CADENCE: Canadian Study of Arterial Inflammation in Patients With Diabetes and Vascular Events: EvaluatioN of Colchicine

Sponsor

Ottawa Heart Institute Research Corporation (Other)

Overall Status

Recruiting

CT.gov ID

NCT04181996

Collaborator

Canadian Institutes of Health Research (CIHR) (Other)

115

Enrollment

Location

Arms

Anticipated Duration (Months)

2.8

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Cardiovascular Disease (CVD) is a leading cause of death in the developed world. Atherosclerosis causes plaques in the blood vessels and is a common form of CVD. Inflammation is now recognized as a major cause of atherosclerosis. Therapies that target inflammation are being examined as a potential treatment option. Imaging to detect inflammation may be a solution to understand mechanisms and to optimize patient selection and outcomes for these drugs. Fluorodeoxyglucose (FDG) PET imaging can detect inflammation in the plaque and identify patients vulnerable to plaque rupture which cause events such as myocardial infarctions (MI) and strokes. The primary objective of this proposal(CADENCE) is to determine if the drug colchicine has an effect on plaque inflammation in patients at high risk for events (patients with diabetes or pre-diabetes and recent myocardial infarction, stroke or transient ischemic attacks (TIAs)). This mechanistic and proof-of-concept study will set the stage for future studies that will determine if inflammation imaging can be integrated into clinical practice to personalize decisions for anti-inflammation therapies.

Condition or Disease	Intervention/Treatment	Phase
Cardiovascular Diseases Atherosclerosis Inflammation Diabetes	Drug: Colchicine Oral Product Drug: Placebo oral capsule	Phase 3

Detailed Description

Inflammation is a key to atherosclerosis and its serious effects (MI, stroke) and represents a potentially transformative therapeutic target. Several recent trials have revealed potential outcome benefit in patients with cardiovascular disease who are treated with anti-inflammatory therapy. The LoDoCo trial showed a reduction in cardiovascular events in patients with known coronary artery disease who were treated with colchicine (1). Although the LoDoCo trial did show outcome benefit, and in fact reduced the number of acute coronary syndromes in the treatment group, colchicine's mechanism of action at the level of the atherosclerotic plaque in patients remains unknown. The COLCOT trial was recently published which further demonstrated the potential benefit of anti-inflammatory therapy with colchicine. Compared with placebo, colchicine significantly reduced the risk of cardiovascular events for patients who recently experienced a myocardial infarction (2). It is not clear at the present time whether colchicine mainly reduces the systemic inflammatory milieu, thus reducing the likelihood of plaque progression and new lesion formation; or whether it has a direct effect on local "hot" inflammatory cell activity, thus pacifying the inflammatory plaque to reduce events. Insight into colchicine's mechanism of action may help identify the most suitable patients for this novel approach.

Inflammation imaging may represent a solution. 18F-fluorodeoxyglucose (FDG) PET-CT imaging can define plaque inflammation and identify patients and plaques vulnerable to rupture and events. Improved FDG uptake occurs with therapies that effectively reduced vascular events but not with therapies with no outcome benefit. It is provocative to consider inflammation imaging strategies that may positively impact therapy decisions to yield better patient selection and outcomes.

The long term goal of this research is to determine the pathobiological effect of colchicine on plaque inflammation and determine if inflammation imaging can be integrated into clinical assessment to personalize decisions for anti-inflammation therapies. As the first step for this goal, the investigators propose The CADENCE study is the first step towards this goal; it is a mechanistic and proof-of-concept study that will be the basis for a future 2nd phase definitive RCT integrating inflammation imaging and biomarker strategies.

The Primary Objective is to determine colchicine's effect on plaque inflammation in the carotids and thoracic aorta using FDG PET in patients with diabetes (T2DM) or pre-diabetes and recent vascular events (ACS/MI, TIA, stroke).

Secondary Objectives include defining if baseline inflammation imaging or biomarkers predict the inflammation response.

Exploratory Objectives will define differences in response to colchicine: i) in patients with coronary versus cerebral events; ii) in different vascular beds; iii) relationships of inflammation imaging to other biomarkers.

Methods:

In CADENCE patients with T2DM or pre-diabetes and a recent NonSTEMI, STEMI, stroke or TIA will be recruited to one of 2 arms: colchicine 0.6 mg daily; or placebo. At baseline and 6 months: patients will have FDG PET-CT of carotids and aorta; at 0, 3 and 6 months: clinical evaluation and blood sampling for inflammation biomarkers (eg hsCRP, IL-6, IL-1β, TNF-α, MCP-1). The primary endpoint will be the change in inflammation over 6 months measured as FDG tissue to blood ratio (TBR).

Expected Outcomes:

It is expected that colchicine will reduce arterial inflammation measured using FDG PET/CT and reduce biomarkers of systemic inflammation. This would validate that colchicine's mechanism of action is more than a systemic effect but also a local action at the level of the vessel wall and plaque. This would further identify arterial inflammation on imaging as a potential novel therapeutic target for those at high risk of CV events. Such proof-of-concept data would support a definitive RCT to evaluate and define the potential for new biomarker-imaging strategies to direct decisions for novel anti-inflammation therapies in this population.

Current therapies focus on lesion stenosis or ischemia with conflicting results. Use of inflammation imaging to direct proven treatments would be a bold practice-changing paradigm shift.

