PEEPS: Examining the Effects of Estradiol on Neural and Molecular Response to Reward

Sponsor

University of North Carolina, Chapel Hill (Other)

Overall Status

Recruiting

CT.gov ID

NCT05282277

Collaborator

National Institute of Mental Health (NIMH) (NIH)

103

Enrollment

Location

Arms

Anticipated Duration (Months)

1.7

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

This proposal will examine the effects of estradiol administration on perimenopausal-onset (PO) anhedonia and psychosis symptoms as well as on brain function using simultaneous positron emission tomography and functional magnetic resonance imaging (PET-MR).

Condition or Disease	Intervention/Treatment	Phase
Depression Psychosis Anhedonia	Drug: Transdermal Estradiol Drug: Micronized Progesterone Drug: Matching Placebo Patch Drug: Raclopride C11	Phase 4

Detailed Description

The transition to menopause (the "perimenopause") is characterized by increased risk for new onset of depression and psychosis. Our work and that of others has demonstrated that a prominent symptom of perimenopausal-onset (PO) depression is anhedonia, contributing significantly to distress and functional impairment. Additionally, the incidence of psychosis in women may increase during this period. Declining or low levels of estradiol, particularly in the late perimenopause, may play a role in the pathogenesis of PO anhedonia and PO psychosis via effects on mesolimbic brain reward circuitry and dopamine (DA) neurotransmission. Preclinical evidence has established that estradiol modulates dopamine systems and reward-related behaviors and estradiol withdrawal evokes loss of dopaminergic functions. Whereas estrogen therapy has shown benefits in reducing mood and psychotic symptoms in perimenopausal women, no study has examined the neural mechanisms underlying such effects in a transdiagnostic sample.

This project will examine the effects of estradiol administration on perimenopausal-onset (PO) anhedonia and psychosis using simultaneous positron emission tomography and functional magnetic resonance imaging (PET-MR). Preliminary data presented here demonstrate that anhedonia is associated with decreased striatal DA release to rewards using PET with the D2/D3 DA receptor antagonist [11C]raclopride; anhedonia and psychosis are characterized by altered striatal activation to rewards using fMRI; estradiol impacts neural responses to rewards in PO anhedonia and PO psychosis; and estradiol improves PO anhedonia and PO psychosis. This project proposes to extend these lines of research by using simultaneous PET-MR to investigate the effects of transdermal estradiol, administered as a mechanistic probe, on PO anhedonia and PO psychosis in a transdiagnostic sample of women using a double-blind between-groups placebo-controlled design. This sample will be enriched for anhedonia (i.e., at least mild anhedonia). Although anhedonia and psychosis will be analyzed dimensionally, our recruitment and stratification strategy will ensure that a range of symptom severities (mild-to-moderate or high PO anhedonia; absent-to-mild or moderate PO psychosis) are equally balanced and randomized to each experimental group (estradiol or placebo). Our central hypotheses are that the mesolimbic DA system is impaired in PO anhedonia and psychosis, that estradiol administration will normalize neural responses to rewards (measured by fMRI) and striatal DA functioning (measured by PET), and that the degree of change in striatal functioning will be associated with the degree of change in PO anhedonia and PO psychosis.

Specific Aim 1 (baseline associations between PO anhedonia, PO psychosis, and PET-MR):

Characterize, at baseline, associations between PO anhedonia and PO psychosis symptom severity and reward-related striatal activation measured by fMRI, and tonic and phasic striatal DA activity measured by [11C]raclopride PET.

Specific Aim 2 (estradiol effects on PO anhedonia and PET-MR): Determine the effects of estradiol (vs. placebo) on PO anhedonia and changes in PET-MR metrics related to reward processing.

Specific Aim 3 (estradiol effects on PO psychosis and PET-MR): Determine the effects of estradiol (vs. placebo) on PO psychosis and changes in PET-MR metrics related to reward processing.

This project will improve our understanding of PO anhedonia and psychosis and the mechanisms of action of the effect of estradiol on PO anhedonia and psychosis. This research will provide new mechanistic endpoints to evaluate novel PO anhedonia and psychosis treatments that target the mesolimbic DA system.

