The Effect of a Diabetic- and a Kidney-like Environment on Gene and Protein Expression in Cultured EC and SMC
Sponsor
Meir Medical Center (Other)
Overall Status
Recruiting
CT.gov ID
NCT01423279
Collaborator
(none)
100
1
113.9
0.9
Study Details
Study Description
Brief Summary
This research is NOT part of an interventional trial, however the investigators use umbilical cord after birth for isolating endothelial and smooth muscle cells for academic research.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
|
Detailed Description
Isolation and culturing of endothelial and smooth muscle muscles for academic research
Study Design
Study Type:
Observational
Anticipated Enrollment
:
100 participants
Observational Model:
Other
Time Perspective:
Other
Official Title:
The Effect of a Diabetic- and a Kidney-like Environment on Gene and Protein Expression in Mono- and Co-cultured Endothelial and Smooth Muscle Cells
Study Start Date
:
Feb 1, 2013
Anticipated Primary Completion Date
:
Aug 1, 2022
Anticipated Study Completion Date
:
Aug 1, 2022
Outcome Measures
Primary Outcome Measures
- The effect of a Diabetic- and a Kidney-like environment on gene and protein expression in mono- and co-cultured endothelial and smooth muscle cells [one year]
Eligibility Criteria
Criteria
Ages Eligible for Study:
N/A
and Older
Sexes Eligible for Study:
Female
Accepts Healthy Volunteers:
Yes
Inclusion Criteria:
- normal pregnancy
Exclusion Criteria:
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Laboratory of Renal Physiology, Meir Medical Center , | Kfar Saba | Israel |
Sponsors and Collaborators
- Meir Medical Center
Investigators
- Study Director: Sydney Benchetrit, MD, Nephrology dept. Meir Medical Center, Kfar Saba , Israel
Study Documents (Full-Text)
None provided.More Information
Publications
- Rashid G, Plotkin E, Klein O, Green J, Bernheim J, Benchetrit S. Parathyroid hormone decreases endothelial osteoprotegerin secretion: role of protein kinase A and C. Am J Physiol Renal Physiol. 2009 Jan;296(1):F60-6. doi: 10.1152/ajprenal.00622.2007. Epub 2008 Oct 22.
- Talmor-Barkan Y, Bernheim J, Green J, Benchetrit S, Rashid G. Calcitriol counteracts endothelial cell pro-inflammatory processes in a chronic kidney disease-like environment. J Steroid Biochem Mol Biol. 2011 Mar;124(1-2):19-24. doi: 10.1016/j.jsbmb.2011.01.001. Epub 2011 Jan 12.
- Talmor-Barkan Y, Rashid G, Weintal I, Green J, Bernheim J, Benchetrit S. Low extracellular Ca2+: a mediator of endothelial inflammation. Nephrol Dial Transplant. 2009 Nov;24(11):3306-12. doi: 10.1093/ndt/gfp354. Epub 2009 Jul 18.
- Zitman-Gal T, Green J, Pasmanik-Chor M, Oron-Karni V, Bernheim J. Endothelial pro-atherosclerotic response to extracellular diabetic-like environment: possible role of thioredoxin-interacting protein. Nephrol Dial Transplant. 2010 Jul;25(7):2141-9. doi: 10.1093/ndt/gfp768. Epub 2010 Jan 19.
Responsible Party:
Meir Medical Center
ClinicalTrials.gov Identifier:
NCT01423279
Other Study ID Numbers:
- MMC11-0074CTIL
- 0074-11-MMC
First Posted:
Aug 25, 2011
Last Update Posted:
Sep 1, 2021
Last Verified:
Aug 1, 2021