Safety Study of Depigoid Vaccine Dermatophagoides Pteronyssinus or 50% Dermatophagoides Pteronyssinus / 50% Dermatophagoides Farinae (500 DPP/ml), to Treat Allergic Rhinitis or Rhinoconjunctivitis With or Without Asthma

Sponsor

Laboratorios Leti, S.L. (Industry)

Overall Status

Terminated

CT.gov ID

NCT01591343

Collaborator

Harrison Clinical Research (Industry)

Enrollment

Locations

Arm

Duration (Days)

0.8

Patients Per Site

0.8

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Safety study of Depigoid vaccine Dermatophagoides pteronyssinus or 50% Dermatophagoides pteronyssinus / 50% Dermatophagoides farinae (500 DPP/ml), to treat allergic rhinitis or rhinoconjunctivitis with or without asthma.

Primary variable: number of subjects [%] who experienced at least one immediate or delayed systemic reaction of EAACI grade 2 or higher during the 4-month treatment period.

Condition or Disease	Intervention/Treatment	Phase
Allergic Rhinitis Rhinoconjunctivitis Mild Persistent Asthma Mild Intermittent Asthma	Biological: Depigoid® (500 DPP/ml)	Phase 2

Detailed Description

Investigator(s):

Approximately 10 study centers (allergy services) in Spain

Planned study period:

MAY 2012 (first subject in) - MAR 2013 (last subject out)

Objectives:

Primary objective: to evaluate the safety of a 4-month treatment with an extract of Depigoid® Dermatophagoides pteronyssinus or a mixture of 50% Dermatophagoides pteronyssinus and 50% Dermatophagoides farinae at a concentration of 500 DPP/ml administered following a rush build-up regimen.

Secondary objective: to assess the subjects' immunologic responses to the above treatment.

Study design:

This is a prospective, non-randomized, uncontrolled, open-label safety study. When the first 29 subjects have completed the study, an interim analysis will be conducted to check whether the number of systemic reactions in these subjects is below a predefined limit.

Planned number of subjects:

103 subjects in total.

Medical condition or disease under investigation:

Allergic rhinitis or rhinoconjunctivitis, with or without mild persistent or intermittent asthma.

Test product, dose and mode of administration:

Depigoid® D. pteronyssinus or Depigoid® 50% D. pteronyssinus / 50% D. farinae (500 DPP/ml).

Dose: Week 0: 0.2 ml followed by 0.3 ml after 30 min Week 4, 8, 12, and 16: 0.5 ml Mode of administration: subcutaneous injection

Duration of treatment:

16 weeks.

Criteria for evaluation:

Safety:

Local and systemic adverse reactions (EAACI classification); adverse events.

Efficacy:

Immunologic response to the treatment.

Statistical methods:

Primary variable: number of subjects [%] who experienced at least one immediate or delayed systemic reaction of EAACI grade 2 or higher during the 4-month treatment period.

Analysis of primary variable: exact binomial test

Study Design

Study Type:

Interventional

Actual Enrollment :

8 participants

Allocation:

N/A

Intervention Model:

Single Group Assignment

Masking:

None (Open Label)

Primary Purpose:

Prevention

Official Title:

Prospective Study to Evaluate the Safety of a 4-month Treatment With Depigoid® Dermatophagoides Pteronyssinus or 50% Dermatophagoides Pteronyssinus / 50% Dermatophagoides Farinae (500 DPP/ml) in Patients With Allergic Rhinitis or Rhinoconjunctivitis With or Without Mild Persistent or Intermittent Asthma

Study Start Date :

Jun 1, 2012

Actual Primary Completion Date :

Jul 1, 2012

Actual Study Completion Date :

Jul 1, 2012

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Depigoid® (500 DPP/ml) Patients will receive either Depigoid® Dermatophagoides pteronyssinus or 50% Dermatophagoides pteronyssinus / 50% Dermatophagoides farinae (500 DPP/ml), depending on their sensitization to one or both mites (Dermatophagoides pteronyssinus and Dermatophagoides farinae).	Biological: Depigoid® (500 DPP/ml) Depigoid® D. pteronyssinus or Depigoid® 50% D. pteronyssinus / 50% D. farinae (500 DPP/ml). Dose: Week 0: 0.2 ml followed by 0.3 ml after 30 min Week 4, 8, 12, and 16: 0.5 ml Mode of administration: subcutaneous injection

Arm

Intervention/Treatment

Experimental: Depigoid® (500 DPP/ml)

Patients will receive either Depigoid® Dermatophagoides pteronyssinus or 50% Dermatophagoides pteronyssinus / 50% Dermatophagoides farinae (500 DPP/ml), depending on their sensitization to one or both mites (Dermatophagoides pteronyssinus and Dermatophagoides farinae).

Biological: Depigoid® (500 DPP/ml)

Depigoid® D. pteronyssinus or Depigoid® 50% D. pteronyssinus / 50% D. farinae (500 DPP/ml). Dose: Week 0: 0.2 ml followed by 0.3 ml after 30 min Week 4, 8, 12, and 16: 0.5 ml Mode of administration: subcutaneous injection

Outcome Measures

Primary Outcome Measures

Primary variable [4 months]
Number of subjects [%] who experienced at least one immediate or delayed systemic reaction of EAACI grade 2 or higher during the 4-month treatment period.

Secondary Outcome Measures

Secondary variables [4 months]
Secondary variables, which will be analyzed descriptively, are the Number of subjects [%] suffering immediate and/or delayed systemic reactions broken down by grade (EAACI classification). Number of subjects [%] suffering immediate and/or delayed local reactions broken down by diameter (< 5 cm, 5-10 cm or > 10 cm). Number of immediate and/or delayed systemic reactions broken down by grade (EAACI classification). Number of immediate and/or or delayed local reactions broken down by diameter (< 5 cm, 5-10 cm and > 10 cm). Immunologic response to the treatment.

