A Phase 1b Trial of ATRC-101 in Adults With Advanced Solid Malignancies

Sponsor

Atreca, Inc. (Industry)

Overall Status

Recruiting

CT.gov ID

NCT04244552

Collaborator

(none)

240

Enrollment

Locations

Arms

60.6

Anticipated Duration (Months)

18.5

Patients Per Site

0.3

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

ATRC-101-A01 is a Phase 1b, open-label dose escalation and expansion trial of ATRC-101, an engineered fully human immunoglobulin G, subclass 1 (IgG1) antibody derived from a naturally occurring human antibody. The safety, tolerability, PK, and biological activity of ATRC-101 will be characterized when administered every two weeks (Q2W) or every 3 weeks (Q3W) as a monotherapy or in combination with other anticancer agents.

Condition or Disease	Intervention/Treatment	Phase
Breast Cancer Colorectal Cancer Ovarian Cancer Non-Small Cell Lung Cancer Acral Lentiginous Melanoma Head and Neck Squamous Cell Carcinoma Hepatocellular Carcinoma Esophageal Squamous Cell Carcinoma Urothelial Carcinoma DMMR Colorectal Cancer MSI-H Colorectal Cancer Melanoma Platinum-Resistant Primary Peritoneal Carcinoma Platinum-Resistant Fallopian Tube Carcinoma Platinum-Resistant Epithelial Ovarian Cancer Triple Negative Breast Cancer	Biological: ATRC-101 Biological: Pembrolizumab Drug: Pegylated liposomal doxorubicin (PLD)	Phase 1

Detailed Description

For the monotherapy cohorts, including the efficacy expansion cohorts, enrollment is restricted to adults with inoperable, locally advanced or metastatic breast cancer, NSCLC, CRC, ovarian cancer, and acral melanoma, which are all tumor types that have demonstrated ATRC-101 immunoreactivity on at least 50% of tested commercially procured archival specimens.

For the pembrolizumab combination therapy cohort, enrollment is restricted to adults with inoperable, locally advanced or metastatic NSCLC, CRC (only MSI-H or dMMR), melanoma (with the exception of uveal melanoma), HCC, HNSCC, ESCC, UC or TNBC, that have been treated with anti-PD-1 or anti-PD-L1 therapy and have progressed radiographically or have achieved stable disease for a minimum of two months and who, in the judgment of their treating physicians, could benefit from a combination of ATRC 101 and pembrolizumab.

For the PLD combination therapy cohort, enrollment is restricted to adult females with inoperable, locally advanced or metastatic high-grade serous epithelial ovarian, fallopian tube, or primary peritoneal cancer that is platinum resistant, defined as progression during or within 6 months of the last dose of platinum-based chemotherapy OR breast cancer that is refractory to other standard therapies.

For target-enriched expansion cohorts, enrollment will be limited to participants with pretreatment tumor biopsies demonstrating ATRC-101 target expression above a predefined threshold by IHC at a central laboratory. Target-enriched cohorts will include the same tumor types as the respective unenriched cohorts.

Study Design

Study Type:

Interventional

Anticipated Enrollment :

240 participants

Allocation:

Non-Randomized

Intervention Model:

Sequential Assignment

Masking:

None (Open Label)

Primary Purpose:

Treatment

Official Title:

A Phase 1b Dose Escalation and Expansion Trial to Investigate the Safety, Tolerability, Pharmacokinetics, and Biological Activity of ATRC-101 as Monotherapy and in Combination With Other Anticancer Agents in Adults With Advanced Solid Malignancies

Actual Study Start Date :

Feb 11, 2020

Anticipated Primary Completion Date :

Dec 1, 2024

Anticipated Study Completion Date :

