading for Media Free DNA
NGS reading for Media Free DNA instead of blstomer biopsy
|
Outcome Measures
Primary Outcome Measures
- DNA isolation [2 years]
reading amplified DNA using NGS
Secondary Outcome Measures
- NGS evaluation [2 years]
Free DNA isolation and amplification using WGA
- Comparison with cell reading using NGS [2 years]
compare the obtained result with the results provided collected for the same embryo using its blastomer for PGS
Eligibility Criteria
Criteria
Inclusion Criteria:
- all abnormal/aneuploidy embryos rejected for embryo transfer.
Exclusion Criteria:
- all embryos had unsuccessful diagnosis with blastomere biopsy.
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- Al Baraka Fertility Hospital
- Ibn Sina Hospital
- Sadat City University
Investigators
- Study Director: Omima Khamis, PHD, Genetic Engineering Institute
Study Documents (Full-Text)
None provided.More Information
Additional Information:
Publications
None provided.- NGS-PGT