Caffeine Supplementation, Resistance Training and Blood Antioxidant Status

Sponsor

The Jerzy Kukuczka Academy of Physical Education in Katowice (Other)

Overall Status

Recruiting

CT.gov ID

NCT05230303

Collaborator

(none)

Enrollment

Location

Arms

7.6

Anticipated Duration (Months)

2.6

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The purpose of this study is to verify the effect of acute caffeine intake on resistance training volume and blood antioxidant status, in a randomised, double-blind, placebo-controlled crossover trial.

Condition or Disease	Intervention/Treatment	Phase
Caffeine	Dietary Supplement: Caffeine supplementation Dietary Supplement: Placebo treatment	N/A

Detailed Description

Caffeine is one of the most commonly consumed psychoactive substances in the world. Several previous studies confirmed that caffeine improves several aspects of exercise performance, including resistance training outcomes. Interestingly, caffeine and its catabolic products, theobromine and xanthine, exhibit antioxidant and prooxidant properties. Additionally, resistance training promotes the generation of reactive oxygen species, which are important for the cellular adaptation process.

Hovewer, to the best of our knowledge, there is no information as to how caffeine combined with resistance training affects blood antioxidant status. Therefore, the aim of this study was to examine acute effects of caffeine supplementation on resistance training volume and blood antioxidant status in resistance trained men.

Study Design

Study Type:

Interventional

Anticipated Enrollment :

20 participants

Allocation:

Randomized

Intervention Model:

Crossover Assignment

Masking:

Triple (Participant, Investigator, Outcomes Assessor)

Primary Purpose:

Treatment

Official Title:

Acute Effects of Caffeine Supplementation on Resistance Training Volume and Blood Antioxidant Status in Resitance Trained Men

Actual Study Start Date :

Jan 12, 2022

Anticipated Primary Completion Date :

Sep 1, 2022

Anticipated Study Completion Date :

Sep 1, 2022

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Caffeine supplementation Group taking 3 mg/kg of caffeine	Dietary Supplement: Caffeine supplementation Two identical experimental sessions with a one-week interval between sessions (to allow complete recovery and ensure substance wash-out) will be taken. During the experimental sessions, participants will ingest caffeine in dose of 3 mg/kg of body mass. Caffeine will be provided in capsules containing the individual amount of caffeine and will be administered orally 60 min before the onset of the exercise protocol.
Placebo Comparator: Placebo treatment Group taking placebo	Dietary Supplement: Placebo treatment The experimental procedure for each particpant will include placebo supplementation. The manufacturer of the caffeine capsules will also prepare identical placebo capsules filled out with a microcrystalline cellulose. Placebo will be administered orally 60 min before the onset of the exercise protocol.

Arm

Intervention/Treatment

Experimental: Caffeine supplementation

Group taking 3 mg/kg of caffeine

Dietary Supplement: Caffeine supplementation

Two identical experimental sessions with a one-week interval between sessions (to allow complete recovery and ensure substance wash-out) will be taken. During the experimental sessions, participants will ingest caffeine in dose of 3 mg/kg of body mass. Caffeine will be provided in capsules containing the individual amount of caffeine and will be administered orally 60 min before the onset of the exercise protocol.

Placebo Comparator: Placebo treatment

Group taking placebo

Dietary Supplement: Placebo treatment

The experimental procedure for each particpant will include placebo supplementation. The manufacturer of the caffeine capsules will also prepare identical placebo capsules filled out with a microcrystalline cellulose. Placebo will be administered orally 60 min before the onset of the exercise protocol.

Outcome Measures

Primary Outcome Measures

Change the levels of oxidative stress markers [Day 1, Day 2]
Serum will be obtained for TOS/TOC (total oxidative stress), MDA (malondialdehyde), oncentration of TAS (total antioxidant status), activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx); concentartion of GSH (reduced glutathione) and UA (uric acid)
Changes in markers of muscle damage [Day 1, Day 2]
Serum will be obtained for activity of creatine kinase (CK) and lactate dehydrogenase (LDH), concentration of myoglobin (Mb)
Changes in Inflammation markers [Day 1, Day 2]
Serum will be obtained for level of interleukin 6, TNF alfa (tumor matrix factor), CRP (C-reactive protein)
Changes in markers of liver function [Day 1, Day 2]
Serum will be obtained for activity of ASPAT (aspartate aminotransferase), ALAT (alanine aminotransferase) and gamma glutamyltransferase (GGT)

Secondary Outcome Measures

Changes in resistance training volume [During caffeine and placebo treatment]
Changes in rate of perceived exertion after resistance training [Immediately after exercise protocol during caffeine and placebo treatment]
Changes in heart rate during resistance training [During caffeine and placebo treatment]
Changes in muscle soreness [prior to exercise, and 24, 48, 72, 96 and 120 hours post exercise.]

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 45 Years

Sexes Eligible for Study:

Male

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

written consent to participate,
medical permission by physician
"resistance-trained," defined as having a minimum of two years of resistance training experience
minimum of 2 workout sessions per week in the last 6 months

Exclusion Criteria:

neuromuscular or musculoskeletal disorders
current injury
using any medications, dietary supplements or ergogenic aids which could potentially affect the study outcomes
a positive smoking status
potential allergy to caffeine

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	The Jerzy Kukuczka Academy of Physical Education	Katowice		Poland	40-065

Sponsors and Collaborators

The Jerzy Kukuczka Academy of Physical Education in Katowice

Investigators

Study Director: Adam Zając, Profesor, The Jerzy Kukuczka Academy of Physical Education, Katowice, Poland

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

Aleksandra Filip-Stachnik, PhD, The Jerzy Kukuczka Academy of Physical Education in Katowice

ClinicalTrials.gov Identifier:

NCT05230303

Other Study ID Numbers:

01_2021

First Posted:

Feb 8, 2022

Last Update Posted:

Feb 8, 2022

Last Verified:

Jan 1, 2022

Individual Participant Data (IPD) Sharing Statement:

Plan to Share IPD:

Studies a U.S. FDA-regulated Drug Product:

Studies a U.S. FDA-regulated Device Product:

Keywords provided by Aleksandra Filip-Stachnik, PhD, The Jerzy Kukuczka Academy of Physical Education in Katowice

sports performance, resistance training, nutrition, athlete

Additional relevant MeSH terms:

Caffeine

Study Results

No Results Posted as of Feb 8, 2022