Mar 1, 2025

Arms and Interventions

Arm	Intervention/Treatment
Experimental: ATRC-101 Q3W	Biological: ATRC-101 ATRC-101 is an engineered, fully-human IgG1 antibody derived from a naturally-occurring human antibody.
Experimental: ATRC-101 Q2W	Biological: ATRC-101 ATRC-101 is an engineered, fully-human IgG1 antibody derived from a naturally-occurring human antibody.
Experimental: ATRC-101 Q3W + Pembrolizumab Pembrolizumab 200mg IV Q3W or 400mg IV Q6W	Biological: ATRC-101 ATRC-101 is an engineered, fully-human IgG1 antibody derived from a naturally-occurring human antibody. Biological: Pembrolizumab Pembrolizumab (Keytruda®) is a humanized immunoglobulin G4 monoclonal antibody with high specificity of binding to the programmed cell death 1 (PD-1) receptor, thus inhibiting its interaction with programmed cell death ligand 1 (PD-L1) and programmed cell death ligand 2 (PD-L2).
Experimental: ATRC-101 Q2W + Pegylated liposomal doxorubicin (PLD) PLD 40mg/m^2 IV Run-in period of 28 days, and then 40mg/m^2 IV Q4W	Biological: ATRC-101 ATRC-101 is an engineered, fully-human IgG1 antibody derived from a naturally-occurring human antibody. Drug: Pegylated liposomal doxorubicin (PLD) Pegylated liposomal doxorubicin (PLD) is doxorubicin hydrochloride (HCl), an anthracycline topoisomerase inhibitor that can bind DNA and inhibit nucleic acid synthesis. PLD is doxorubicin HCL encapsulated in liposomes formulated with surface-bound methoxypolyethylene glycol Other Names: DOXIL®, PLD

Outcome Measures

Primary Outcome Measures

Incidence of DLTs, treatment emergent adverse events (TEAEs), and changes in safety parameters [24 months]

Secondary Outcome Measures

Maximum plasma concentration (Cmax) of ATRC-101 [24 months]
Elimination half-life (t1/2) of ATRC-101 [24 months]
Area under the plasma concentration-time curve from zero to the last measurable concentration [AUC(0-t)] of ATRC-101 [24 months]
Incidence of anti-drug antibodies (ADAs) and ATRC-101 neutralizing antibodies [24 months]
Overall Response Rate (ORR), defined as the proportion of participants with a CR or a PR on two consecutive occasions > 4 weeks apart, according to Response Evaluation Criteria in Solid Tumors (RECIST) v1.1 [24 months]
Enumeration of tumor-infiltrating CD8+ lymphocytes (TILs) in tumor biopsy specimens at baseline and during treatment [24 months]
Distribution of tumor-infiltrating CD8+ lymphocytes (TILs) in tumor biopsy specimens at baseline and during treatment [24 months]
For the PLD Combination Therapy Cohort: ATRC-101 immunoreactivity in tumor biopsies at baseline and during treatment [24 months]
For the Target-Enriched Expansion Cohorts: IHC analysis (H-score, percentage of target positive cells, or maximum staining intensity) [24 months]

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years and Older

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria

Confirmed diagnosis of:
For the monotherapy cohorts: Inoperable, locally advanced or metastatic breast cancer, NSCLC, CRC, ovarian cancer, or acral melanoma that is refractory to standard therapy or for which no standard therapy exists. Participants who are considered intolerant of or ineligible for standard therapy(ies), as well as participants who have been offered but refused standard therapy(ies), may also be eligible.
For the pembrolizumab combination therapy cohort: Inoperable, locally advanced or metastatic NSCLC, CRC (only MSI-H or dMMR), melanoma (with the exception of uveal melanoma), HCC, HNSCC, ESCC,UC, or TNBC with prior or ongoing anti-PD-1 or anti-PD-L1 treatment and have progressed radiographically or have achieved stable disease for a minimum of two months and who, in the judgment of their treating physicians, could benefit from a combination of ATRC-101 and pembrolizumab . The anti-PD-1/anti-PD-L1 therapy must be FDA approved for their cancer indication at the time of screening. Patients with the tumor types eligible for monotherapy that are TMB-H, MSI-H or dMMR and have had an unsatisfactory response to anti-PD-1/anti-PD-L1 therapy may enroll for the pembrolizumab combination, and additional indications that have been identified as ATRC-101 target positive and are FDA approved for pembrolizumab may be added following discussion with the study Medical Monitor.

