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Biofilm Formation and Antibiotic Sensitivity Patterns

Sponsor
Sohag University (Other)
Overall Status
Not yet recruiting
CT.gov ID
NCT05181163
Collaborator
(none)
100
Enrollment
12
Anticipated Duration (Months)

Study Details

Study Description

Brief Summary

Detection of bacteria that form biofilm and its sensitivity to antibiotics in patients with surgical site infection after orthopedic surgery.

Condition or Disease Intervention/Treatment Phase
  • Diagnostic Test: swab

Detailed Description

The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,…) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to the following steps to identify the causative bacteria:

  • Culturing on blood agar, chocolate agar, MacConkey agar and Mannitol salt agar , gram staining and biochemical tests.

  • Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation.

  • Antimicrobial Susceptibility Testing using the Kirby-Bauer disk diffusion method.

Study Design

Study Type:
Observational
Anticipated Enrollment :
100 participants
Observational Model:
Other
Time Perspective:
Cross-Sectional
Official Title:
In Vitro Biofilm Formation and Antibiotic Susceptibility Patterns of Bacteria From Surgical Site Infection After Orthopedic Surgery
Anticipated Study Start Date :
Jan 1, 2022
Anticipated Primary Completion Date :
Jul 1, 2022
Anticipated Study Completion Date :
Jan 1, 2023

Outcome Measures

Primary Outcome Measures

  1. detect biofilm formation [one year]

    The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,…) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation.

Eligibility Criteria

Criteria

Ages Eligible for Study:
N/A and Older
Sexes Eligible for Study:
All
Accepts Healthy Volunteers:
No
Inclusion Criteria:
  • patients with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,…)
Exclusion Criteria:
  • if patient has no orthopedic surgery

Contacts and Locations

Locations

No locations specified.

Sponsors and Collaborators

  • Sohag University

Investigators

  • Principal Investigator: principal investigator, Sohag University

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.
Responsible Party:
Amira Ahmed Talaat, Principal investigator, Sohag University
ClinicalTrials.gov Identifier:
NCT05181163
Other Study ID Numbers:
  • Soh-Med-21-11-03
First Posted:
Jan 6, 2022
Last Update Posted:
Jan 6, 2022
Last Verified:
Dec 1, 2021
Individual Participant Data (IPD) Sharing Statement:
Undecided
Plan to Share IPD:
Undecided
Studies a U.S. FDA-regulated Drug Product:
No
Studies a U.S. FDA-regulated Device Product:
No
Keywords provided by Amira Ahmed Talaat, Principal investigator, Sohag University
biofilm antibiotics

Study Results

No Results Posted as of Jan 6, 2022