Effect of Sperm Selection Techniques on Human Neonatal Gender Ratio in Patients Undergoing ICSI
Study Details
Study Description
Brief Summary
babies of known gender born out of 388 ICSI cycles were investigated for the gender ratio and then divided into three groups according to the sperm selection technique used before performing sperm injection. Statistical analysis were made to compare ratios and compare results of three arms
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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Detailed Description
A total of 529 babies of known gender born out of 388 ICSI cycles between August 2016 and May 2018 at Ganin Fertility Center, Cairo, Egypt, were investigated for the gender ratio and then divided into three groups according to the sperm selection technique used before performing sperm injection; DGC (237 neonates out of 173 ICSI cycles), PICSI (147 neonates out of 109 ICSI cycles) , and MACS (145 neonates out of 106 ICSI cycles). In PICSI and MACS groups. Power analysis was done by comparing the sex ratio of the neonates between DGC, PICSI and MACS. The chi-squared test for independent samples was chosen to perform the power analysis with α-error level at 0.05. P values less than 0.05 were considered statistically significant. All statistical calculations were done using IBM SPSS (Statistical Package for the Social Science; IBM Corp, Armonk, NY, USA) release 22 for Microsoft Windows.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Density Gradient Centrifugation (DGC) semen was layered over 50 % and 90% discontinuous Density Gradient layers in a 15ml conical tube, then centrifuged at 250 g for 8 min at room temperature. supernatant was aspirated and the resulted pellet was washed using Sperm wash media and centrifuged at 250 g for 8 min at room temperature. The final pellet was re suspended in residual volume |
Other: Density Gradient Centrifugation
semen was layered over 50 % and 90% discontinuous Density Gradient layers in a 15ml conical tube, then centrifuged at 250 g for 8 min at room temperature. supernatant was aspirated and the resulted pellet was washed using Sperm wash media and centrifuged at 250 g for 8 min at room temperature. The final pellet was re suspended in residual volume
Other Names:
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| Physiological ICSI (PICSI) Semen processing is done by double layer DGC method followed by adding Sperm to the dot of hyaluronan on the PICSI dish, within minutes the bound sperm are attached by their acrosome to the surface of the dot. Hyaluronan bound sperms are selected for oocyte injection |
Device: Physiological ICSI
Semen processing is done by double layer density gradient method followed by adding Sperm to the dot of hyaluronan on the PICSI dish, within minutes the bound sperm are attached by their acrosome to the surface of the dot. Hyaluronan bound sperms are selected for oocyte injection
Other Names:
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| Magnetic Activated Cell Sorting (MACS) Semen processing is done by double layer DGC method. The resulted pellet is labeled with Annexin V microbeads followed by separation on MACS column, the eluted fraction contains non apoptotic sperms suitable for oocyte injection. |
Device: Magnetic Activated Cell Sorting
Semen processing is done by double layer density gradient method. The resulted pellet is labeled with Annexin V microbeads followed by separation on MACS column, the eluted fraction contains non apoptotic sperms suitable for oocyte injection.
Other Names:
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Outcome Measures
Primary Outcome Measures
- Gender ratio [36-40 weeks after ICSI]
Neonatal gender ratio
Eligibility Criteria
Criteria
Inclusion Criteria:
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Full Term Delivery
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ICSI cases using DGC for sperm selection
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ICSI cases using MACS for sperm selection
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ICSI cases using PICSI dishes for sperm selection
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Fresh blastocyst stage embryo transfer at
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Fresh ejaculate
Exclusion Criteria:
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Frozen sperm
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Frozen Embryo transfer
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Fresh cleavage stage embryo transfer
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Vanishing Twin Syndrome
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Fetal Reduction
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Preimlantation genetically screened embryo transfer
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Ganin Fertility Center | Cairo | القاهرة | Egypt | 11728 |
Sponsors and Collaborators
- Ganin Fertility Center
- The Cleveland Clinic
- University of the Western Cape
Investigators
- Principal Investigator: khaled Elqusi, BSc, Ganin Fertility Center
- Principal Investigator: Eman Hassanen, Bsc, Ganin Fertility Center
- Principal Investigator: Hanaa Alkhader, ABB( ELD), Ganin Fertility Center
- Principal Investigator: Hosam Zaki, MSc, FRCOG, Ganin Fertility Center
- Study Director: Ralph Henkel, PhD, University of the Western Cape
- Study Chair: Ashok Agarwal, PhD, American Center of Reproductive Medicine, Cleveland Clinic
Study Documents (Full-Text)
None provided.More Information
Publications
- Avendaño C, Franchi A, Taylor S, Morshedi M, Bocca S, Oehninger S. Fragmentation of DNA in morphologically normal human spermatozoa. Fertil Steril. 2009 Apr;91(4):1077-84. doi: 10.1016/j.fertnstert.2008.01.015. Epub 2008 Apr 28.
- Celik-Ozenci C, Jakab A, Kovacs T, Catalanotti J, Demir R, Bray-Ward P, Ward D, Huszar G. Sperm selection for ICSI: shape properties do not predict the absence or presence of numerical chromosomal aberrations. Hum Reprod. 2004 Sep;19(9):2052-9. Epub 2004 Jun 17.
- Komori S, Kato H, Kobayashi S, Koyama K, Isojima S. Transmission of Y chromosomal microdeletions from father to son through intracytoplasmic sperm injection. J Hum Genet. 2002;47(9):465-8.
- McElreavey K, Krausz C. Sex Chromosome Genetics '99. Male infertility and the Y chromosome. Am J Hum Genet. 1999 Apr;64(4):928-33. Review.
- Niederberger C. Absence of de novo Y-chromosome microdeletions in male children conceived through intracytoplasmic sperm injection. J Urol. 2005 Sep;174(3):1046.
- Said TM, Land JA. Effects of advanced selection methods on sperm quality and ART outcome: a systematic review. Hum Reprod Update. 2011 Nov-Dec;17(6):719-33. doi: 10.1093/humupd/dmr032. Epub 2011 Aug 25. Review.
- GFC - 003