DOTTI: Administration of Donor T Cells With the Caspase-9 Suicide Gene

Sponsor

Baylor College of Medicine (Other)

Overall Status

Active, not recruiting

CT.gov ID

NCT01494103

Collaborator

The Methodist Hospital Research Institute (Other), Center for Cell and Gene Therapy, Baylor College of Medicine (Other)

Enrollment

Locations

Arm

210

Duration (Months)

7.5

Patients Per Site

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Patients will be receiving a stem cell transplant as treatment for their disease. As part of the stem cell transplant, patients will be given very strong doses of chemotherapy, which will kill all their existing stem cells.

A close relative of the patient will be identified, whose stem cells are not a perfect match for the patient's, but can be used. This type of transplant is called "allogeneic", meaning that the cells are from a donor. With this type of donor who is not a perfect match, there is typically an increased risk of developing GvHD, and a longer delay in the recovery of the immune system.

GvHD is a serious and sometimes fatal side-effect of stem cell transplant. GvHD occurs when the new donor cells (graft) recognize that the body tissues of the patient (host) are different from those of the donor.

In this study, investigators are trying to see whether they can make special T cells in the laboratory that can be given to the patient to help their immune system recover faster. As a safety measure, we want to "program" the T cells so that if, after they have been given to the patient, they start to cause GvHD, we can destroy them ("suicide gene").

Investigators will obtain T cells from a donor, culture them in the laboratory, and then introduce the "suicide gene" which makes the cells sensitive to a specific drug called AP1903. If the specially modified T cells begin to cause GvHD, the investigators can kill the cells by administering AP1903 to the patient. We have had encouraging results in a previous study regarding the effective elimination of T cells causing GvHD, while sparing a sufficient number of T cells to fight infection and potentially cancer.

More specifically, T cells made to carry a gene called iCasp9 can be killed when they encounter the drug AP1903. To get the iCasp9 gene into T cells, we insert it using a virus called a retrovirus that has been made for this study. The AP1903 that will be used to "activate" the iCasp9 is an experimental drug that has been tested in a study in normal donors with no bad side-effects. We hope we can use this drug to kill the T cells.

The major purpose of this study is to find a safe and effective dose of "iCasp9" T cells that can be given to patients who receive an allogeneic stem cell transplant. Another important purpose of this study is to find out whether these special T cells can help the patient's immune system recover faster after the transplant than they would have otherwise.

Condition or Disease	Intervention/Treatment	Phase
Acute Lymphoblastic Leukemia Myelodysplastic Syndrome Acute Myeloid Leukemia Chronic Myelogenous Leukemia Non Hodgkin Lymphoma Hemophagocytic Lymphohistiocytosis Familial Hemophagocytic Lymphohistiocytosis Hemophagocytic Syndrome Epstein Barr Virus Infection X-linked Lymphoproliferative Disease	Biological: iCaspase9-transduced T cells Drug: AP1903	Phase 1

Detailed Description

If the patient is doing well after the stem cell transplant, and does not have severe GvHD, s/he will be eligible to receive the special "iCasp9" T cells from Day 30 to 90 after transplant. The specially selected and treated T cells will be given by vein (IV) once.

This is a dose escalation study. This means that at the beginning, patients will be started on the lowest dose (1 of 5 different levels) of T cells. Once that dose schedule proves safe, the next group of patients will be started at a higher dose. This process will continue until all 5 dose levels are studied. If the side-effects are too severe, the dose will be lowered or the T cell injections will be stopped.

If the patient develops GvHD after being given the specially treated T cells, we will prescribe AP1903, which has been shown to kill cells carrying the iCasp9 gene. This drug will be given as a 2-hour IV infusion.

We will continue to follow the patient weekly in the bone marrow transplant clinic for the first month after the infusion, to check for side-effects of the treatment and for GvHD. The patient will have the standard tests performed that all patients have after transplant, even when not receiving special T cells.

Study Design

Study Type:

Interventional

Actual Enrollment :

15 participants

Allocation:

N/A

Intervention Model:

Single Group Assignment

Masking:

None (Open Label)

Primary Purpose:

Treatment

Official Title:

Administration of Haploidentical Donor T Cells Transduced With the Inducible Caspase-9 Suicide Gene

Study Start Date :

Nov 1, 2011

Anticipated Primary Completion Date :

May 1, 2029

Anticipated Study Completion Date :

