3X: Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities

• Sponsor: University of Michigan (Other)
• Overall Status: Recruiting
• CT.gov ID: NCT05365334
• Collaborator: (none)
• Enrollment: 45
• Location: 1
• Arms: 3
• Anticipated Duration (Months): 59.6
• Patients Per Site Per Month: 0.8

Study Details

Study Description

Brief Summary

The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.

Condition or Disease	Intervention/Treatment	Phase
Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities; Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile; Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities	Behavioral: Low intensity exercise session; Behavioral: Moderate intensity exercise session; Behavioral: High intensity exercise session	N/A

Study Design

• Study Type: Interventional
• Anticipated Enrollment: 45 participants
• Allocation: Randomized
• Intervention Model: Parallel Assignment
• Masking: None (Open Label)
• Primary Purpose: Basic Science
• Official Title: Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities
• Actual Study Start Date: Dec 13, 2021
• Anticipated Primary Completion Date: Dec 1, 2026
• Anticipated Study Completion Date: Dec 1, 2026

Arms and Interventions

Arm	Intervention/Treatment
Experimental: low-intensity continuous exercise subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes	Behavioral: Low intensity exercise session; subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes
Experimental: moderate-intensity continuous exercise subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes	Behavioral: Moderate intensity exercise session; subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes
Experimental: high-intensity interval exercise subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between	Behavioral: High intensity exercise session; subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between

Outcome Measures

Primary Outcome Measures

1. post-translational modifications of skeletal muscle oxidative enzymes [30 minutes before exercise]

   Western blot

2. post-translational modifications of skeletal muscle oxidative enzymes [immediately after exercise]

   Western blot

3. post-translational modifications of skeletal muscle oxidative enzymes [60 minutes after exercise]

   Western blot

4. post-translational modifications of Adipose tissue metabolic proteins [15 minutes before exercise]

   Western blot

5. post-translational modifications of Adipose tissue metabolic proteins [75 minutes after exercise]

   Western blot

6. mRNA expression of skeletal muscle oxidative enzymes [30 minutes before exercise]

   qPCR

7. mRNA expression of skeletal muscle oxidative enzymes [immediately after exercise]

   qPCR

8. mRNA expression of skeletal muscle oxidative enzymes [60 minutes after exercise]

   qPCR

9. mRNA expression of adipose tissue metabolic enzymes [15 minutes before exercise]

   qPCR

10. mRNA expression of adipose tissue metabolic enzymes [75 minutes after exercise]

    qPCR

Eligibility Criteria

Criteria

• Ages Eligible for Study: 18 Years to 40 Years
• Sexes Eligible for Study: All
• Accepts Healthy Volunteers: Yes

Inclusion Criteria:

• Age:18-40

• Body Mass Index: 20-30 kg/m2

• Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session

• Women must have regularly occurring menses and must be premenopausal

• Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository

Exclusion Criteria:

• Evidence/history of cardiovascular or metabolic disease.

• Taking anti-coagulate medications or medications known to affect metabolism or inflammation

• Weight instability ≥ ±3kg in the last 6 months

• Tobacco or e-cigarette users

• Women must not be pregnant or actively lactating

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	University of Michigan	Ann Arbor	Michigan	United States	48109

Sponsors and Collaborators

• University of Michigan

Investigators

None specified.

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

• Responsible Party: Jeffrey F Horowitz, Professor of Movement Science and Director of the Substrate Metabolism Laboratory (SML) at the University of Michigan School of Kinesiology, University of Michigan
• ClinicalTrials.gov Identifier: NCT05365334
• Other Study ID Numbers: HUM00204857
• First Posted: May 9, 2022
• Last Update Posted: May 12, 2022
• Last Verified: May 1, 2022

• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No

Study Results

No Results Posted as of May 12, 2022