DNA Analysis of Tissue From Patients With T-Cell Acute Lymphoblastic Leukemia

Sponsor

ECOG-ACRIN Cancer Research Group (Other)

Overall Status

Completed

CT.gov ID

NCT00899366

Collaborator

National Cancer Institute (NCI) (NIH)

Enrollment

Actual Duration (Months)

Study Details

Study Description

Brief Summary

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This laboratory study is looking at tissue samples from patients with T-cell acute lymphoblastic leukemia.

Condition or Disease	Intervention/Treatment	Phase
Leukemia	Genetic: microarray analysis Genetic: mutation analysis Other: immunologic technique

Detailed Description

OBJECTIVES:

Identify the transcriptional signatures associated with the presence of NOTCH1 mutations in primary T-cell acute lymphoblastic leukemia (T-ALL) cells using oligonucleotide microarrays.
Characterize the global changes on gene expression resulting from the inactivation of NOTCH signaling in human T-ALL lymphoblasts.

OUTLINE: This is a multicenter study.

Frozen lymphoblast samples from patients with T-cell acute lymphoblastic leukemia (NOTCH1-mutated and wild type) are assessed for genetic expression profiles and mutations by microarray analysis and activated NOTCH1 protein by western blot analysis.

PROJECTED ACCRUAL: A total of 48 samples from patients will be accrued for this study.

Study Design

Study Type:

Observational

Actual Enrollment :

49 participants

Observational Model:

Other

Time Perspective:

Retrospective

Official Title:

Molecular Mechanisms of NOTCH Induced Transformation in T-ALL

Actual Study Start Date :

Dec 28, 2006

Actual Primary Completion Date :

Jul 30, 2009

Actual Study Completion Date :

Jul 30, 2009

Outcome Measures

Primary Outcome Measures

Transcriptional signatures associated with NOTCH1 mutations in T-cell acute lymphoblastic leukemia (T-ALL) cells [1 month]
Mutational analysis
Global changes on gene expression resulting from inactivation of NOTCH signaling in T-ALL cells [1 month]
Gene expression