EAT: Expandability of Aging Adipose Tissue
Study Details
Study Description
Brief Summary
The purpose of this pilot study is to determine whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. The investigators hypothesize that the adipose tissue response to overfeeding may be weakened in older when compared to younger men, thereby leading to worsened ectopic storage depots for older men.
Detailed Description
This pilot study will begin to address whether adipose tissue biology changes with advancing age. Specifically, we will test whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. To test this possibility the Investigators will recruit overweight and mildly obese older (age 55-75y) and younger (age 25-40y) men and collect adipose tissue biopsies before and after 7 days of overfeeding. The investigators will test how well the cells in adipose tissue expand in response to overfeeding and whether this differs between younger and older men.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Other: MA Middle-aged overweight and obese men (age 25-40yr) |
Other: Overfeeding
Consumption of 40% excess calories for 7 consecutive days
|
| Other: OA Older overweight and obese men (age 55-75yr) |
Other: Overfeeding
Consumption of 40% excess calories for 7 consecutive days
|
Outcome Measures
Primary Outcome Measures
- Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+) [1 wk of overfeeding]
age-related difference in the ASC differentiation response to overfeeding
Secondary Outcome Measures
- Change in ASC proliferation (Delta rate of cell growth) [1 wk of overfeeding]
age-related difference in the ASC proliferative response to overfeeding
- Change in expression of C/EBPalpha, C/EBPbeta, PPARgamma2, SREBP1c, SREBf1a, and Wnt10b (fold-changes in mRNA expression) [1 wk overfeeding]
age-related difference in the pro-adipogenic response to overfeeding
- Change in intramyocellular (IMCL) di-saturated diacylglycerol (DAG) localization (Delta %membrane:cytosol) [1 week overfeeding]
age-related difference in the IMCL DAG response to overfeeding
Eligibility Criteria
Criteria
Inclusion Criteria:
-
Men
-
Aged 25-40y OR 55-75y
-
BMI 28-35 kg/m2
-
Weight stable (±2kg over past 3 mo)
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Sedentary to moderately active (<3 d/wk of exercise lasting >20min)
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Non-smokers (never used or quit >7yrs prior)
Exclusion Criteria:
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Women
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Currently using hormones, glucose or lipid lowering medication
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Engaged in exercise or diet program
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Currently gaining or losing weight or using weight loss drugs
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Diabetes mellitus (diagnosis, treatment, FBG ≥126 mg/dL)
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Uncontrolled metabolic disorders (e.g., thyroid)
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Univeristy of Colorado, Anschutz Medical Campus | Aurora | Colorado | United States | 80045 |
Sponsors and Collaborators
- University of Colorado, Denver
- Colorado State University
Investigators
- Principal Investigator: Rachael E Van Pelt, PhD, University of Colorado, Denver
Study Documents (Full-Text)
None provided.More Information
Publications
- Caso G, McNurlan MA, Mileva I, Zemlyak A, Mynarcik DC, Gelato MC. Peripheral fat loss and decline in adipogenesis in older humans. Metabolism. 2013 Mar;62(3):337-40. doi: 10.1016/j.metabol.2012.08.007. Epub 2012 Sep 19.
- Kim JY, van de Wall E, Laplante M, Azzara A, Trujillo ME, Hofmann SM, Schraw T, Durand JL, Li H, Li G, Jelicks LA, Mehler MF, Hui DY, Deshaies Y, Shulman GI, Schwartz GJ, Scherer PE. Obesity-associated improvements in metabolic profile through expansion of adipose tissue. J Clin Invest. 2007 Sep;117(9):2621-37.
- Tchoukalova YD, Votruba SB, Tchkonia T, Giorgadze N, Kirkland JL, Jensen MD. Regional differences in cellular mechanisms of adipose tissue gain with overfeeding. Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):18226-31. doi: 10.1073/pnas.1005259107. Epub 2010 Oct 4.
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