DAPA-GUT: Impact of Dapagliflozin on Intestinal Microbiota Composition and on the Metabolites Derived From the Intestinal Microbiota in Non-diabetic Chronic Renal Failure Patients

Sponsor

Hospices Civils de Lyon (Other)

Overall Status

Not yet recruiting

CT.gov ID

NCT05965440

Collaborator

(none)

Enrollment

Location

Arm

15.5

Anticipated Duration (Months)

3.2

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Chronic kidney disease (CKD) is a common disease affecting 10-12% of the adult population and characterize with high-risk cardiovascular morbidity and mortality with progression of CKD. Treatment with sodium-glucose cotransporter 2 inhibitors (iSGLT2) not only improves hyperglycemia and type 2 diabetes (T2D) but also results in body-weight loss, a reduction in blood pressure, and a decrease of cardiovascular events and progression of renal failure in both diabetes and non-diabetes patients.(Heerspink et al. 2020) Therefore, dapagliflozin is now associated with the inhibitors of the renin-angiotensin system to reduce kidney events.

However, the mechanisms underlying the effects of dapagliflozin on the renal function remain unclear. When renal failure occurs, it impairs the removal of several metabolites called uremic retention solutes. If these retention solutes exhibit deleterious interferences with biochemical/physiological functions, they are referred to as uremic toxins as they can contribute to the manifestations of the uremic syndrome and are associated with a high cardiovascular morbidity and mortality and with progression of CKD. Many of the uremic toxins are not produced by the body itself but rather derived from gut microbiota metabolism such as the well-known trimethylamine-N-oxide (TMAO),p-cresyl sulfate (PCS), phenyl sulfate (PS), indoxyl sulfate (IS), and indole-3-acetic acid (IAA).The gut microbiota composition in a uremic context has been the subject of an increasing number of publications and majority of them confirm a decrease of gut microbiota richness and deep modifications.Recently, an animal study suggested that dapagliflozin, subtly improve the composition of the gut microbiota in mice with T2D and another preliminary clinical study didn't observe a modification in the fecal microbiome after dapagliflozin initiation.But in other study, empagliflozin significantly reshaped the gut microbiota after 1 month of treatment in T2D patients and be associated with shifts in plasma metabolites. Similarly, canagliflozin reduces plasma uremic toxins in a CKD mice model.However, it remains unknown whether treatment with dapagliflozin alters the gut microbiota in CKD patients without T2D; furthermore, the relationship between the gut microbiota, uremic toxins production and CKD-related beneficial effects of dapagliflozin remains elusive.

Herein, the investigator will investigate the clinical benefits of dapagliflozin and possible associations between its renal function benefits and alterations in plasmatic gut microbiota-derived metabolites and the gut microbiota composition in non-T2D CKD patients. To this end, the investigator will conduct an observational clinical trial in non-T2D CKD patients with the primary aim of investigating dapagliflozin-induced compositional changes of intestinal gut microbiota.

Condition or Disease	Intervention/Treatment	Phase
Chronic Kidney Diseases	Drug: impact of Dapagliflozin on Intestinal Microbiota on chronic renal failure patients	N/A

Study Design

Study Type:

Interventional

Anticipated Enrollment :

50 participants

Allocation:

N/A

Intervention Model:

Single Group Assignment

Masking:

None (Open Label)

Primary Purpose:

Diagnostic

Official Title:

Impact of Dapagliflozin on Intestinal Microbiota Composition and on the Metabolites Derived From the Intestinal Microbiota in Non-diabetic Chronic Renal Failure Patients

Anticipated Study Start Date :

Sep 1, 2023

Anticipated Primary Completion Date :

Dec 15, 2024

Anticipated Study Completion Date :

Dec 15, 2024

Arms and Interventions

Arm	Intervention/Treatment
Other: impact of Dapagliflozin on Intestinal Microbiota on chronic renal failure patients Addition of 3 blood tubes of 5mL during the collection for the treatment Collection of fresh urine (7 mL) Collection of stools by the participant at his home Collection of fresh stools for patients participating in the ancillary study Constitution of a biocollection (blood, urine and stool) Food collection for 3 days with no impact on patient follow-up Stool appearance sheet to be completed by the patient at each collection	Drug: impact of Dapagliflozin on Intestinal Microbiota on chronic renal failure patients Analysis of microbiota

Arm

Intervention/Treatment

Other: impact of Dapagliflozin on Intestinal Microbiota on chronic renal failure patients

Addition of 3 blood tubes of 5mL during the collection for the treatment Collection of fresh urine (7 mL) Collection of stools by the participant at his home Collection of fresh stools for patients participating in the ancillary study Constitution of a biocollection (blood, urine and stool) Food collection for 3 days with no impact on patient follow-up Stool appearance sheet to be completed by the patient at each collection

Drug: impact of Dapagliflozin on Intestinal Microbiota on chronic renal failure patients

Analysis of microbiota

Outcome Measures

Primary Outcome Measures

Alpha diversity and beta diversity size of the intestinal microbiota after 12 weeks of daily treatment with dapagliflozin compared to alfa and beta diversity size before initiation of treatment. [Before and 12 weeks after initiation of treatment with dapagliflozin]
The analysis of the microbiota by 16s sequencing of bacterial DNA will be carried out on the GenEPII platform located at the Croix Rousse hospital, Hospices Civils de Lyon (Pr Sophie JARRAUD).

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 80 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

Age between 18 and 80 years old
Non-diabetic patient
Patient with a medical indication for the introduction of dapagliflozin according to the Marketing Authorization and as part of routine care, ie:
An estimated GFR between 25 and 60 mL/min/1.73m2 according to the CKD EPI formula.
A urinary albumin/creatinine ratio > 200mg/g and < 5000 mg/g
Be treated with the maximum tolerated dose of renin-angiotensin system inhibitors for at least 4 weeks.
BMI between 18 and 30 kg/m2
Patient not taking dapagliflozin (or any other treatment containing iSGLT2 or iSGLT1)
Very regular bowel movements between 24 and 48 hours
Patient following the dietary recommendations recommended during CKD (a sodium intake targeting 6g NaCl/day +/-20% and a protein intake of 0.6g/kg/d +/-20%)
Affiliation to social security

Exclusion Criteria:

Taking drugs can interfere with the intestinal microbiota (prebiotics, probiotics, postbiotics, antibiotics) in the last 6 weeks
Patient using high dose laxatives (more than 2 per day, for more than 3 months)
Patient with a foreseeable transplant or dialysis project within the next 6 months.
Patient with a colectomy, a resection of the small intestine or a cholecystectomy
Patient with a progressive and unstabilized inflammatory, infectious, cardiovascular or neoplastic disease
Inability to understand the nature, follow-up and possible consequences of the study.
Patient in exclusion period from previous study or already participating in a clinical research protocol having an impact on the endpoints of the study
Patient under guardianship or in safeguard of justice
Pregnant, parturient or breastfeeding women