Cholesterol Metabolism and Lipid Transfer in Diabetes
Study Details
Study Description
Brief Summary
The diabetic dyslipidemia is one of the most important risk factor in the development of coronary artery disease. The low density lipoprotein (LDL)-like nanoemulsions is being used to study the clearance of cholesteryl ester and free cholesterol from intravascular in patients with advanced coronary artery disease and it was shown a higher removal of free cholesterol and higher deposit in vases of this patients. The aim of this study is to analyze the plasma kinetics of both forms of cholesterol(free ad esterified) in type 2 diabetes patients without a previous history of cardiovascular disease.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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Detailed Description
Hypercholesterolemia is not typical of type 2 diabetes mellitus dyslipidemia, but alterations in LDL may occur related to atherogenesis.Cholesterol exist in two main forms in organism: free cholesterol and the esterified cholesterol. An artificial model to study this two forms of cholesterol is the use of LDL-like nanoemulsion doubly labeled with 14C-cholesteryl esters and 3H- cholesterol. This nanoemulsion is made without protein and when in the intravascular compartment it is able to acquires apolipoproteins from others lipoproteins, such apos A's, C's and E. This model allowed us to use the nanoemulsion particle as a probe to study the lipoproteins receptor binding and the cholesterol esterification process.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| type 2 diabetes patients with diagnosis of Type 2 diabetes mellitus |
Other: Plasma kinetic study
this study is done with the injection of LDL-like nanoemulsion doubly labeled with 14C- cholesteryl oleate and 3H- cholesterol, with a total radioactivity injection dose of 0.03mSV. Blood sample collected in a pre established period of time in 24 hours.
Other Names:
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| Control non type 2 diabetes mellitus |
Other: Plasma kinetic study
this study is done with the injection of LDL-like nanoemulsion doubly labeled with 14C- cholesteryl oleate and 3H- cholesterol, with a total radioactivity injection dose of 0.03mSV. Blood sample collected in a pre established period of time in 24 hours.
Other Names:
|
Outcome Measures
Primary Outcome Measures
- to identify the removal of the free and esterified cholesterol in type 2 diabetes patients [day of test]
Eligibility Criteria
Criteria
Inclusion Criteria:
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without a previous cardiovascular disease
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if arterial hypertension, or in use of antihypertensive drugs- it must be well controled with a systolic Blood pressure level <130mmHg and diastolic <85mmHg.
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total cholesterol < 6 mmol/L
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LDL- cholesterol <4 mmol/L
Exclusion Criteria:
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use of drugs- statins, fibrates, glucocorticoids, thiazolidinediones
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nephropathy- the presence of microalbuminuria(ratio of microalbuminuria/creatinin >30 ug/mg) or serum creatinin above 98umol/L in woman and above 115 umol/L in man
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retinopathy
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neuropathy presence of chronic disease: heart failure, chronic obstructive pulmonary disease, inflammatory disease and cancer
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Endocrinology service and Lipid laboratory of Heart Institute of University of São Paulo | São Paulo | Brazil | 05423000 |
Sponsors and Collaborators
- University of Sao Paulo General Hospital
- Fundação de Amparo à Pesquisa do Estado de São Paulo
Investigators
- Principal Investigator: Antonio C Lerario, MD, pHd, University of São Paulo
Study Documents (Full-Text)
None provided.More Information
Publications
- Couto RD, Dallan LA, Lisboa LA, Mesquita CH, Vinagre CG, Maranhão RC. Deposition of free cholesterol in the blood vessels of patients with coronary artery disease: a possible novel mechanism for atherogenesis. Lipids. 2007 May;42(5):411-8. Epub 2007 Apr 19.
- Santos RD, Chacra AP, Morikawa A, Vinagre CC, Maranhão RC. Plasma kinetics of free and esterified cholesterol in familial hypercholesterolemia: effects of simvastatin. Lipids. 2005 Jul;40(7):737-43.
- Santos RD, Hueb W, Oliveira AA, Ramires JA, Maranhão RC. Plasma kinetics of a cholesterol-rich emulsion in subjects with or without coronary artery disease. J Lipid Res. 2003 Mar;44(3):464-9. Epub 2002 Dec 1.
- LIPIDSDM