WatUP: The Effects of Hydration on Gut Health and Thinking
Study Details
Study Description
Brief Summary
The central hypothesis is that improving hydration through increased water consumption will change the relative abundance of mucolytic bacteria found in the stool. Therefore the specific aims are 1) to quantify intervention effects on fecal microbiota relative abundance and plasma lipopolysaccharide binding protein, 2) observe the effects of the intervention on bowel frequency and signs/symptoms of gastrointestinal stress, and 3) to investigate relations between executive function and hydration status.
Condition or Disease | Intervention/Treatment | Phase |
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N/A |
Detailed Description
A single arm 3-week hydration intervention will be employed where participants increase their water consumption to 2 (F) or 2.5(M) liters per day which is approximately 70% of the AI for daily water consumption. Pre-test and follow-up measures of fecal microbiota, urinary hydration status, cognitive function, circulating markers, and dietary intake will be assessed at baseline and at 3-week follow up via laboratory visits.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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Experimental: Water Intake 3-week intervention period during which articipants will be asked to increase their daily plain water consumption to at least 2.5 L/d of water for males and 2L/d for females. |
Dietary Supplement: Water Intake
Participants will increase plain water consumption to at least 70% of the daily adequate intake for Americans depending on their sex.
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Outcome Measures
Primary Outcome Measures
- Fecal microbiota relative abundance [3 weeks (baseline vs. follow-up)]
changes in the relative abundance of fecal microbiota
- Plasma lipopolysaccharide (LPS) [3 weeks (baseline vs. follow-up)]
changes in circulating LPS
Secondary Outcome Measures
- Copeptin [3 weeks (baseline vs. follow-up)]
changes in plasma copeptin concentration
- 24hr Urine Osmolality [3 weeks (baseline vs. follow-up)]
changes in osmolality (mOsmol/kg) of urine samples
- Attentional accuracy [3 weeks (baseline vs. follow-up)]
Accuracy (%) on a computerized flanker task
- Attentional Reaction Time [3 weeks (baseline vs. follow-up)]
Reaction time (ms) on a computerized flanker task
- Attentional processing speed [3 weeks (baseline vs. follow-up)]
P3 event related potential latency (ms) using a computerized flanker task
- 24hr Urine Specific Gravity [3 weeks (baseline vs. follow-up)]
changes in specific gravity (USG) of urine samples
Eligibility Criteria
Criteria
Inclusion Criteria:
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19-50 years of age
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18.5-34.49 kg/m2
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24-hour UOsm above 500 mOsm/kg
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No antibiotic use over the past 3 months
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Absence of metabolic diseases and use of diuretics
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Agree to maintain typical diet intake (e.g., dietary fiber) patterns during intervention
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Avoid consuming prebiotic and probiotic supplements during study participation
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Not pregnant
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Agree to follow the study protocol
Exclusion Criteria:
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<19 or >50 years of age
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<18.5 or >34.49 kg/m2
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24-hour UOsm <500 mOsm/kg
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Antibiotic use over the past 3 months
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Metabolic diseases and use of diuretics
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Not agree to maintain typical diet intake (e.g., dietary fiber) patterns for the duration of the
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intervention
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Not agree with avoiding consuming prebiotic and probiotic supplements during study participation
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Pregnant
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Not agree to follow study protocol
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | University of Illinois at Urbana-Champaign | Urbana | Illinois | United States | 61801 |
Sponsors and Collaborators
- University of Illinois at Urbana-Champaign
- Division of Nutritional Sciences, University of Illinois at Urbana-Champaign
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- WatUP