Combination Stem Cell (MESENDO) Therapy for Utilization and Rescue of Infarcted Myocardium

Sponsor

TCA Cellular Therapy (Industry)

Overall Status

Completed

CT.gov ID

NCT00548613

Collaborator

(none)

Enrollment

Location

Arms

Duration (Months)

1.2

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The purpose of this research study is to determine if the transplant of a combination of stem cells, obtained from the bone marrow of the same patient, is effective for utilization and rescue of infarcted myocardium. End points will be the assessment of development of mature and stable new blood vessels as well as improvement in cardiac function.

This, Phase I, single center, prospective, non-randomized, open-label study will evaluate the safety and feasibility of use of the proposed combination of autologous stem cells. Potential subjects who fulfill clinical and laboratory entry criteria at screening will undergo a process of bone marrow aspiration for preparation of the two types of bone marrow-derived stem /progenitor cells to use. The two bone marrow-derived cell types will be mixed and implanted to patients approximately 2 weeks after bone marrow aspiration. After transplant, patients will be have a 3 month follow-up to evaluate safety as well as functional heart improvement by analysis of symptoms, myocardial perfusion SPECT, and echocardiography.

Study population will include adult male and female subjects, ages 18-70, presenting with acute myocardial infarction and subjects who have had a recent (within 12 months) myocardial infarction and will undergo coronary artery bypass grafting.

Patients will be divided in two groups:

the first group will enroll patients with acute myocardial infarction whom percutaneous coronary intervention restored myocardial flow after 4 hours or greater of the initiation of symptoms,
the second group will enroll patients who are candidates for coronary artery bypass surgery and had a myocardial infarction in the past 12 months.

Patients will receive the cell mixture by intracoronary or intramyocardial infusion, respectively.

The rationale of this clinical study is based on the observation that most attempts using adult stem cells for myocardial regeneration have utilized a source of bone marrow derived progenitor cells with the potential to generate new blood vessel and thus contribute to the revascularization of the ischemic tissue. This therapy seems to be adequate but not sufficient, since it lacks a source of stem cells capable of differentiating and maturing into cardiac muscle cells, thus contributing to the recovery of local contractility. The proposed combination stem/progenitor cell therapy to be used in this protocol is aimed at contributing cell types capable of regenerating both blood vessels and muscle tissues damaged after MI.

Condition or Disease	Intervention/Treatment	Phase
Coronary Artery Disease Coronary Arteriosclerosis Coronary Atherosclerosis Coronary Disease	Biological: MESENDO Biological: MESENDO	Phase 1

Study Design

Study Type:

Interventional

Anticipated Enrollment :

20 participants

Allocation:

Randomized

Intervention Model:

Single Group Assignment

Masking:

None (Open Label)

Primary Purpose:

Treatment

Official Title:

Phase I Study to Determine the Safety and Feasibility of the Use of a Combination Stem Cell Therapy in Patients With Acute Myocardial Infarction

Study Start Date :

Oct 1, 2007

Actual Primary Completion Date :

Dec 1, 2008

Actual Study Completion Date :

Mar 1, 2009

Arms and Interventions

Arm	Intervention/Treatment
Experimental: A Patients with documented acute myocardial infarction (heart attack) occurring within 4-24 hours after onset of symptoms	Biological: MESENDO Intracoronary transplantation of autologous stem cells via balloon catheter
Experimental: B Candidates for coronary artery bypass grafting that suffered a myocardial infarction (heart attack) within the past 12 months	Biological: MESENDO Intracardiac transplantation of autologous stem cells via direct injection

Arm

Intervention/Treatment

Experimental: A

Patients with documented acute myocardial infarction (heart attack) occurring within 4-24 hours after onset of symptoms

Biological: MESENDO

Intracoronary transplantation of autologous stem cells via balloon catheter

Experimental: B

Candidates for coronary artery bypass grafting that suffered a myocardial infarction (heart attack) within the past 12 months

Biological: MESENDO

Intracardiac transplantation of autologous stem cells via direct injection

Outcome Measures

Primary Outcome Measures

Safety [6 months]

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 70 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria:

ARM: A -

Patients with acute myocardial infarction with ST elevation who underwent percutaneous revascularization between 4 and 24 hours after the initiation of symptoms.

