Study of Circadian Clock Properties in Healthy Subjects, Obese and Type 2 Diabetic Patients.
Study Details
Study Description
Brief Summary
The investigators aimed to characterize human circadian clockwork in healthy individuals in comparison to obese and type 2 diabetic subjects, employing the noninvasive experimental approach allowing the study of circadian profile in human individuals.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
The investigators specifically aim to obtain and analyze the circadian profile of skin fibroblasts from healthy, obese and T2D subjects. Does circadian oscillator profile and its parameters (phase, period and amplitude) will differ between healthy individuals and obese/ T2D patients? Skin biopsy will be performed in order to collect and culture skin fibroblast cells.
In vivo bioluminescence recording will be performed in primary human skin fibroblast cells. The profiles obtained from 10 subjects in each group will be compared to their healthy counterparts.
Bmal1and Per2 are clock genes. Lentiviral transduction of Bmal1-luciferase or Per2-luciferase in fibroblasts allow observation of circadian oscillations using bioluminescence assay. Phase, period length and amplitude are used to characterize the circadian rhythm.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Healthy Healthy volunteers with no inflammation diseases, no neoplasia, no allergy to lidocaine. |
Other: Circadian rhythm measurement
Procedure: Skin biopsy
A 2mm diameter skin biopsy is performed by a physician using standard procedure.
|
| Type 2 diabetic non obese Type 2 diabetic patients with HbA1c>6.5%, body mass index 19-30 kg/m2, no inflammation diseases, no neoplasia, no allergy to lidocaine. |
Other: Circadian rhythm measurement
Procedure: Skin biopsy
A 2mm diameter skin biopsy is performed by a physician using standard procedure.
|
| Type 2 diabetic obese Type 2 diabetic patients with HbA1c>6.5%, body mass index >30 kg/m2, no inflammation diseases, no neoplasia, no allergy to lidocaine. |
Other: Circadian rhythm measurement
Procedure: Skin biopsy
A 2mm diameter skin biopsy is performed by a physician using standard procedure.
|
| obese Obese non diabetic patients with HbA1c<6.5%, body mass index >30 kg/m2, no inflammation diseases, no neoplasia, no allergy to lidocaine. |
Other: Circadian rhythm measurement
Procedure: Skin biopsy
A 2mm diameter skin biopsy is performed by a physician using standard procedure.
|
Outcome Measures
Primary Outcome Measures
- Difference of period length [5 days]
Period length will be calculated from oscillation profiles of Bmal1-luciferase or Per2-luc luciferase.
Secondary Outcome Measures
- Difference of amplitude [5 days]
Amplitude will be calculated from oscillation profiles of Bmal1-luciferase or Per2-luc luciferase.
Eligibility Criteria
Criteria
Inclusion Criteria:
-
age 20-80
-
consent obtained
Exclusion Criteria:
-
neoplasia
-
inflammatory disease
-
allergy to lidocaine
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Geneva University Hospital | Geneva | Switzerland | 1205 |
Sponsors and Collaborators
- University Hospital, Geneva
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
- Brown SA, Fleury-Olela F, Nagoshi E, Hauser C, Juge C, Meier CA, Chicheportiche R, Dayer JM, Albrecht U, Schibler U. The period length of fibroblast circadian gene expression varies widely among human individuals. PLoS Biol. 2005 Oct;3(10):e338. Epub 2005 Sep 27.
- Pagani L, Schmitt K, Meier F, Izakovic J, Roemer K, Viola A, Cajochen C, Wirz-Justice A, Brown SA, Eckert A. Serum factors in older individuals change cellular clock properties. Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7218-23. doi: 10.1073/pnas.1008882108. Epub 2011 Apr 11.
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