Effect of Smoking on Saliva Composition and the Development of Dental Erosion
Study Details
Study Description
Brief Summary
The aim of this study is to investigate whether smoking is associated with changes in salivary composition and/or predisposition to erosion.
Healthy volunteers are observationally wearing an intraoral device with both bovine tooth specimens (enamel and dentin) and resin specimens twice for two hours each. Afterwards, specimens are eroded extraorally and calcium release into the acid is measured.
Total protein concentration and protein composition of the salivary pellicles on the resin samples are measured. Additionally, salivary parameters (unstimulated and stimulated saliva flow rate, pH, buffer capacity, and total protein content as well as concentration of inorganic calcium, phosphate, and fluoride) are measured.
Condition or Disease | Intervention/Treatment | Phase |
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|
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
---|---|
Smokers Volunteers smoking at least 10 cigarettes per day |
Other: Use of fluoridated toothpaste
Use of fluoridated toothpaste
Other: Wearing of an intraoral device with bovine tooth samples
Wearing of an intraoral device with bovine tooth samples
Other: Wearing of an intraoral device with resin samples
Wearing of an intraoral device with resin samples
|
Non-smokers Non-smoking volunteers |
Other: Use of fluoridated toothpaste
Use of fluoridated toothpaste
Other: Wearing of an intraoral device with bovine tooth samples
Wearing of an intraoral device with bovine tooth samples
Other: Wearing of an intraoral device with resin samples
Wearing of an intraoral device with resin samples
|
Outcome Measures
Primary Outcome Measures
- Calcium release from bovine enamel and dentin specimens by extraoral erosion (nmol/Square Millimeter). [Immediately after the intraoral device has been worn once for two hours.]
Secondary Outcome Measures
- Determination of unstimulated and stimulated saliva flow rate (mL/min). [Saliva samples are collected for 5 min each on three visits.]
- Determination of saliva pH (pH). [Saliva samples are collected for 5 min each on three visits.]
- Determination of salivary buffer capacity (pH). [Saliva samples are collected for 5 min each on three visits.]
- Determination of total protein content (mg/L) in saliva. [Saliva samples are collected for 5 min each on three visits.]
- Determination of inorganic calcium (mmol/l), phosphate (mmol/L) and fluoride (µmol/L) in saliva. [Saliva samples are collected for 5 min each on three visits.]
- Determination of total protein concentration (ng/Square Millimeter) and protein composition (qualitatively) within the salivary pellicles. [Immediately after the intraoral device with resin samples has been worn once for two hours.]
Eligibility Criteria
Criteria
Inclusion Criteria:
- Volunteers aged between 20 and 50 years who are able to give written consent
Exclusion Criteria:
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Non-fulfillment of the inclusion criteria
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Intake of medications or diseases altering salivary secretion
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Refusal to use fluoridated toothpastes
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Known allergies to substances used in the study
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Orthodontic treatment or malfunction which does not allow wearing an intraoral device
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | University Medical Center Göttingen, Dept. of Preventive Dentistry, Periodontology and Cariology | Göttingen | Lower Saxony | Germany | 37075 |
Sponsors and Collaborators
- University of Göttingen
Investigators
- Principal Investigator: Annette Wiegand, Prof. Dr. med. dent., Dept. of Prev. Dentistry, Periodontology and Cariology, University Medical Center Göttingen, Germany
Study Documents (Full-Text)
None provided.More Information
Publications
- Carpenter G, Cotroneo E, Moazzez R, Rojas-Serrano M, Donaldson N, Austin R, Zaidel L, Bartlett D, Proctor G. Composition of enamel pellicle from dental erosion patients. Caries Res. 2014;48(5):361-7. doi: 10.1159/000356973. Epub 2014 Mar 6.
- Kanzow P, Wegehaupt FJ, Attin T, Wiegand A. Etiology and pathogenesis of dental erosion. Quintessence Int. 2016 Apr;47(4):275-8. doi: 10.3290/j.qi.a35625. Review.
- Moazzez R, Bartlett D. Intrinsic causes of erosion. Monogr Oral Sci. 2014;25:180-96. doi: 10.1159/000360369. Epub 2014 Jun 26. Review.
- Wiegand A, Bliggenstorfer S, Magalhaes AC, Sener B, Attin T. Impact of the in situ formed salivary pellicle on enamel and dentine erosion induced by different acids. Acta Odontol Scand. 2008 Aug;66(4):225-30. doi: 10.1080/00016350802183401.
- Wiegand A, Meier W, Sutter E, Magalhães AC, Becker K, Roos M, Attin T. Protective effect of different tetrafluorides on erosion of pellicle-free and pellicle-covered enamel and dentine. Caries Res. 2008;42(4):247-54. doi: 10.1159/000135669. Epub 2008 Jun 4.
- Wiegand A, Rosemann A, Hoch M, Barke S, Dakna M, Kanzow P. Erosion-Protective Capacity of the Salivary Pellicle of Female and Male Subjects Is Not Different. Caries Res. 2019;53(6):636-642. doi: 10.1159/000500046. Epub 2019 Jun 4.
- Zwier N, Huysmans MC, Jager DH, Ruben J, Bronkhorst EM, Truin GJ. Saliva parameters and erosive wear in adolescents. Caries Res. 2013;47(6):548-52. doi: 10.1159/000350361. Epub 2013 Jun 15.
- Erosion-Smoking
- 15/10/19
- 02785