Able to give written informed consent
Age: 18 to 70 years
Gender: Male and Female
Acute myocardial infarction occurring within 4-24 hours after onset of symptoms documented by at least one of the following:
ST Segment elevation greater than 2mm in two or more consecutive leads
New Bundle Branch Block with symptoms consistent of MI
Troponin I greater than 2.0 ng/ml (Normal Range 0 - 1.5 ng/ml)
Totally occluded artery as visualized by angiography

ARM - B

Patients who are candidates for coronary artery bypass grafting surgery according to ACC/AHA guidelines and have had a myocardial infarction in the past 12 months.

Able to give written informed consent
Patients with coronary artery disease who need coronary artery bypass surgery according to ACC/AHA guidelines
Patients with Left Ventricular Ejection Fraction £ 40%.
NYHA symptoms Class II (dyspnea with moderate effort)
Defined region of myocardial dysfunction related to previous myocardial infarction (within the past 12 months) involving the anterior, lateral, posterior or inferior walls by either of the followings: echocardiography, ventriculography, MRI, or SPECT.
Age: 18 to 70 years
Gender: Male and Female

Exclusion Criteria:

ARM - A

Pregnancy
Previous angiogenic therapy or myocardial laser therapy
History of cancer within 5 years
Known sensitivity to gentamycin and/or amphotericin B
Use or expected use of antineoplastic drugs
No informed consent or unable to provide informed consent.
Any illness which, in the Investigator's judgment, will interfere with the patient's ability to comply with the protocol, compromise patient's safety, or interfere with the interpretation of the study results.
Any illness which might affect patient's survival over the study follow-up period
History of skeletal muscle disease, either primary (i.e., myopathy) or secondary (i.e., ischemic) or any underlying myopathy such as myasthenia gravis, muscular dystrophy, etc.
Patients with active infectious disease and/or who are known to have tested positive for HIV, HTLV, HBV-sAg, HCV, CMV and/or syphilis
Cardiogenic shock or severe compromise in left ventricular systolic function defined as ejection fraction lower than 20 %.
History of intolerance to amiodarone.
End stage renal disease
Contraindication for MRI
Any significant laboratory abnormality which will in the investigator's opinion interfere with the patient's ability to comply with the protocol, compromise the patient's safety, or interfere with the interpretation of the study result.
Inability to identify the infarct area intra operatively

ARM - B

Previous angiogenic therapy or myocardial laser therapy
History of cancer within 5 years
Cardiogenic shock or severe compromise in left ventricular systolic function defined as ejection fraction lower than 20 %.
Left Ventricular Ejection Fraction ≥ 40%.
Known sensitivity to gentamycin and/or amphotericin B
Use or expected use of antineoplastic drugs
No informed consent or unable to provide informed consent
Any illness which, in the Investigator's judgment, will interfere with the patient's ability to comply with the protocol, compromise patient safety, or interfere with the interpretation of the study results
Any illness which might affect patient's survival over the study follow-up period
History of skeletal muscle disease, either primary (i.e., myopathy) or secondary (i.e.,ischemic) or any underlying myopathy such as myasthenia gravis, muscular dystrophy, etc.
Patients with active infectious disease and/or who are known to have tested positive for HIV, HTLV, HBV-sAg, HCV, CMV and/or syphilis
Poor candidates for coronary artery bypass surgery
Patients who are in need of emergency bypass surgery
History of prior coronary artery bypass surgery
Patients with severe valvular heart disease
History of intolerance to amiodarone
End stage renal disease
Pregnancy
Contraindication for MRI
Any significant laboratory abnormality which will in the investigator's opinion interfere with the patient's ability to comply with the protocol, compromise the patient's safety, or interfere with the interpretation of the study result.
Inability to identify infarct area intra operatively.