Individuals with BRAF mutant melanoma must have received BRAF inhibitors alone or in combination with a MEK inhibitor, if indicated.
Individuals with NSCLC should have received platinum-based therapy unless contraindicated

For the PLD combination therapy cohort: Inoperable, locally advanced or metastatic high-grade serous epithelial ovarian, fallopian tube, or primary peritoneal cancer that is platinum resistant, defined as progression during or within 6 months of the last dose of platinum-based chemotherapy OR breast cancer that is refractory to other standard therapies.
For ATRC-101 target-enriched expansion cohort(s): Target-enriched cohort(s) will include the same tumor types as the respective unenriched cohort(s).
Measurable disease based on RECIST 1.1, as assessed by the local site investigator/radiologist. As per RECIST, lesions situated in an area treated with radiation or other loco-regional therapy are considered measurable only if progression has been demonstrated in such lesions following loco-regional therapy.
Eastern Cooperative Oncology Group (ECOG) performance status of 0-1
Adequate organ and marrow function (i.e., without chronic, ongoing growth factor or transfusion support) at Screening as defined by the following laboratory parameters via central laboratory results:
Absolute neutrophil count (ANC)

For monotherapy and pembrolizumab combination therapy cohorts: ≥ 1000/µL
For PLD combination therapy cohort: ≥ 1500/µL

Absolute lymphocyte count (ALC) ≥ 500/µL
Platelet count ≥ 75,000/µL
Hemoglobin ≥ 9.0 g/dL
PT/INR, aPTT ≤ 1.5 x ULN unless participant is receiving a stable dose of therapeutic anticoagulation
Albumin ≥ 3.0 g/dL
Creatinine clearance or eGFR ≥ 30 mL/min as estimated by the Cockcroft-Gault equation
AST/ALT ≤ 2 x ULN. If documented liver metastases, then ≤ 5X ULN
Bilirubin

For monotherapy and pembrolizumab combination therapy cohorts: ≤ 2 x ULN; or bilirubin ≤ 3 x ULN if due to Gilbert's or Crigler-Najjar disease
For PLD combination therapy cohort: ≤ ULN

Available representative tumor specimens in paraffin blocks (preferred) or ≥ 20 unstained slides (serial sections), with an associated pathology report, obtained after last systemic anticancer therapy and within 60 days prior to the planned first dose of investigational product (Cycle 1-Day 1). If fewer than 20 unstained slides are available, a discussion with the Medical Monitor is required prior to enrollment. If an archived sample is not available, participant must have a tumor that is amenable to biopsy without unacceptable risk of a major procedural complication and consent to have a tumor biopsy. Tumor lesions used for biopsy should not be lesions used as RECIST 1.1 target lesions unless there are no other lesions suitable for biopsy. If a RECIST target lesion is used for biopsy, the lesion must be ≥ 2 cm in longest diameter. Fine-needle aspirates, cell pellets from pleural effusions, ascites, and bone metastases are not acceptable.

For the pembrolizumab combination therapy cohort: A biopsy collected within 60 days of the planned first dose of investigational product while the participant is receiving anti-PD-1/anti-PD-L1 therapy is acceptable.

Women of childbearing potential (WOCBP) and fertile males with partners who are WOCBP must use highly effective contraception (per CTFG 2014) from first dose and through 90 days after final dose of investigational product
Willing and able to provide written informed consent and able to comply with all trial procedures
For ATRC-101 target-enriched expansion cohort(s): Enrollment will be restricted to participants with an archival tumor biopsy (a pretreatment tumor biopsy obtained before consent to participate in the current study was signed) and the biopsy demonstrating ATRC-101 target expression above a predefined threshold by IHC at a central laboratory inclusion (3-5 unstained slides, serial sections).

If multiple archived tumor biopsies are available, the site should prioritize the most recent biopsy. If multiple biopsies were performed at the most recent time point, the clinical sites should prioritize lesions distinct from the anticipated screening biopsy anatomical site.
All participants, including those enrolled in target-enriched expansion cohorts, will undergo a tumor biopsy during the screening window (screening biopsy). A tumor biopsy obtained before screening but within 60 days prior to planned Cycle 1-Day 1 may suffice as a screening biopsy provided no anti-cancer therapy was administered after the biopsy.

