Evaluation of The Effect of 980nm Diode Laser Intracanal Irradiation on Levels of Interleukin-8 Expression
Study Details
Study Description
Brief Summary
This clinical trial will be conducted to evaluate the amount of Il-8 expression before and after the application of intracanal irradiation with diode laser 980 nm versus placebo in failed endodontic cases with periapical lesions.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
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Thorough medical and dental history for the eligible patients
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Thorough intraoral and extraoral diagnosis
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Local anesthesia
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Isolation and removal of any previous filling or restoration
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Removal of old gutta percha and establish patency to the full working length.
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1st sample: will be collected using 3 sterile paper points
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Chemo-mechanical preparation.
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2nd sample collection
• Laser group: The canals will be irradiated with a high-power diode laser, with wavelength of 980 nm, maximum output of 3 watts and coupled with a fiber optic tip of 200 µm diameter.
• Mock laser application (Placebo group): The fiberoptic tip will be inserted inside the root canal, mimicking the laser irradiation, but not activated.
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3rd sample collection
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A piece of dry cotton will be placed in the access cavity, and then will be sealed with glass ionomer filling material
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2nd visit: 1 week later, 4th sample collection then Obturation
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Number of visits & follow up period: Two visits
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Placebo Comparator: Placebo group • Mock laser application (Placebo group): The fiber optic tip will be inserted inside the root canal, mimicking the laser irradiation group, but not activated |
Other: Placebo
After endodontic retreatment, mock laser application (Placebo group) will be applied:
The fiberoptic tip will be inserted inside the root canal, mimicking the laser irradiation group, but not activated
|
| Other: Diode Laser group Intracanal Diode laser application |
Device: Intracanal Diode laser irradiation
After endodontic retreatment, root canals will be irradiated with a 980 nm diode laser coupled with optical fibre 200 μm (Litemedics, Italy) with setting 1.2-watt power, in pulsed mode. The irradiation protocol will be of 5-sec irradiation followed by a 10-sec pause, which constituted one lasing cycle. The lasing cycle will be performed four times for each tooth. The tip will be positioned 1 mm short of the apex. Followed by activation during which it will be slowly dragged at a speed of approximately 2 mm/ sec in a way that the root canals will be irradiated from the apical to the coronal portion, in a helicoidal movement touching the canal walls.
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Outcome Measures
Primary Outcome Measures
- quantification of IL-8 [one week]
- After collection of all the samples, quantification of IL-8 will be determined using ELISA kit before (S-1), after chemo-mechanical preparation (S-2), after intracanal irradiation or placebo effect (S-3) and last after one week of the treatment (S-4). The kit is used according to the manufacturer's recommendations.
Eligibility Criteria
Criteria
Inclusion Criteria:
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failed previously treated endodontic single-rooted teeth with chronic periapical lesions; with radiographic evidence of periapical radiolucency of not less than 1 cm diameter will be selected.
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Teeth with straight root canals.
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No contributory medical history.
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No previous administration of analgesics and/or antibiotics within the previous 2 weeks.
Exclusion Criteria:
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Retreatment cases with complications such as separated instruments, perforations, ledges, resorption, transportation, calcifications, immature apices and over fillings.
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Teeth with curved roots.
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Swelling or sinus tract.
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Mutilated teeth that interfere with proper isolation and seal between visits.
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Subjects with generalized periodontitis, or if the tooth has a probing depth of more than 3 mm.
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Subjects with uncontrolled diabetes or debilitating diseases.
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Pregnant or nursing females.
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Systemic disorders that necessitate antibiotic prophylaxis or prevent the use of Ibuprofen.
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Faculty of Dentistry | Cairo | Egypt | 11553 |
Sponsors and Collaborators
- Cairo University
Investigators
- Principal Investigator: Dina A Morsy, lecturer, Cairo University
- Principal Investigator: Sherif A Elkhodary, assoc. Prof, Cairo University
Study Documents (Full-Text)
None provided.More Information
Publications
- DAMorsy