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Nitrate and Power Output After Two Beetroot Juice Drinks

Sponsor
Nanyang Technological University (Other)
Overall Status
Recruiting
CT.gov ID
NCT05452421
Collaborator
(none)
24
Enrollment
1
Location
2
Arms
7.1
Anticipated Duration (Months)
3.4
Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The main aim of this study is to determine and compare the optimal time to achieve peak concentration of blood plasma nitrite after ingestion of beetroot juice (BR) and isotonic beetroot juice (ISO-CB), respectively. Optimal timing of drink consumption in relation to exercise can then be prescribed in relation to nitrite appearance in the blood. A randomised controlled trial with participants completing two conditions: (i) BR and (ii) ISO-CB. On each condition participants will consume During each trial, participants will ingest 2 x 285ml of beverage 3 hours prior to performing two jump tests follow by a Wingate test. Salivary NO content will be measured at the start of the morning, 1 hour, 2 hours, 3 hours after consumption of beverage and at the end of the exercise.

Condition or Disease Intervention/Treatment Phase
  • Dietary Supplement: Isotonic carbohydrate beetroot juice drink
  • Dietary Supplement: Beetroot juice drink
 N/A

Detailed Description

  1. The main aim of this study is to determine and compare the optimal time to achieve peak concentration of blood plasma nitrite after ingestion of beetroot juice (BR) and isotonic beetroot juice (ISO-CB), respectively. Optimal timing of drink consumption in relation to exercise can then be prescribed in relation to nitrite appearance in the blood.

  2. A secondary aim of this study is to evaluate the association between blood plasma nitrite concentration and saliva nitrite concentration after ingestion of the two drinks to determine whether blood plasma analysis can be fully or partially substituted with a salivary nitrite test.

  3. A final aim of this study is to investigate the ergogenic effects of ISO-CB compared with BR during a single 30 second cycling sprint bout (Wingate test) and two jumping tests.

A randomised controlled trial recruiting 24 healthy male and female participants. Each participant will complete two conditions: (i) BR and (ii) ISO-CB. On each condition participants will consume During each trial, participants will ingest 2 x 285ml of beverage 3 hours prior to performing two jump tests follow by a Wingate test. In the next trial, participants will undergo the same procedures, but ingest the beverage that they did not take the previous round. The study will be double-blinded study. Blood samples will be taken before supplementation, 2 hours and 3 hours after consuming the beverage. Salivary NO content will be measured at the start of the morning, 1 hour, 2 hours, 3 hours after consumption of beverage and at the end of the exercise. For each trial, participants will also fill out a sensory evaluation form in relation to the drink consumed. It is hypothesized that blood plasma nitrite and saliva nitrite appearance will be earlier with ISO-CB and that participants will have improved peak and mean power when ISO-CB is consumed compared with BR.

Study Design

Study Type:
Interventional
Anticipated Enrollment :
24 participants
Allocation:
Randomized
Intervention Model:
Crossover Assignment
Masking:
Triple (Participant, Investigator, Outcomes Assessor)
Masking Description:
Two drinks similar in colour and texture have been provided by a local manufacturer which are coded. The code is held with a third party not involved in the research. Participants and researchers will be asked to guess which drink has been used at the end of each trial to confirm the effectiveness of the masking procedure.
Primary Purpose:
Other
Official Title:
Plasma and Salivary Nitrate/Nitrite Responses and Power Output Compared After Two Beetroot Juice Drinks
Actual Study Start Date :
Jul 1, 2022
Anticipated Primary Completion Date :
Feb 1, 2023
Anticipated Study Completion Date :
Feb 1, 2023

Arms and Interventions

Arm Intervention/Treatment
Experimental: Group A

Dietary Supplement: Isotonic carbohydrate beetroot juice drink
An isotonic carbohydrate beetroot juice drink containing 141 mg nitrate per 100 ml (12.9 mmol). Total of 570 ml serving in two doses of 285 ml over one morning. Contains 6.2 g carbohydrate per 100 ml. Osmolality 304-310 mOsm/kg.
Other Names:
  • ISO-CB

    • Dietary Supplement: Beetroot juice drink
    A beetroot juice drink containing 141 mg nitrate per 100 ml (12.9 mmol). Total of 570 ml serving in two doses of 285 ml over one morning. Contains 6.1 g carbohydrate per 100 ml. Osmolality 390-410 mOsm/kg.
    Other Names:
  • BR

    		•
    Experimental: Group B

    Dietary Supplement: Isotonic carbohydrate beetroot juice drink
    An isotonic carbohydrate beetroot juice drink containing 141 mg nitrate per 100 ml (12.9 mmol). Total of 570 ml serving in two doses of 285 ml over one morning. Contains 6.2 g carbohydrate per 100 ml. Osmolality 304-310 mOsm/kg.
    Other Names:
  • ISO-CB

    • Dietary Supplement: Beetroot juice drink
    A beetroot juice drink containing 141 mg nitrate per 100 ml (12.9 mmol). Total of 570 ml serving in two doses of 285 ml over one morning. Contains 6.1 g carbohydrate per 100 ml. Osmolality 390-410 mOsm/kg.
    Other Names:
  • BR

    		•

    Outcome Measures

    Primary Outcome Measures

    1. Blood plasma nitrite [3 hours]

      Peak concentration of blood plasma nitrite

    Secondary Outcome Measures

    1. Salivary nitrite [3 hours]

      Salivary nitrite concentration

    2. Power output [30 seconds]

      Peak and mean power output

    3. Jump height and distance [Two jumps averaged]

      Jump height and distance

    Eligibility Criteria

    Criteria

    Ages Eligible for Study:
    21 Years to 35 Years
    Sexes Eligible for Study:
    All
    Accepts Healthy Volunteers:
    Yes
    Inclusion Criteria:

    1. Age: 21-35 years.

    2. Healthy and injury-free: defined as no positive (Yes) answers to the Physical Activity Readiness Questionnaire (PAR-Q+) and not reporting any other health issues that may be affected by exercise.

    3. Blood pressure: <130/90 mmHg.

    4. Physically active: Engaging in a minimum of 150 minutes of moderate-intensity exercise or 75 minutes of vigorous-intensity physical activity each week determined via self-report Global Physical Activity Questionnaire (GPAQ).

    5. Body mass index (BMI): 18.5-25.0 kg/m2.

    6. Non-smoker.

    Exclusion Criteria:

    1. Any allergies to beetroot or isotonic drink.

    2. Asthma.

    3. Any report of personal health issues that may compromise the individual's ability to undertake exercise safely.

    Contacts and Locations

    Locations

    Site City State Country Postal Code
    1 Human Bioenergetics Laboratory, National Institute of Education, Nanyang Technological University Singapore Singapore 637616

    Sponsors and Collaborators

    • Nanyang Technological University

    Investigators

    None specified.

    Study Documents (Full-Text)

    None provided.

    More Information

    Publications

    None provided.
    Responsible Party:
    Stephen Burns, Associate Professor, Nanyang Technological University
    ClinicalTrials.gov Identifier:
    NCT05452421
    Other Study ID Numbers:
    • IRB-2022-149
    First Posted:
    Jul 11, 2022
    Last Update Posted:
    Jul 11, 2022
    Last Verified:
    Jul 1, 2022
    Individual Participant Data (IPD) Sharing Statement:
    No
    Plan to Share IPD:
    No
    Studies a U.S. FDA-regulated Drug Product:
    No
    Studies a U.S. FDA-regulated Device Product:
    No

    Study Results

    No Results Posted as of Jul 11, 2022