Exclusion Criteria

Individuals who meet any of the following criteria are not eligible to participate in this trial:

Disease that is suitable for local therapy administered with curative intent.
Malignant disease other than the malignancy to be investigated in this trial within the last 5 years with the exception of:

basal or squamous cell carcinoma of the skin OR
curatively treated in situ disease OR
localized (non-metastatic) prostate cancer treated with curative intent and undetectable PSA

Autoimmune disease requiring systemic treatment (e.g., with disease modifying agents, corticosteroids, or immunosuppressive drugs) in the past 2 years. Hormone replacement therapy (e.g., insulin, thyroxine, and replacement-dose hydrocortisone) is not considered systemic treatment.
Active or prior paraneoplastic neurologic disorder of the central nervous system (CNS) or history of leptomeningeal disease
Prior allogenic hematopoietic or solid organ transplant
Clinically significant cardiovascular disease, e.g., cerebral vascular accident/stroke or myocardial infarction, within 6 months prior to first dose of investigational product, unstable angina, congestive heart failure (New York Heart Association ≥ Class III), or unstable cardiac arrhythmia requiring medication
For the PLD Combination Therapy Cohort:

Any history of documented congestive heart failure (CHF), arrhythmia, or uncontrolled hypertension (systolic BP > 200 mmHg or diastolic BP > 100 mmHg)
Left ventricular ejection fraction measure by echocardiography or multigated radionuclide acquisition (MUGA) below normal limits for the institution

Presence of active, symptomatic, or untreated CNS metastasis; or CNS metastasis that requires local directed therapy or increasing doses of corticosteroids within the 2 weeks prior to the planned first dose of investigational product. Individuals with treated and/or asymptomatic CNS disease may be enrolled if neurologically stable over the prior 2 weeks (and after consultation with the Medical Monitor) provided there is measurable disease (RECIST 1.1) outside of the CNS and there is no ongoing requirement for corticosteroids to manage the disease
HIV infection with an AIDS-defining opportunistic infection within the past 12 months or with a CD4+ T cell count <350/µL
Hepatitis B surface antigen (HbsAg) positive OR Hepatitis B core antibody (anti-HBc or HBcAb) positive and HBV viral load above the lower limit of quantification
Hepatitis C antibody positive with HCV viral load greater than or equal to the lower limit of quantification
Infection requiring intravenous antibacterial, antiviral, or antifungal therapy within 2 weeks prior to the planned first dose of investigational product
Ongoing ≥ Grade 2 toxicity(ies) due to a previously administered anticancer agent with the following exceptions:

Grade 2 neuropathy or alopecia
For the monotherapy cohorts: Grade 2 immune-related endocrinopathy attributed to a checkpoint inhibitor and controlled with hormone replacement alone

Treatment with biological agents (including monoclonal antibodies and cytokines) within 28 days of the planned first dose of investigational product with the following exception:

a.For the pembrolizumab combination therapy cohort: -Anti-PD-1/anti-PD-L1 treatment within 28 days of the planned first dose of investigational product.

Treatment with radiation, chemotherapy or anticancer small molecule therapy within 14 days or 5 half-lives (whichever is longer) prior to the first dose of investigational product. Treatment with nitrosoureas or mitomycin C require a 42-day washout prior to the planned first dose of investigational product
For the PLD combination therapy cohort:

Prior treatment with PLD
Prior treatment with doxorubicin or other anthracycline at cumulative doses greater than 300mg/m2.
Anthracycline equivalent doses: 1 mg Doxorubicin = 1.8 mg Epirubicin = 0.3 mg Mitoxantrone = 0.25 mg Idarubicin
Anthracyclines or anti-HER2 agents, if last dose was administered < 1 year before enrollment
Prior mediastinal irradiation > 3500 cGY

Receipt of any investigational drug or device not otherwise specified above within 28 days or 5 half-lives (whichever is longer) prior to the planned first dose of investigational product
Pregnant or breastfeeding; negative pregnancy status in WOCBP must be confirmed by serum pregnancy test at Screening
History of ≥ Grade 3 infusion-related reaction associated with antibody administration, or:

For the monotherapy cohorts: Known allergy/intolerance to ATRC-101 or its excipients
For the pembrolizumab combination therapy cohort: Known allergy/intolerance to ATRC-101, anti-PD-1 or anti-PD-L1, , or their excipients
For the PLD combination therapy cohort: Known allergy/intolerance to ATRC-101, doxorubicin, or to the excipients of ATRC-101 or PLD

