Autophagy Markers in Endometrial Polyps
Study Details
Study Description
Brief Summary
The aim of this research is to evaluate autophagy markers in patients with endometrial polyps
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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Detailed Description
Endometrial polyp are benign formations that are frequently encountered in gynecology practice and have malignant potential. It may be asymptomatic in the clinic or may present with abnormal uterine bleeding. Although its pathophysiology has not been clearly revealed, there are many studies in the literature about it. However, the autophagy pathway emerges as a subject that has never been evaluated. In this study, the investigators would like to prospectively evaluate Beclin 1, LC3A / B and p62 markers in the autophagy pathway in endometrial polyp tissue samples by immunohistochemistry and reveal their importance.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Study group endometrial polyp |
Genetic: Beclin 1 gene
The determination of Beclin 1, LC3-II and p62 genes, which are evaluated by immunohistochemistry, at the protein level will be done by ELISA method in MSKU Medical Faculty Medical Biology Laboratory.
Other Names:
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| Control group normal endometrium |
Genetic: Beclin 1 gene
The determination of Beclin 1, LC3-II and p62 genes, which are evaluated by immunohistochemistry, at the protein level will be done by ELISA method in MSKU Medical Faculty Medical Biology Laboratory.
Other Names:
|
Outcome Measures
Primary Outcome Measures
- autophagy marker [through study completion, an average of 1 year]
Beclin 1 gene level
- autophagy marker [through study completion, an average of 1 year]
LC3II gene level
- autophagy marker [through study completion, an average of 1 year]
p62 gene level
Eligibility Criteria
Criteria
Inclusion Criteria:
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Patients over 35 years of age with a histopathologically confirmed diagnosis of endometrial polyp
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patients over the age of 35 whose histopathologically reported normal endometrial biopsy results.
Exclusion Criteria:
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Are under the age of 35,
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those with cognitive dysfunction,
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diagnosed with any type of cancer
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who are under treatment for cancer,
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diabetes mellitus,
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hypertension,
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endometriosis,
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adenomyosis,
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chronic endometritis,
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have an acute or chronic illness,
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patients who have smoked and used alcohol in the last 10 years
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Mugla Sıtkı Kocman University Faculty of Medicine | Muğla | Turkey | 48000 |
Sponsors and Collaborators
- Muğla Sıtkı Koçman University
Investigators
- Principal Investigator: Burak SEZGİN, MD, Muğla Sıtkı Koçman University
Study Documents (Full-Text)
None provided.More Information
Publications
- Wong M, Crnobrnja B, Liberale V, Dharmarajah K, Widschwendter M, Jurkovic D. The natural history of endometrial polyps. Hum Reprod. 2017 Feb;32(2):340-345. doi: 10.1093/humrep/dew307. Epub 2016 Dec 18.
- Xie Z, Klionsky DJ. Autophagosome formation: core machinery and adaptations. Nat Cell Biol. 2007 Oct;9(10):1102-9. Review.
- Zhan L, Yao S, Sun S, Su Q, Li J, Wei B. NLRC5 and autophagy combined as possible predictors in patients with endometriosis. Fertil Steril. 2018 Oct;110(5):949-956. doi: 10.1016/j.fertnstert.2018.06.028.
- Zhang J. Teaching the basics of autophagy and mitophagy to redox biologists--mechanisms and experimental approaches. Redox Biol. 2015;4:242-59. doi: 10.1016/j.redox.2015.01.003. Epub 2015 Jan 13. Review.
- 03.09.20-08/01