Study Design

Study Type:

Interventional

Anticipated Enrollment :

103 participants

Allocation:

Randomized

Intervention Model:

Parallel Assignment

Intervention Model Description:

Parallel group, randomized placebo-controlled studyParallel group, randomized placebo-controlled study

Masking:

Double (Participant, Outcomes Assessor)

Primary Purpose:

Basic Science

Official Title:

Examining the Effects of Estradiol on Neural and Molecular Response to Rewards in Perimenopausal-Onset Anhedonia and Psychosis

Actual Study Start Date :

Apr 1, 2022

Anticipated Primary Completion Date :

Apr 1, 2027

Anticipated Study Completion Date :

Apr 1, 2027

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Perimenopausal women with mild-to-moderate anhedonia + absent-to-mild psychosis, active group Participants will be randomly assigned to take 100 μg/day of transdermal estradiol for 3 weeks followed by 1 week of combined 100 μg/day of transdermal estradiol and 200 mg/day progesterone.	Drug: Transdermal Estradiol Participants will be randomized to receive transdermal estradiol (100μg/day) patch for 3 weeks. Other Names: Climara Drug: Micronized Progesterone Participants will receive an additional week of micronized progesterone (200 mg/day) at the end of the study to precipitate menstruation. Other Names: Prometrium Drug: Raclopride C11 All Participants will receive two PET-MR scans using [11C]raclopride IV as the tracer. The first scan will occur at baseline and the second at post treatment after 3 weeks. Other Names: [C11]Raclopride
Experimental: Perimenopausal women with mild-to-moderate anhedonia + absent-to-mild psychosis, placebo group Participants will be randomly assigned to receive a matching placebo patch for 3 weeks.	Drug: Matching Placebo Patch Participants will be randomized to receive a transdermal estradiol-matching placebo patch for 3 weeks Other Names: Placebo Drug: Raclopride C11 All Participants will receive two PET-MR scans using [11C]raclopride IV as the tracer. The first scan will occur at baseline and the second at post treatment after 3 weeks. Other Names: [C11]Raclopride
Experimental: Perimenopausal women with mild-to-moderate anhedonia + moderate psychosis, active group Participants will be randomly assigned to take 100 μg/day of transdermal estradiol for 3 weeks followed by 1 week of combined 100 μg/day of transdermal estradiol and 200 mg/day progesterone.	Drug: Transdermal Estradiol Participants will be randomized to receive transdermal estradiol (100μg/day) patch for 3 weeks. Other Names: Climara Drug: Micronized Progesterone Participants will receive an additional week of micronized progesterone (200 mg/day) at the end of the study to precipitate menstruation. Other Names: Prometrium Drug: Raclopride C11 All Participants will receive two PET-MR scans using [11C]raclopride IV as the tracer. The first scan will occur at baseline and the second at post treatment after 3 weeks. Other Names: [C11]Raclopride
Experimental: Perimenopausal women with mild-to-moderate anhedonia + moderate psychosis, placebo group Participants will be randomly assigned to receive a matching placebo patch for 3 weeks.	Drug: Matching Placebo Patch Participants will be randomized to receive a transdermal estradiol-matching placebo patch for 3 weeks Other Names: Placebo Drug: Raclopride C11 All Participants will receive two PET-MR scans using [11C]raclopride IV as the tracer. The first scan will occur at baseline and the second at post treatment after 3 weeks. Other Names: [C11]Raclopride
Experimental: Perimenopausal women with high anhedonia + absent-to-mild psychosis, active group Participants will be randomly assigned to take 100 μg/day of transdermal estradiol for 3 weeks followed by 1 week of combined 100 μg/day of transdermal estradiol and 200 mg/day progesterone.	Drug: Transdermal Estradiol Participants will be randomized to receive transdermal estradiol (100μg/day) patch for 3 weeks. Other Names: Climara Drug: Micronized Progesterone Participants will receive an additional week of micronized progesterone (200 mg/day) at the end of the study to precipitate menstruation. Other Names: Prometrium Drug: Raclopride C11 All Participants will receive two PET-MR scans using [11C]raclopride IV as the tracer. The first scan will occur at baseline and the second at post treatment after 3 weeks. Other Names: [C11]Raclopride
Experimental: Perimenopausal women with high anhedonia + absent-to-mild psychosis, placebo group Participants will be randomly assigned to receive a matching placebo patch for 3 weeks.	Drug: Matching Placebo Patch Participants will be randomized to receive a transdermal estradiol-matching placebo patch for 3 weeks Other Names: Placebo Drug: Raclopride C11 All Participants will receive two PET-MR scans using [11C]raclopride IV as the tracer. The first scan will occur at baseline and the second at post treatment after 3 weeks. Other Names: [C11]Raclopride
Experimental: Perimenopausal women with high anhedonia + moderate psychosis, active group Participants will be randomly assigned to take 100 μg/day of transdermal estradiol for 3 weeks followed by 1 week of combined 100 μg/day of transdermal estradiol and 200 mg/day progesterone.	Drug: Transdermal Estradiol Participants will be randomized to receive transdermal estradiol (100μg/day) patch for 3 weeks. Other Names: Climara Drug: Micronized Progesterone Participants will receive an additional week of micronized progesterone (200 mg/day) at the end of the study to precipitate menstruation. Other Names: Prometrium Drug: Raclopride C11 All Participants will receive two PET-MR scans using [11C]raclopride IV as the tracer. The first scan will occur at baseline and the second at post treatment after 3 weeks. Other Names: [C11]Raclopride
Experimental: Perimenopausal women with high anhedonia + moderate psychosis, placebo group Participants will be randomly assigned to receive a matching placebo patch for 3 weeks.	Drug: Matching Placebo Patch Participants will be randomized to receive a transdermal estradiol-matching placebo patch for 3 weeks Other Names: Placebo Drug: Raclopride C11 All Participants will receive two PET-MR scans using [11C]raclopride IV as the tracer. The first scan will occur at baseline and the second at post treatment after 3 weeks. Other Names: [C11]Raclopride

