Analysis of CA-MRSA Transmission: An ED Population Sampling Strategy
Study Details
Study Description
Brief Summary
Given that the Emergency Department (ED) has become the entry way for large populations of patients into the health care system, a strategy of sampling MRSA isolates in ED populations and merging this information with patient-level data may present a window to hypothesize and investigate CA-MRSA transmission within the community and its impact on hospital-acquired infections.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
Prospective cross-sectional study involving 500 patients enrolled over a one year period at the UFHealth Shands Hospital's Adult and Pediatric Emergency Department. The collected information will serve as pilot data for a future large comprehensive multi-site study.
Patients will have a wound culture and a nasal swab obtained as part of the study, which will be assessed for MRSA isolates using next-generation whole genome sequencing. The principal investigator or PI designee will also survey participants and review hospital records.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Acute Abscess Group Adults and pediatric patients presenting to the UFHealth Shands Emergency Department with evidence of an acute abscess, or skin/soft tissue infection, which can be sampled for culture and sensitivity testing will be recruited. |
Other: Acute Abscess Group
Samples collected for culture and sensitivity testing depending on acute abscess, or skin/soft tissue infection.
|
Outcome Measures
Primary Outcome Measures
- Strain Relatedness Identification Through Phylogenetic Analysis [1 Hour]
Molecular (spa-typing) and genomic (WGS) relatedness of MRSA strains in isolates in ED populations presenting with SSTIs.
Secondary Outcome Measures
- Strain Transmission Through Analysis of Genetic Clustering [1 year]
Phlyodynamic analysis of MRSA transmission course by strain
- Patient Level Characteristics of Skin and Soft Tissue Infection (SSTI) Presentation [6 Months]
To determine patient-level characteristics, including social and medical history, associated with a presentation related to SSTIs.
- Pediatric and Adult Population Presentation Characteristics in Skin and Soft Tissue Infection (SSTI) [6 Months]
Compare patient-level characteristics and phylogenetic clustering between pediatric and adult patients presenting with SSTIs.
Eligibility Criteria
Criteria
Inclusion Criteria:
-
Patient or Legally Authorized Representative (LAR) must have voluntarily signed an Institutional Review Board-approved informed consent form before initiation of any study procedures
-
Patient presents with an acute abscess or a non-post-operative skin/soft tissue infection
-
Patient presents through the UFHealth Shands Emergency Department
Exclusion Criteria:
-
Patients who are employed by UFHealth and provide direct patient care
-
Patients who have previously been enrolled in the study
-
Patients who are not suitable for the study in the opinion of the investigator
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | UF Health | Gainesville | Florida | United States | 32608 |
Sponsors and Collaborators
- University of Florida
Investigators
- Principal Investigator: Joseph A Tyndall, MD, University of Florida
Study Documents (Full-Text)
None provided.More Information
Publications
- Burton DC, Edwards JR, Horan TC, Jernigan JA, Fridkin SK. Methicillin-resistant Staphylococcus aureus central line-associated bloodstream infections in US intensive care units, 1997-2007. JAMA. 2009 Feb 18;301(7):727-36. doi: 10.1001/jama.2009.153.
- Climo MW. Decreasing MRSA infections: an end met by unclear means. JAMA. 2009 Feb 18;301(7):772-3. doi: 10.1001/jama.2009.149.
- Park SH, Park C, Yoo JH, Choi SM, Choi JH, Shin HH, Lee DG, Lee S, Kim J, Choi SE, Kwon YM, Shin WS. Emergence of community-associated methicillin-resistant Staphylococcus aureus strains as a cause of healthcare-associated bloodstream infections in Korea. Infect Control Hosp Epidemiol. 2009 Feb;30(2):146-55. doi: 10.1086/593953.
- Prosperi M, Veras N, Azarian T, Rathore M, Nolan D, Rand K, Cook RL, Johnson J, Morris JG Jr, Salemi M. Molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus in the genomic era: a cross-sectional study. Sci Rep. 2013;3:1902. doi: 10.1038/srep01902.
- Wu D, Wang Q, Yang Y, Geng W, Wang Q, Yu S, Yao K, Yuan L, Shen X. Epidemiology and molecular characteristics of community-associated methicillin-resistant and methicillin-susceptible Staphylococcus aureus from skin/soft tissue infections in a children's hospital in Beijing, China. Diagn Microbiol Infect Dis. 2010 May;67(1):1-8. doi: 10.1016/j.diagmicrobio.2009.12.006. Epub 2010 Mar 12.
- IRB201400426