Impact of Chronic Rhinosinusitis on the Index of Ciliary Beat Efficiency Using Fluorescent Nanosticks: (R-IMPAC)
Study Details
Study Description
Brief Summary
Impact of chronic rhinosinusitis on the index of ciliary beat efficiency using fluorescent nanosticks
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
To date, the assessment of ciliary beat is only possible ex vivo on epithelial cells obtained from upper airway brushing. A previous prospective study (I-IsBac) showed a change in ciliary beat (in terms of coordination and frequency) in bacterial rhinosinusitis. The study of ex-vivo ciliary movement appears to be an interesting tool to understand the pathophysiology of CSRs and to guide and evaluate treatment.
A new tool to evaluate the effectiveness of the ex-vivo lash beat has been developed. This tool measures shear stress by tracking balls along the ciliary margin.
This measurement of ex-vivo shear stress by bead tracking is a validated technique. However, microbead tracking is limited by its low spatial and temporal resolution, long measurement time and heavy post-processing of acquisition data, making this method difficult to use in clinical routine. Monitoring ex-vivo fluorescent nano-batons could represent a simpler alternative for the clinician. This measurement is now made possible by Phosphate Lanthanum Lanthanum Nano-batons (LaPO4) whose luminescence is directly proportional to the shear.
The objective of this research project is to validate this new tool in patients with CSR by comparing it to a group of control subjects free of nasal inflammation.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Other: Patient with Chronic Rhinosinusitis (CRS) Patients with endonasal surgery scheduled under general anesthesia for an indication of Chronic Rhinosinusitis (CRS) |
Procedure: nasal brushing and bacteriological sample
A nasal brushing as well as a bacteriological sample using a swab under general anesthesia at the beginning of the endonasal surgery procedure
|
| Other: Patient without Chronic Rhinosinusitis (CRS) Patients with endonasal surgery scheduled under general anesthesia for an indication of endonasal surgery for nasal obstruction or patients requiring an endonasal surgical approach such as pituitary adenomas for example. |
Procedure: nasal brushing and bacteriological sample
A nasal brushing as well as a bacteriological sample using a swab under general anesthesia at the beginning of the endonasal surgery procedure
|
Outcome Measures
Primary Outcome Measures
- The shear stresss by tracking nano-batons (mPa) [Day of the surgical procedure under General Anesthesia]
The value of the shear stress by tracking nano-batons in mPa
Secondary Outcome Measures
- Mucosal inflammation score (0 to 10) [Inclusion Visit]
Mucosal inflammation score and presence of polyps during endoscopy
- Presence of pus at endoscopy [Inclusion Visit]
Presence of pus at endoscopy
- Frequency of ciliary beat [Day of the surgical procedure under General Anesthesia]
Frequency of the ciliary beat
- Shear stress by Tracking microbeads (mPa) [Day of the surgical procedure under General Anesthesia]
Value of shear stress by tracking microbeads in mPa
- Presence of polyps at endoscopy [Inclusion Visit]
Presence of polyps at endoscopy
- coordination of cillary beat [Day of the surgical procedure under General Anesthesia]
Cillary beat coordination evaluated by microscopy
Eligibility Criteria
Criteria
Inclusion Criteria:
- Patient ≥ 18 years old
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Having chronic rhinosinusitis (RCS) requiring surgical treatment. Or
-
With an indication for endonasal surgery for nasal obstruction (turbinoplasty and septoplasty) or for an endonasal surgical approach
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Acceptance to participate in the protocol with signed informed consent
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Affiliated or beneficiary of a social security scheme
Exclusion Criteria:
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Refusal to participate in the study
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No one who does not speak or understand French
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Pregnant or breastfeeding woman
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Persons who are incapable of reaching the age of majority, under guardianship, guardianship or protection of justice.
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Simulated participation in another intervention research
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Centre Hospitalier Intercommunal de Créteil | Créteil | France | 94000 | |
| 2 | Hôpital Bicêtre | Le Kremlin-Bicêtre | France |
Sponsors and Collaborators
- Centre Hospitalier Intercommunal Creteil
- Foundation for Medical Research
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- R-IMPAC