Outcome Measures

Primary Outcome Measures

Changes in Striatal Activation Between Groups during the MID task [Baseline (week 3) to Endpoint (week 7)]
Characterize reward-related striatal activation measured by fMRI using the Monetary Incentive Delay (MID) task to elicit blood-oxygen-level dependent (BOLD) responses. During MID the task, participants respond to "win" trials by pressing a button on a button box in the MRI as quickly as possible when the see a target. Reactivity is measured by examining participant's change in blood-oxygen-level dependent (BOLD) response to win trials versus non-win trials. Reactivity is then compared between the two groups.

Secondary Outcome Measures

Changes in PO Psychosis Symptoms following Estradiol Administration Using the BPRS [Baseline (week 3) to Endpoint (week 7)]
Determine the effects of estradiol (vs. placebo) on PO psychosis symptoms using the Brief Psychiatric Rating Scale (BPRS).The BPRS is an 18-item clinician rated psychosis measure that examines the severity of psychotic symptoms. Individuals with psychotic major depression typically have BPRS positive symptom subscale scores 7-11, and a score of 6 best differentiates psychotic MDD from nonpsychotic MDD.
Changes in PO Anhedonia Following Estradiol Administration Using the SHAPS [Baseline (week 3) to Endpoint (week 7)]
Determine the effects of estradiol (vs. placebo) on PO anhedonia symptoms using the Snaith-Hamilton Pleasure Scale (SHAPS). The SHAPS is a 14-item self-rated anhedonia scale. Items are comprised of statements that participants rate as "strongly disagree" (1), "disagree" (2), "agree" (3), or "strongly agree" (4). The lowest possible score is 14, the highest possible score is 56 (greatest anhedonia)
Change in striatal phasic DA release and background DA tone to rewards measured by [11C]raclopride PET using the Monetary Incentive Delay (MID) task. [Baseline (week 3) to Endpoint (week 7)]
Binding potential changes due to DA release and competition will be determined using a dynamic occupancy model. We will use baseline nondisplaceable binding potential (BPND) and change in BPND following task onset (ΔBPND%) to determine differences between groups in terms of the baseline (tonic) state and the activation (phasic) state.