Study Design

Study Type:

Interventional

Anticipated Enrollment :

115 participants

Allocation:

Randomized

Intervention Model:

Parallel Assignment

Masking:

Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)

Masking Description:

Double-Blind Placebo controlled trial

Primary Purpose:

Treatment

Official Title:

The Canadian Study of Arterial Inflammation in Patients With Diabetes and Recent Vascular Events: EvaluatioN of Colchicine Effectiveness (CADENCE)

Actual Study Start Date :

Aug 1, 2020

Anticipated Primary Completion Date :

Jun 30, 2023

Anticipated Study Completion Date :

Dec 31, 2023

Arms and Interventions

Arm	Intervention/Treatment
Placebo Comparator: Placebo Placebo: Sugar pill manufactured to mimic colchicine 0.6 mg capsule. Placebo to be taken once a day.	Drug: Placebo oral capsule Patents will be randomized to receive either placebo or colchicine
Experimental: Colchicine Colchicine: 0.6 mg colchicine capsule to be taken once a day.	Drug: Colchicine Oral Product Patients will be randomized to receive either placebo or colchicine

Arm

Intervention/Treatment

Placebo Comparator: Placebo

Placebo: Sugar pill manufactured to mimic colchicine 0.6 mg capsule. Placebo to be taken once a day.

Drug: Placebo oral capsule

Patents will be randomized to receive either placebo or colchicine

Experimental: Colchicine

Colchicine: 0.6 mg colchicine capsule to be taken once a day.

Drug: Colchicine Oral Product

Patients will be randomized to receive either placebo or colchicine

Outcome Measures

Primary Outcome Measures

6 month change in FDG uptake TBR (Tissue to Blood Ratio) in the MDS (Maximum Disease Segment) [6 months]
The primary endpoint will be the change over 6 months in the FDG uptake TBR (Tissue-to-blood ratio) as a marker of arterial plaque inflammation in the maximum disease segment (MDS)(the segment with the highest TBR at baseline) in any vasculature imaged whether it be left or right carotid or aorta.

Secondary Outcome Measures

6 month change in FDG uptake TBR (Tissue to Blood Ratio) in the MDS of each vascular region: aorta, left and right carotid. [6 months]
6 month change in FDG uptake TBR (Tissue to Blood Ratio) in the MDS of each vascular region: aorta, left and right carotid.
6 month change in FDG uptake SUV (standard uptake value) in the MDS of each vascular region: aorta, left and right carotid. [6 months]
6 month change in FDG uptake SUV (standard uptake value) in the MDS of each vascular region: aorta, left and right carotid.
Levels of high-sensitivity C-Reactive Protein (hs-CRP) (mg/ml) and its change [6 months]
Levels of high-sensitivity C-Reactive Protein (hs-CRP) (mg/ml) and its change
Levels of Interleukin-6 (IL-6) (pg/ml) and its change. [6 months]
Levels of Interleukin-6 (IL-6) (pg/ml) and its change.

Other Outcome Measures

Exploratory outcomes - Plasma levels of cytokines (pg/ml) [6 months]
Plasma levels of other cytokines (pg/ml)
Exploratory outcomes - Levels of activated monocytes [6 months]
Levels of activated monocytes
Exploratory outcomes - plasma levels of inflammation biomarkers [6 months]
Plasma levels of inflammation biomarkers
Exploratory outcomes - MACE [6 months]
MACE (multiple adverse CV events (ACS/MI, TIA, stroke, CV death))
Exploratory outcomes - non CV death [6 months]
non-cardiovascular death

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years and Older

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria:

Patients who have:

Type 2 Diabetes (on diet, oral hypo-glycemic agents and/or insulin) or pre-diabetes (defined by Diabetes Canada as HbA1C=6.0-6.45% or increased fasting blood sugar (FBS) (6.1-6.9 mmol/L) or impaired glucose tolerance);
suffered a recent cardiovascular event (≤120 days post ACS (i.e. STEMI or nonSTEMI) or TIA/stroke with ipsilateral large vessel atherosclerotic disease confirmed on US, CT or MRI;
stable symptoms and hemodynamics;
age ≥18 years;
given informed consent. Standard definitions will be used for STEMI, NSTEMI, and for ischemic stroke confirmed by CT or MRI and TIA confirmed by a neurologist.

Exclusion Criteria:

Patients who have

planned revascularization of infarct or stroke related artery more than 120 days after the qualifying/index event;
a recent CV event likely to have been embolic in the opinion of the neurologist or cardiologist;
severe LV dysfunction (EF<30%);
severe valve disease requiring intervention;
decompensated heart failure;
active infection (e.g. pneumonia, active skin infections, and on antibiotics);
chronic diarrhea;
immune compromise (e.g. recurrent infection);
history of cancer within the last 3 years (other than a successfully treated cutaneous squamous cell or basal cell carcinoma or localized carcinoma in situ of the cervix).
active inflammatory conditions (e.g. rheumatoid arthritis, chronic inflammatory bowel disease, SLE, systemic anti-inflammatory therapy (e.g. prednisone, methotrexate));
pregnancy (all women of child bearing potential will have a negative BHCG test;
breastfeeding;
Women of childbearing potential who refuse to use two forms of contraception (this includes at least one form of highly effective and one effective method of contraception) throughout the study OR men capable of fathering a child who refuse to use contraception.
glomerular filtration rate (GFR) <50 ml/min/1.72m2
Use of potent p-glycoprotein inhibitors (i.e. systemic cyclosporine, clarithromycin, or systemic ketoconazole) or a strong CYP3A4 inhibitor (i.e. ritonavir, clarithromycin, or systemic ketoconazole);
Hemoglobin < 105(women) <110 (men) g/L; WBC < 3.0x 10(9)/L, platelet count< 110x 10(9)/L;
Patient with a history of cirrhosis, chronic active hepatitis or severe hepatic disease or with alanine aminotransferase (ALT) levels greater than 3 times the upper limit of normal.
unable to give informed consent;