May 1, 2029

Arms and Interventions

Arm	Intervention/Treatment
Experimental: iCaspase9-transduced T cells The 5 dose levels are: 1 x 10^4 T cells/kg 1 x 10^5 T cells/kg 5 x 10^5 T cells/kg 1 x 10^6 T cells/kg 5 x 10^6 T cells/kg AP1903 will be administered if there is development of Grade 1 or greater GvHD.	Biological: iCaspase9-transduced T cells Patients will receive the T cells between 30 and 90 days following transplantation. The T cells will be infused through a catheter line. Drug: AP1903 AP1903 will be administered if there is development of Grade 1 or greater GvHD. Dose: 0.4 mg/kg by IV over 2 hours. Up to 3 additional doses may be administered if the GvHD does not respond or gets worse. Other Names: AP1903 Dimerizer Drug

Arm

Intervention/Treatment

Experimental: iCaspase9-transduced T cells

The 5 dose levels are: 1 x 10^4 T cells/kg 1 x 10^5 T cells/kg 5 x 10^5 T cells/kg 1 x 10^6 T cells/kg 5 x 10^6 T cells/kg AP1903 will be administered if there is development of Grade 1 or greater GvHD.

Biological: iCaspase9-transduced T cells

Patients will receive the T cells between 30 and 90 days following transplantation. The T cells will be infused through a catheter line.

Drug: AP1903

AP1903 will be administered if there is development of Grade 1 or greater GvHD. Dose: 0.4 mg/kg by IV over 2 hours. Up to 3 additional doses may be administered if the GvHD does not respond or gets worse.

Other Names:

AP1903 Dimerizer Drug

Outcome Measures

Primary Outcome Measures

Clinical and immunological effects of AP1903 administration. [14 days]
To evaluate the clinical and immunological effects of AP1903 administration, a dimerizer drug used to activate an iCaspase9 suicide gene mechanism, to subjects who have received escalating doses of T lymphocytes expressing the iCaspase9 gene and developed acute graft-versus-host-disease (GvHD).

Secondary Outcome Measures

T cell dose that produces a greater than 25% risk of Grade II or greater GvHD. [42 days]
To discover the number of T cells/kg (up to 5 x 10^6/kg) that produce a greater than 25% risk of inducing Grade II or greater acute GvHD in these subjects.
Immune reconstitution and relative contribution of iCaspase9-modified T cells post-infusion. [Up to 15 years]
To measure the subsequent immune reconstitution of recipients of iCaspase9 modified T cells (and dimerizer drug), and assess the relative contribution of endogenous T cell recovery and infused gene-modified T cells.
Overall and disease-free survival. [100 days and 1 year]
To measure the overall and disease-free survival of recipients of iCaspase9 T cells at 100 days and at 1 year post-transplant.

Eligibility Criteria

Criteria

Ages Eligible for Study:

N/A and Older

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria:

Inclusion Criteria at Time of Procurement:

Lack of a suitable conventional donor (i.e. 5/6 or 6/6 related, or 5/6 or 6/6 unrelated donor), or presence of a rapidly progressive disease not permitting time to identify an unrelated donor.
High risk disease in one of the following:

Myelodysplastic syndrome (MDS) in one of the following categories: RCMD with an IPSS-R of intermediate, poor, or very poor, RAEB-1, or RAEB-2
Acute myeloid leukemia (AML) after first relapse or primary refractory disease
Chronic myelogenous leukemia (CML) in Chronic Phase 2 or greater, Accelerated Phase or Blast Crisis
Acute lymphoblastic leukemia (ALL) after first relapse or primary refractory disease, or High-Grade Non Hodgkin lymphoma (NHL) Stage III or IV after first relapse or primary refractory disease
Hemophagocytic lymphohistiocytosis (HLH)
Familial hemophagocytic lymphohistiocytosis (FLH)
Viral-associated hemophagocytic syndrome (VAHS)
T or NK cell lymphoproliferative syndrome
X-linked lymphoproliferative disease (XLP)

Inclusion Criteria at Time of T Cell Infusion:

Engrafted with an absolute neutrophil count (ANC) > 500 cells/µL
Greater than or equal to 50% donor chimerism in either peripheral blood or bone marrow, or relapse of their original disease
Life expectancy > 30 days
Lansky/Karnofsky score greater than or equal to 60
Absence of severe renal disease (creatinine > 2X upper limit of normal for age)
Absence of severe hepatic disease (direct bilirubin > 3X upper limit of normal or SGOT

3X upper limit of normal)

Oxygen saturation > 94% on room air
Patient/Guardian able to give informed consent
AP1903 available in sufficient quantities to allow for treatment of the patient

Exclusion Criteria:

Exclusion Criteria at Time of T Cell Infusion:

GvHD
Severe intercurrent infection
Pregnancy*
Other investigational drugs in the prior 30 days

Pregnancy test only required for at-risk individuals, defined as female patients of childbearing potential who have received a reduced-intensity conditioning regimen.