Cellular Stress Reactions During Graft-versus-host Disease
Study Details
Study Description
Brief Summary
This study has the aim to analyze intestinal expression of cellular stress molecules in patients with intestinal GVHD. Patients with colitis and patients without intestinal inflammation will serve as controls.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
Acute graft-versus-host disease (GVHD) is a major cause of morbidity and mortality in patients undergoing allogeneic hematopoietic cell transplantation (allo-HCT). Acute GVHD results from a complex multi-step crosstalk between extensive epithelial tissue damage in the patient and activation of the allo-reactive immune system transferred with the donor graft. The conditioning treatment prior to allo-HCT creates an inflamed microenvironment with high concentrations of pathogen-associated and danger-associated molecular patterns (PAMPs and DAMPs) as well as pro-inflammatory cytokines. This inflammation is perpetuated by activation of myeloid immune cells and recruitment of allo-reactive T cells to the intestine. Enterocytes are subjected to cellular stress and undergo apoptosis. As a result, patients can develop high-voluminous diarrhea, dehydration, intestinal bleeding, hypalbuminemia, and generalized infections.
In this study, the investigators aim to analyze the expression of molecules related to different types of cellular stress in the intestine of patients with acute GVHD. The hypothesis is that some stress-related markers would be upregulated during GVHD. The results will be valuable to study the role of cellular stress reactions during acute GVHD pathogenesis or as potential biomarkers for disease activity.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Uninflamed intestine Patients who underwent diagnostic endoscopy and received a diagnosis of no intestinal inflammation |
Diagnostic Test: Immunohistochemistry on intestinal biopsies
Immunohistochemistry will be performed on human intestinal biopsies
|
| Colitis Patients with active colitis |
Diagnostic Test: Immunohistochemistry on intestinal biopsies
Immunohistochemistry will be performed on human intestinal biopsies
|
| Acute GVHD Patients with acute gastrointestinal (GI) GVHD |
Diagnostic Test: Immunohistochemistry on intestinal biopsies
Immunohistochemistry will be performed on human intestinal biopsies
|
Outcome Measures
Primary Outcome Measures
- Immunohistochemical staining [1 year]
Immunohistochemical staining
Eligibility Criteria
Criteria
Inclusion Criteria:
Age >18 years intestinal biopsy depending on cohort: healthy intestinal tissue, colitis or GVHD
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Medical Center University of Freiburg | Freiburg | Baden-Württemberg | Germany | 79106 |
Sponsors and Collaborators
- University of Freiburg
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- Cellular stress in GVHD