Brain Death: Inflammatory Response, Apoptosis, and Endotoxin Tolerance

Sponsor

Delafontaine Hospital (Other)

Overall Status

Completed

CT.gov ID

NCT00615225

Collaborator

(none)

Locations

Duration (Months)

Study Details

Study Description

Brief Summary

We hypothesized that brain death is associated with an early systemic inflammatory response, possibly combined with activation of apoptotic cell death, two events that may contribute to induce rapid organ dysfunction. In this study of brain-dead donors and controls, we assayed plasma cytokines and soluble factors, investigated plasma endotoxin levels as a triggering factor for inflammation, measured ex vivo cytokine production by blood leukocytes to determine whether immunosuppression occurred after brain death, and examined skeletal muscle biopsies to look for evidence of inflammation and increased apoptosis in peripheral tissue.

Condition or Disease	Intervention/Treatment	Phase
Brain Death	Procedure: Muscle biopsy

Study Design

Study Type:

Observational

Official Title:

Observational Study on the Systemic Inflammatory Response During Brain Death.

Study Start Date :

Jan 1, 2000

Actual Primary Completion Date :

Nov 1, 2007

Actual Study Completion Date :

Dec 1, 2007

Arms and Interventions

Arm	Intervention/Treatment
Brain dead patients All patients meeting criteria for brain death	Procedure: Muscle biopsy Muscle biopsies were taken through the operation site from the vastus lateralis thigh muscle at the beginning of surgery
Healthy control Any healthy volunteers accepting to give some blood
Volunteers having hip surgery Patients undergoing hip surgery for degenerative non-inflammatory hip disease	Procedure: Muscle biopsy Muscle biopsies were taken through the operation site from the vastus lateralis thigh muscle at the beginning of surgery

Arm

Intervention/Treatment

Brain dead patients

All patients meeting criteria for brain death

Procedure: Muscle biopsy

Muscle biopsies were taken through the operation site from the vastus lateralis thigh muscle at the beginning of surgery

Healthy control

Any healthy volunteers accepting to give some blood

Volunteers having hip surgery

Patients undergoing hip surgery for degenerative non-inflammatory hip disease

Procedure: Muscle biopsy

Muscle biopsies were taken through the operation site from the vastus lateralis thigh muscle at the beginning of surgery