Cryosurvival of Embryos From Dysmorphic Oocytes

Sponsor

V.K.V. American Hospital, Istanbul (Other)

Overall Status

Completed

CT.gov ID

NCT00521443

Collaborator

(none)

Location

Study Details

Study Description

Brief Summary

Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.

Condition or Disease	Intervention/Treatment	Phase
Cryopreservation Blastocyst	Procedure: Cryopreservation, thawing and blastocyst culture

Study Design

Study Type:

Observational

Observational Model:

Defined Population

Time Perspective:

Prospective

Arms and Interventions

Arm	Intervention/Treatment
Normal Embryos derived from morphologically normal MII oocytes	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Irregular Shapes Embryos derived from irregularly shaped oocytes.	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Large PVS Embryos derived from oocytes with large perivitelline space.	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Dark Zona Embryos derived from oocytes with dark zona pellucida	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Dark cytoplasm Embryos derived from oocytes with dark cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Vacuolar cytoplasm Embryos derived from oocytes with vacuolated cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Central granulation Embryos derived from oocytes with centrally granulated cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double extra Embryos derived from oocytes with double extracytoplasmic abnormalities	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double combined Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double cytoplasmic Embryos derived from oocytes with any two cytoplasmic anomalies	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Triple extra Embryos derived from oocytes with triple extracytoplasmic anomaly	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Triple combined Embryos derived from oocytes with triple combined anomalies	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Outcome Measures

Primary Outcome Measures

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 45 Years

Sexes Eligible for Study:

Female

Inclusion Criteria:

Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure.
Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle.

Exclusion Criteria:

Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture.
Embryos obtained from abnormally fertilized zygotes.

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	VKV American Hospital	Istanbul		Turkey	34365

Sponsors and Collaborators

V.K.V. American Hospital, Istanbul

Investigators

Study Director: Basak Balaban, B.Sc., Assisted Reproduction Unit of the VKV American Hospital of Istanbul

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

, ,

ClinicalTrials.gov Identifier:

NCT00521443

Other Study ID Numbers:

AH-05/04

First Posted:

Aug 27, 2007

Last Update Posted:

Aug 27, 2007

Last Verified:

Aug 1, 2007

Study Results

No Results Posted as of Aug 27, 2007