IDperform: Predicting the Efficacy of Resistance Exercise Training for Improving Endurance Performance
Study Details
Study Description
Brief Summary
The overall objective of the study is to identify molecular determinants of the efficacy of resistance training for improving endruance performance in elite cyclists
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
For elite endurance athletes, the addition of resistance training to habitual endurance training routines generally leads to improved endurance performance. However, this is not always the case, with several individuals failing to respond in the expected manner. This inter-individual variation is likely associated with inherent cellular and molecular charateristics of skeletal muscle. Indeed, responses to resistance training seem to be associated with muscle biological characteristics such as muscle fiber composition and ribosomal content (during the early phase of resistance training).
The study will investigate the association between muscle fibre composition (type I vs type II muscle fiber proportions) / ribosomal content in m. vastus lateralis and the effects of 10 weeks of resistance training on endurance performance in 52 elite cyclists. Half the participants will perform resistance training in addition to their habitual endurance training routines, the other half will perform endurance training-only. Muscle biopsies will be sampled from m. vastus lateralis before and after the first two weeks of the intervention. For muscle fiber composition, the hypothesis is that larger proportions of type II fibers will be associated with more pronounced benefits of resistance training. For ribosomal content, the hypothesis is that higher rRNA content at baseline and larger increases in rRNA content during the initial part of the intervention will be associated with more pronounced benefits of resistance training. No changes are expected in the non-resistance training control group.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: Resistance training added to endurance training Participants (elite cyclists) will conduct heavy-load resistance training twice a week in addition to their habitual endurance training for ten weeks |
Behavioral: Resistance training
Resistance training sessions twice a week for ten weeks
Other Names:
Behavioral: Endurance training (habitual)
Endurance training for ten weeks (habitual routines)
|
| Other: Endurance training Participants (elite cyclists) will conduct habitual endurance training-only for ten weeks |
Behavioral: Endurance training (habitual)
Endurance training for ten weeks (habitual routines)
|
Outcome Measures
Primary Outcome Measures
- Performance during a 40-minute all-out cycling test [Changes from before the intervention to immediately after the intervention]
Mean power output measured during a 40-minute all-out cycling test
- Performance during a 15-minute all-out cycling test [Changes from before the intervention to immediately after the intervention]
Mean power output measured during a 15-minute all-out cycling test
Secondary Outcome Measures
- Total RNA in muscle tissue (before intervention) [Before the intervention]
Total RNA content in m. vastus lateralis (ug per mg tissue)
- Total RNA in muscle tissue (changes after two weeks) [Changes from before the intervention to immediately after the initial two weeks of resistance training]
Total RNA content in m. vastus lateralis (ug per mg tissue)
- Muscle fiber proportions (before intervention) [Before the intervention]
Muscle fiber proportions in m. vastus lateralis measured using immunohistochemistry
- Muscle fiber proportions (changes after two weeks) [Changes from before the intervention to immediately after the initial two weeks of resistance training]
Muscle fiber proportions in m. vastus lateralis measured using immunohistochemistry
- Muscle fiber cross-sectional area (before intervention) [Before the intervention]
Cross-sectional area of muscle fibres in m. vastus lateralis measured using immunohistochemistry
- Muscle fiber cross-sectional area (changes after two weeks) [Changes from before the intervention to immediately after the initial two weeks of resistance training]
Cross-sectional area of muscle fibres in m. vastus lateralis measured using immunohistochemistry
- Myonuclear number (before intervention) [Before the intervention]
Myonuclei per muscle fiber in m. vastus lateralis measured using immunohistochemistry
- Myonuclear number (changes after two weeks) [Changes from before the intervention to immediately after the initial two weeks of resistance training]
Myonuclei per muscle fiber in m. vastus lateralis measured using immunohistochemistry
- rRNA expression (before intervention) [Before the intervention]
rRNA abundances in m. vastus lateralis measured using qPCR
- rRNA expression (changes after two weeks) [Changes from before the intervention to immediately after the initial two weeks of resistance training]
rRNA abundances in m. vastus lateralis measured using qPCR
- mRNA expression (before intervention) [Before the intervention]
mRNA abundances in m. vastus lateralis measured using qPCR
- mRNA expression (changes after two weeks) [Changes from before the intervention to immediately after the initial two weeks of resistance training]
mRNA abundances in m. vastus lateralis measured using qPCR
- Maximal concentric force production [Changes from before the intervention to immediately after the intervention]
Maximal concentric force production measured using a seated leg press test
- Sprint performance [Changes from before the intervention to immediately after the intervention]
Mean power output measured during a 10-second all-out cycling sprint
- Maximal oxygen consumption [Changes from before the intervention to immediately after the intervention]
Maximal oxygen consumption measured during an incremental cycling exercise test to exhaustion
- Maximal aerobic power output [Changes from before the intervention to immediately after the intervention]
Maximal aerobic power output measured as mean power output during the last minute of an incremental cycling exercise test to exhaustion
- Power output at lactate threshold [Changes from before the intervention to immediately after the intervention]
Power output at 4 mmol blood lactate concentration measured during an incremental cycling exercise test (with 5 minute steps)
- Gross efficiency [Changes from before the intervention to immediately after the intervention]
Contribution of total energy turnover to power output in the fresh and fatigued state
- Fractional utilization of VO2max (incremental test) [Changes from before the intervention to immediately after the intervention]
Fractional utilization of VO2max measured at 4 mmol blood lactate concentrations measured during an incremental cycling exercise test (with 5 minute steps)
- Fractional utilization of VO2max (15-minute performance test) [Changes from before the intervention to immediately after the intervention]
Fractional utilization of VO2max measured during a 15-minute performance test
- Hemoglobin mass [Changes from before the intervention to immediately after the intervention]
Hemoglobin mass measured using CO rebreathing (g)
- Blood volume [Changes from before the intervention to immediately after the intervention]
Blood volume measured using CO rebreathing
- Plasma volume [Changes from before the intervention to immediately after the intervention]
Plasma volume measured using CO rebreathing
- Red blood cell volume [Changes from before the intervention to immediately after the intervention]
Red blood cell volume measured using CO rebreathing
- Hematocrit [Changes from before the intervention to immediately after the intervention]
Hematocrit measured using centrifugation
Other Outcome Measures
- Endurance training volume [Throughout study completion (daily), an average of ten weeks]
Self-reported endurance training volume measured as time-spent in different exercise intensity zones
- Resistance training volume [Throughout study completion (daily), an average of ten weeks]
Self-reported resistance training volume measured as load x repetitions
Eligibility Criteria
Criteria
Inclusion criteria:
-
VO2max > 65 ml/kg/min (male participants)
-
VO2max > 50 ml/kg/min (female participants)
-
7 hours of endurance training per week for the 6 months leading up to the study
Exclusion criteria:
- having conducted regular resistance training for the last 6 months leading up to the study
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Inland Norway University of Applied Sciences | Lillehammer | Inland Norway | Norway | 2613 |
Sponsors and Collaborators
- Inland Norway University of Applied Sciences
Investigators
- Study Chair: Anne S Lofthus, Research Administrator
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- Trainome 2020#025-2