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 55 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria:

Men and women between 18 and 55 years of age (both inclusive).
Individuals suffering symptoms of allergic rhinoconjunctivitis or rhinitis during at least the preceding year -- with or without symptoms of mild persistent or intermittent allergic asthma which is controlled with a dose less or equal to 400 µg/day budesonide or an equivalent -- caused by a clinically relevant sensitization to house dust mites (Dermatophagoides pteronyssinus or Dermatophagoides pteronyssinus and Dermatophagoides farinae).
The IgE-mediated sensitization will be demonstrated by means of the following: medical history and IgE specific to house dust mites (D. pteronyssinus or D. pteronyssinus and

farinae) CAP RAST ≥ 2 and positive skin prick test. A skin prick test will be considered positive when it produces a wheal of at least 3 mm according to the largest diameter.

Asthmatic patients must be stable and on a stable inhaled steroid dose within 6 weeks prior to visit 1 and throughout the study.

Exclusion Criteria:

Any contraindication for treatment with allergen specific immunotherapy.
Forced expiratory volume in 1 s (FEV1) or peak expiratory flow (PEF) value < 80% of the predicted normal value.
Allergy symptoms due to sensitization to pollens or other perennial allergens (molds, epithelia).
Subjects with typical symptoms to co-allergens such as tree pollen, grasses or weeds, fungi or animal epithelial cells cannot participate in this study. Patients sensitized to pollens with specific IgE CAP RAST < 2 can be included in this study provided that they do not present symptoms as a consequence of this sensitization. Patients sensitized to animal epithelia who do not present symptoms can participate in this study provided that they are not exposed to the allergen to which they are sensitized, even though they present specific IgE with CAP RAST ≥ 2. If they were exposed to the animal in question, these subjects must have a specific IgE with CAP RAST < 2 in order to participate in the study.
Asthma requiring a dose > 400 µg/day of Budesonide or an equivalent, without long-lasting beta-2 agonists, to reach control according to the Global Initiative for Asthma (GINA 2010).
Patients with non controlled bronchial asthma within 3 months prior to Visit 1.
Patients with asthma who have been treated with systemic steroids within 3 months prior to V1.
Patients with hospital admission due to asthma exacerbations within 1 year prior to V1
Acute or chronic inflammatory or infectious diseases of the airways.
Chronic structural diseases of the respiratory system (for example, emphysema or bronchiectasis).
Immune system diseases, both autoimmune diseases and immunodeficiency.
Any disease involving a contraindication for the use of adrenaline (for example, hyperthyroidism).
Serious uncontrolled diseases involving a risk for the subjects participating in this study, including the following for example: heart failure, serious or uncontrolled respiratory diseases, endocrine diseases, clinically relevant liver or kidney diseases or hematological diseases.
Malignant disease with activity in the last 5 years.
Clinically significant anomaly in laboratory parameters or vital signs which could involve a risk increase for the subject.
Excessive consumption of alcohol, drugs or medication in the preceding year.
Serious psychiatric, psychological or neurological disorders.
Use of immunotherapy with allergenic extracts of storage or house dust mites in the last 5 years.
Systemic or topical treatment with beta-blocker drugs.
Treatment with substances interfering with the immune system 2 weeks before visit 2.
Use of psychotropic or antidepressants substances.
Use of systemic corticosteroids 3 months before visit 1.
Immunization with prophylactic (bacterial or viral) vaccines within 7 days before visit 2. (Prophylactic vaccines are allowed during the administration of IMP period provided they are administered at least one week after immunotherapy and the next immunotherapy dose is administered at least 14 days later).
Participation of the subject in another clinical trial 30 days before visit 2.
Subjects who have already participated in this clinical trial.
Subjects who are going to donate stem cells, blood, organs or bone marrow in the course of the study.
Female subjects who are pregnant or nursing and women with a positive pregnancy test at visit 1 or 2.
Women of childbearing potential not using highly effective methods of birth control. Highly effective methods of birth control are defined as those which result in a low failure rate (i.e. less than 1% per year) when used consistently and correctly, such as implants, injectables, combined oral contraceptives, some IUDs, sexual abstinence or vasectomized partner. Female patients will be considered to be of childbearing potential unless surgically sterilized by hysterectomy or bilateral tubal ligation, or post-menopausal for at least two years.
No written informed consent upon enrolment.
Not willing to give consent for transmission of personal "pseudonymised" data.
Subjects who are unable to comply with the requirements of the study or who in the opinion of the investigator should not participate in the study.

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Complejo Hospitalario Torrecárdenas	Almería	Andalucía	Spain	04009
2	Clínica Doctor Lobatón	Cádiz	Andalucía	Spain	11008
3	Hospita Universitario Dr. Negrín	Las Palmas de Gran Canaria	Canarias	Spain	35010
4	Hospital Universitario Marqués de Valdecilla	Santander	Cantabria	Spain	39008
5	Al·lergo Centre	Barcelona	Cataluña	Spain	08021
6	Centro Médico Teknon	Barcelona	Cataluña	Spain	08022
7	Hospital Universitario Vall d'Hebrón	Barcelona	Cataluña	Spain	08035
8	Hospital Universitario de Bellvitge	Barcelona	Cataluña	Spain	08907
9	Hospital Universitario Joan XXIII	Tarragona	Cataluña	Spain	43007
10	Complejo Hospitalario Universitario de Cartagena	Cartagena	Murcia	Spain	03020