Eligibility Criteria

Criteria

Ages Eligible for Study:

44 Years to 55 Years

Sexes Eligible for Study:

Female

Accepts Healthy Volunteers:

Inclusion Criteria:

Provision of signed and dated informed consent form
Stated willingness to comply with all study procedures, lifestyle considerations, and availability for the duration of the study
44-55 years old unmedicated perimenopausal women who have ≥ 2 skipped menstrual cycles, amenorrhea ≥ 60 days, corresponding to the late menopause transition (Stages of Reproductive Aging Workshop (STRAW stage -1).
Anhedonia or psychosis symptoms that began during the period of menstrual irregularity.
Clinician's Global Impression Scale-Severity score (CGI-S) > 3 to confirm a clinically impaired sample.
Anhedonia severity inclusion criteria and stratification: All participants will have Snaith-Hamilton Pleasure Scale (SHAPS) scores > 20 consistent with the NIMH Fast-Fail Trial for Mood and Anxiety Disorders, corresponding to clinically impairing anhedonia.
Psychosis severity inclusion criteria and stratification: Participants will be stratified according to scores on the psychotic subscale of the Brief Psychiatric Rating Scale (BPRS)
Willingness to adhere to the estradiol regimen

Exclusion Criteria:

Pregnancy; allergies to any active or inactive ingredients in the Climara® patch or Prometrium®.
BMI < 18 or > 35 kg/m^2
A history of chronic menstrual cycle irregularity, meaning > 1 year without menses
MR contraindications: Metal in the body, dental work other than fillings or gold, tattoos, metal injury, any other implant unless they are 100% plastic.
PET contradictions: participation in >1 research study in the past 12 months that included ionizing radiation exceeding 3 rem to the whole body (e.g., PET, CT). Standard of care imaging is not exclusionary.
The use of psychotropics or hormonal preparations.
History of psychiatric illness during the 2 years before the onset of perimenopause.
History of chronic, recurrent mood or psychotic disorders (i.e., more than one non-reproductive-related mood episode prior to the perimenopausal index episode).
A history of mood episodes requiring hospitalization.
Current mania;
Depressive episode(s) within 2 years of enrollment not associated with the transition to menopause;
A history of suicide attempts within the last year or current active suicidal ideation with intent and plan.
Neurological conditions (e.g., history of seizure or TBI)
Brain stimulation treatment in the past six months.
Endometriosis;
First degree relative with premenopausal breast cancer or breast cancer presenting in both breasts or multiple family members (greater than three relatives) with postmenopausal breast cancer.
Current medication use (i.e., current psychotropics, current anti-hypertensives, current statins, current hormonal preparations, or frequent use of anti-inflammatory agents (> 10 times/month)). Women will be allowed to enroll who take medications without known mood effects (e.g. stable thyroid hormone replacement and occasional (< 5 times/month) use of Ambien)*;
Pregnant, breastfeeding or trying to conceive;
Last menstrual period more than 12 months prior to enrollment;
History of undiagnosed vaginal bleeding;
Undiagnosed enlargement of the ovaries;
Polycystic ovary syndrome;
History of breast or ovarian cancer;
First degree relative with ovarian cancer;
Abnormal finding in a provider breast exam and/or mammogram;
Known carrier of BRCA1 or 2 mutation;
Porphyria;
Malignant melanoma;
Hodgkin's disease;
Recurrent migraine headaches that are preceded by aura;
Gallbladder or pancreatic disease**;
Heart or kidney disease**;
Liver disease;
cerebrovascular disease (stroke);
First degree relative with history of heart attack or stroke;
Current nicotine use;
Self-reported claustrophobia
Peanut allergy
all reported prescription medications will be reviewed and cleared by a study physician prior to a participant's enrollment;
participants will be given the opportunity to describe these conditions in the online screening survey. Reported conditions that are acute in nature and/or benign will be reviewed by a study physician and exclusions will be decided case-by-case. All chronic conditions will be exclusionary. For those where it is deemed that an exclusion does not apply, primary analyses will not be affected, but exploratory analyses will be conducted excluding these individuals

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	University of North Carolina at Chapel Hill	Chapel Hill	North Carolina	United States	27514

Sponsors and Collaborators

University of North Carolina, Chapel Hill
National Institute of Mental Health (NIMH)

Investigators

Principal Investigator: Crystal E Schiller, PhD, UNC School of Medicine - Department of Psychiatry
Principal Investigator: Gabriel Dichter, PhD, UNC School of Medicine - CIDD