Major surgery or significant traumatic injury occurring within 28 days prior to the planned first dose of investigational product. If major surgery occurred > 28 days prior to Cycle 1-Day 1, individual must have recovered adequately from the toxicity and/or complications from the intervention prior to Cycle 1-Day 1
Prior treatment with ATRC-101
Intercurrent illness that is either life-threatening or of clinical significance such that it might limit compliance with trial requirements, or in the Investigator's assessment would place the participant at an unacceptable risk for participation. Uncontrolled pleural effusion, pericardial effusion or ascites requiring recurrent drainage procedures is exclusionary
Receipt of a live, attenuated vaccine within 28 days of planned Cycle 1-Day 1. Examples of live vaccines include, but are not limited to, the following: measles, mumps, rubella, varicella/zoster (chicken pox), yellow fever, rabies, Bacillus Calmette-Guerin (BCG), and typhoid vaccine. Seasonal influenza vaccines for injection are generally inactivated flu vaccines and are allowed. Intranasal influenza vaccines (e.g. FluMist) are live attenuated vaccines and are not allowed. Replication-incompetent viral, mRNA, subunit, conjugate, and toxoid vaccines are allowed.
COVID-19 (SARS-CoV-2) pandemic consideration: The COVID-19 vaccination should not be delayed; however, caution should be exercised when combining vaccination with cancer therapy, particularly immunotherapy. The COVID-19 vaccines can cause transient lymphadenopathy and potentially impact assessment of disease burden during screening and/or on-study. To allow correct interpretation and reduce equivocal findings, investigators should discuss with the participant appropriate timing and selection of anatomic site of vaccination (alternative) with respect to imaging scans (MRI or CT). Screening scans should be acquired before the first dose of a COVID-19 vaccine or 4-6 weeks after the second dose of the vaccine. Because cancer patients are considered vulnerable population, after COVID-19 vaccination there is also a potential for heightened immune related adverse events including CRS. Investigators should use judgement when evaluating and managing potentially overlapping adverse events and in establishing causality with the study treatment (Schönrich and Raftery 2019; Desai et al. 2021; Edmonds et al. 2021; Indini et al. 2021)

For the pembrolizumab combination therapy cohort ONLY:

Experienced ≥ Grade 3 immune related adverse events while on immunotherapy prior to enrollment
Have not recovered from Grade 2 immune related adverse events attributed to immunotherapy to Grade 1 or baseline prior to enrollment.
NSCLC with epidermal growth factor receptor (EGFR) or anaplastic lymphoma receptor tyrosine kinase (ALK) genomic tumor alterations
Isolated intracranial relapse
Interstitial lung disease or active, non-infectious pneumonitis
Signs and symptoms consistent with clinically significant, decreased pulmonary function: (1) blood saturation oxygen level (SpO2) < 90% at rest on room air; (2) dyspnea at rest (CTCAE v. 5.0 ≥ Grade 3) or supplemental oxygen used to maintain adequate oxygenation within 14 days prior to the planned first dose of investigational product
Ongoing immune-related toxicity or immune-related toxicity requiring systemic corticosteroids for 30 or more consecutive days for a prior immune related adverse event before initiation of study treatment

For the PLD combination therapy cohort ONLY:

•Prior drug-induced cardiotoxicity, defined as a sustained decrease in the ejection fraction (EF) of > 15%.

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Mayo Clinic	Phoenix	Arizona	United States	85054
2	The University of Arizona Cancer Center	Tucson	Arizona	United States	85719
3	City of Hope	Duarte	California	United States	91010
4	University of California, Los Angeles Hematology/Oncology	Los Angeles	California	United States	90095
5	Georgetown University Medical Center	Washington	District of Columbia	United States	20007
6	Mayo Clinic	Jacksonville	Florida	United States	32224
7	University of Miami Hospital - Sylvester Comprehensive Cancer Center	Miami	Florida	United States	33136
8	Massachusetts General Hospital	Boston	Massachusetts	United States	02114
9	Mayo Clinic	Rochester	Minnesota	United States	55902
10	Icahn School of Medicine at Mount Sinai	New York	New York	United States	10029
11	Carolina BioOncology Institute	Huntersville	North Carolina	United States	28078
12	Stephenson Cancer Center, University of Oklahoma Health Sciences Center	Oklahoma City	Oklahoma	United States	73104
13	Vanderbilt University Medical Center	Nashville	Tennessee	United States	37232