Discovery of Biological Signatures for Cruciferous Vegetable Intake (Single Serving)
Study Details
Study Description
Brief Summary
The purpose of this project is to study the molecular and metabolic profiles in human plasma, urine and microbiome composition following the consumption of broccoli sprouts.
Condition or Disease | Intervention/Treatment | Phase |
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N/A |
Detailed Description
The investigators wish to examine metabolic and molecular markers found in blood and in urine after a single dose of sprouts, and evaluate the importance of the gut microbiome on these parameters. Some subjects will receive broccoli sprouts that are grown in deuterium oxide, a commonly used non-toxic, non-radioactive label for vegetables (Tang, Qin et al. 2005), so researchers can identify metabolites in blood and urine that are directly from consumed sprouts. The investigators plan to feed subjects a standardized breakfast and either broccoli sprouts or alfalfa sprouts (control) and look at the presence of metabolites and activity in the blood and urine 3, 6, 24, 48 and 72 hours following intake as well as changes in microbiome composition.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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Active Comparator: Broccoli sprouts Subjects will consume one serving (about 1 cup) of broccoli sprouts with breakfast (bagel, cream cheese, and orange juice). |
Other: Broccoli sprouts
Subjects will consume one serving (about 1 cup) of broccoli sprouts with breakfast (bagel, cream cheese, and orange juice).
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Active Comparator: Deuterium oxide-labeled broccoli sprouts Subjects will consume one serving (about 1 cup) of deuterium oxide-labeled broccoli sprouts with breakfast (bagel, cream cheese, and orange juice). |
Other: Deuterium oxide-labelled broccoli sprouts
Subjects will consume one serving (about 1 cup) of deuterium oxide-labelled broccoli sprouts with breakfast (bagel, cream cheese, and orange juice).
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Placebo Comparator: Alfalfa sprouts Subjects will consume one serving (about 1 cup) of alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice). |
Other: Alfalfa sprouts
Subjects will consume one serving (about 1 cup) of alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice).
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Placebo Comparator: Deuterium oxide-labeled alfalfa sprouts Subjects will consume one serving (about 1 cup) of deuterium oxide-labeled alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice). |
Other: Deuterium oxide-labelled alfalfa sprouts
Subjects will consume one serving (about 1 cup) of deuterium oxide-labelled alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice).
|
Outcome Measures
Primary Outcome Measures
- Urine sulforaphane [Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts]
Change in levels of sulforaphane metabolites in urine
- Urine sulforaphane metabolites [Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts]
Change in levels of sulforaphane metabolites in urine
- Plasma sulforaphane [Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts]
Change in levels of sulforaphane in plasma
- Plasma sulforaphane metabolites [Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts]
Change in levels of sulforaphane metabolites in plasma
- Stool sample based gut microbiome composition using 16S rRNA gene sequencing [Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts]
Change in stool sample based gut microbiome composition using 16S rRNA gene sequencing
Eligibility Criteria
Criteria
Inclusion Criteria:
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Healthy men and women, 18-60 years of age
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Willing to stop taking herbal and phytochemical (plant-based extract or phytochemical) supplements for 1 week prior to and during the study
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Willing to stop cruciferous vegetable and probiotic supplement, food and beverage intake 1 week prior to and during the study
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Willing to complete a 10-day food diary during the study
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Must be able to give written informed consent
Exclusion Criteria:
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Body Mass Index (BMI) <18.5 or >30.0 kg/m2
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Tobacco use, including e-cigarettes, or smoking of any substance (e.g. cannabis) in the past three months
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Pregnancy, breastfeeding, or planning to become pregnant before completing the study
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Engaging in vigorous exercise more than 7 hours per week
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Have a significant acute or chronic illness such as cardiovascular disease, kidney or liver disease, diabetes, thyroid disorder, or radiation or chemotherapy treatment for cancer within the past five years.
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Use of medications to control cholesterol (e.g. statins, cholestyramine) or fat absorption (e.g. orlistat)
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Have had bariatric surgery (e.g. gastric bypass, gastric banding, sleeve gastrectomy, etc.), other gastrointestinal procedure (e.g. cholecystectomy) or disorders (e.g. Crohn's disease, celiac disease, ulcerative colitis)
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Use of oral antibiotic medication within the 6 months
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | Oregon State University | Corvallis | Oregon | United States | 97331 |
Sponsors and Collaborators
- Oregon State University
- United States Department of Agriculture (USDA)
Investigators
- Principal Investigator: Emily Ho, PhD, Oregon State University
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- LPI-8343
- 2020-67001-31214