Association of Intestinal Microbiota and the Onset of Perianal Abscess Based on 16S rDNA Amplicon Sequencing
Study Details
Study Description
Brief Summary
The goal of this observational study is tolearn the association of gut microflora with the onset of perianal abscesses. The main questions it aims to answer are:
Question 1: To understand the structure of rectal microbial community composition and its relationship with pathogenic bacteria in patients with perianal abscess.
Question 2: Understand the structure of rectal microbial community composition in healthy people.
Participants will be collected with rectal secretions and stool specimens.In addition, patients with perianal abscess should also collect pus samples.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| observation group This group will collect rectal secretions and stool samples and pus samples from patients with perianal abscess before treatment, and collect rectal secretions and stool samples from patients again 2 months after treatment according to the diagnosis and treatment guidelines. |
Procedure: surgical operation
Radical operation for the perianal abscess
|
| control group This group collected rectal secretions and stool specimens from a healthy population. |
Outcome Measures
Primary Outcome Measures
- Observation group: the structure and changes of rectal flora before and after treatment [Two months]
Analysis of the intestinal microflora based on 16S rDNA amplicon sequencing
- Control group: the structure and changes of rectal flora in healthy population [Two months]
Analysis of the intestinal microflora based on 16S rDNA amplicon sequencing
Eligibility Criteria
Criteria
Inclusion Criteria:
- Observation group: ① Age between 18-60 years, local resident of Xi'an; ② met the diagnostic criteria for perianal abscess and underwent surgical treatment in our hospital; ③ did not use antibiotics before treatment; ④ himself and his family members were willing to participate in this study and signed informed consent.
Control group: ① Age between 18-60 years, permanent residents in Xi'an; ② had no serious chronic disease, no abnormality in physical examination in the last 1 week; ③ did not use drugs that may affect intestinal flora in the last one month; ④ himself and his family members are willing to participate in the study and sign the informed consent.
Exclusion Criteria:
- Failing to retain the specimens as required
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- Xi'an Hospital of Traditional Chinese Medicine
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 2022SF-369