Organic Pollutants in Pelvic Endometriosis
Study Details
Study Description
Brief Summary
The health toxicity of polycyclic aromatic hydrocarbons (PAHs), present in air and food, generated during energy production and waste incineration, is well known. PAHs can activate the aryl hydrocarbon receptor, which may interact with classic estrogen receptors and modify estrogen-dependent inflammation in endometriosis. There is no data on the hypothetical role of PAHs in the etiopathogenesis of endometriosis. The aim was to compare PAHs concentrations in visceral fat in women with endometriosis and idiopathic infertility.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
A prospective cohort tertiary-center study includes women undergoing laparoscopy due to pelvic endometriosis (arm 1) or/and idiopathic infertility (arm 2). A sample of 1 ml of the greater omentum is collected intraoperatively for detection of 16 PAHs, i.e. acenaphthene, acenaphthylene, anthracene, fluoranthene, fluorene, naphthalene, phenanthrene, pyrene, benz[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[ghi]perylene, benzo[a]pyrene, chrysene, dibenz[a,h]anthracene, and indeno[1,2,3-cd]pyrene, by gas chromatography-isotope dilution mass spectrometry method. The concentration of selected PAHs in visceral fat in both populations will be compared. The correlation of concentrations of selected PAHs with the occurrence of infertility, pelvic pain syndrome, intensity of pain, peritoneal adhesions and the degree of endometriosis will be checked.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: endometriosis Women subjected to elective laparoscopy for pelvic endometriosis |
Diagnostic Test: Detection of PAHs in visceral fat with gas chromatography-isotope dilution mass spectrometry
1 ml sample of visceral fat collected during elective laparoscopy for detection of PAHS by gas chromatography-isotope dilution mass spectrometry
|
| Active Comparator: idiopathic infertility Women subjected to elective laparoscopy for idiopathic infertility |
Diagnostic Test: Detection of PAHs in visceral fat with gas chromatography-isotope dilution mass spectrometry
1 ml sample of visceral fat collected during elective laparoscopy for detection of PAHS by gas chromatography-isotope dilution mass spectrometry
|
Outcome Measures
Primary Outcome Measures
- Accumulation and detection of PAHs in women's visceral fat. [up to 6 months]
Detection and confirmation of the accumulation of PAHs (acenaphthene, acenaphthylene, anthracene, fluoranthene, fluorene, naphthalene, phenanthrene, pyrene, benz[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[ghi]perylene, benzo[a]pyrene, chrysene, dibenz[a,h]anthracene, and indeno[1,2,3-cd]pyrene) in human adipose tissue by gas chromatography-isotope dilution mass spectrometry method.
- Comparison of concentrations of 16 reference PAHs in women in both study arms. [up to 6 months]
Comparison of concentrations of 16 PAHs (acenaphthene, acenaphthylene, anthracene, fluoranthene, fluorene, naphthalene, phenanthrene, pyrene, benz[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[ghi]perylene, benzo[a]pyrene, chrysene, dibenz[a,h]anthracene, and indeno[1,2,3-cd]pyrene) in both study arms using standard statistical methods.
Secondary Outcome Measures
- Evaluation of PAHs concentrations correlation with endometriosis degree [up to 6 months]
Assessment of 16 PAHs concentrations depending on the degree of endometriosis severity using standard statistical methods.
- Evaluation of PAHs concentrations correlation with the intensity of pelvic pain [up to 6 months]
Assessment of 16 PAHs concentrations depending the intensity of pelvic pain in Numeric Rating Scale using standard statistical methods.
- Evaluation of PAHs concentrations correlation with the presence and severity of peritoneal adhesions [up to 6 months]
Assessment of 16 PAHs concentrations depending on the presence and severity of peritoneal adhesions using standard statistical methods.
Eligibility Criteria
Criteria
Inclusion Criteria:
- age 18-45, ii) indications for surgical treatment of endometriosis, iii) indications for invasive diagnostics and surgical treatment of idiopathic infertility.
Exclusion Criteria:
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Jagiellonian University Medical College, Department of Gynecology and Obstetrics | Krakow | Poland | 31-501 |
Sponsors and Collaborators
- Jagiellonian University
Investigators
- Study Chair: Robert Jach, Prof., Jagiellonian University
- Principal Investigator: Iwona Gawron, Ph.D., Jagiellonian University
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 1072.6120.333.2021