COLDBAT: Cold Induced Activation of Brown Adipose Tissue in Humans
Study Details
Study Description
Brief Summary
This study investigates cold-induced brown fat activation assessed using PET/MR scans. Subjects will participate in an acute cooling intervention day and a thermoneutral intervention day with PET/MR scans on both days. A secondary purpose is to make a validation of an infrared thermography camera by comparison of skin temperatures and SUV of the supraclavicular brown adipose tissue.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
The field of human brown adipose tissue (BAT) research is focused on activation of BAT as a means of manipulating energy expenditure and potentially anti-obesity and anti-diabetic properties of the tissue. This is well established in rodent studies and explained as due to the specific brown fat uncoupling protein 1 (UCP1). Despite increasing evidence that indicates a metabolic regulatory role of BAT in humans, BAT activation/recruitment is not fully understood and even less is known about the endocrine capacities of human BAT.
Cold induced brown fat activity will be assessed with PET/MR scans and validate an infra-red thermography camera, as well as perform a proteomic screening using blood samples under these two intervention days.
15 healthy lean male subjects will serve as their own control in a cross-over randomized control study, including a pre-examination day, a thermoneutral-day and a cooling test-day with cold exposure followed by a PET/MR scan to determine BAT activity. An extra cooling day is applied for the external validation of the infrared thermography camera. Blood samples and abdominal fat-biopsies will be taken at thermoneutral condition and under cold stimulation of brown fat.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: Cooling Individualized cooling protocol. |
Procedure: Cooling
Individualized cooling protocol.
Other Names:
|
| Experimental: Thermoneutral Thermoneutral condition day. |
Procedure: Thermoneutral
Thermoneutral control day.
|
Outcome Measures
Primary Outcome Measures
- Difference in brown adipose tissue (BAT) activity after cooling versus no cooling. [Difference in SUV mean after 2 hours of cooling versus 2 hours of no cooling.]
BAT activity is measured by standard fluorodeoxyglukose uptake volume (SUV mean) in the supraclavicular BAT depot using PET/MR scans. Difference between scans performed after 2 hours of cooling and 2 hours of no cooling is calculated.
Secondary Outcome Measures
- Difference in BAT activity after cooling versus no cooling. [Difference in peak temperature after 2 hours of cooling versus 2 hours of no cooling.]
BAT activity is measured by skin temperature in the supraclavicular BAT depot using infrared thermography imaging. Difference between peak temperature change (peak temperature minus baseline temperature) during 2 hours of cooling and 2 hours of no cooling is calculated.
- Difference in resting energy expenditure after cooling versus no cooling [Difference in resting energy expenditure after 2 hours of cooling versus 2 hours of no cooling.]
Difference in resting energy expenditure after cooling versus no cooling, measured as mean difference in KJ/min.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Age 18-30 years.
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Men
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Caucasian white
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Body Mass Index 19-25 kg/m2.
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Moderate physical activity level (not more than 8-10 hours of training pr. Week)
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Ability to give informed consent.
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Ability to follow verbal and written instructions in Danish.
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Body fat percent under 21% measured at the pre-examination day.
Exclusion Criteria:
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Chronic diseases: Hormonal: Metabolic Diseases mb. Graves, hypothyroidism, myxedema and goiter. Diabetes Melitus, Coronary heart disease (history of myocardial infarction, unstable angina pectoris, or congestive heart failure), cancer treated within last 3 years.
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Tobacco, marijuana or intravenous drug use within 1 year of screening.
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Dieting, recent weight loss (>3 kg within 3 month) or a history of an eating disorder.
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Dietary supplements.
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History of depression, psychosis, or other psychiatric illness requiring hospitalization.
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Alcohol consumption >14 units/week.
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History of alcohol abuse within the past 3 years.
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Known liver disease or elevated liver biomarkers more than 2 times upper normal levels in the preliminary investigation.
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Known kidney disease or elevated kidney biomarkers, eCRF <60 ml/min, in the preliminary investigation.
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Use of daily medications, except for seasonal use of antihistamines.
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Diagnosed sleep disturbances.
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Pacemaker or other electronics implanted in the body.
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Magnetic impressionable metal implants or foreign objects in the body inconsistent with completion of MR.
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Claustrophobia
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Communication problems, including understanding the experimental protocol
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History of contrast allergy.
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Winter swimmers.
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Center for Inflammation and Metabolism/ Center for Physical Activity Research | Copenhagen | Denmark | 2100 |
Sponsors and Collaborators
- Susanna Søberg
Investigators
- Principal Investigator: Susanna Søberg, MSc., Center for Inflammation and Metabolism/Center for physical activity research
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- H-16038581-COLDBAT